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6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*

Chemical structure for 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*
Chemical structure for 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*
Ordering information
Price ()
Catalog Number392
Unit Size
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Additional ordering information
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Fax1-408-733-1304
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight631.67
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Quantum yield0.941
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (53)

OverviewpdfSDSpdfProtocol


CAS
216699-36-4
Molecular weight
631.67
Correction Factor (280 nm)
0.168
Extinction coefficient (cm -1 M -1)
82000
Excitation (nm)
578
Emission (nm)
604
Quantum yield
0.941
6-ROX, SE is the other purified single isomer of 5(6)-ROX, SE. 6-ROX is predominately used for labeling nucleotides and sequencing nucleic acids. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM158.311 µL791.553 µL1.583 mL7.916 mL15.831 mL
5 mM31.662 µL158.311 µL316.621 µL1.583 mL3.166 mL
10 mM15.831 µL79.155 µL158.311 µL791.553 µL1.583 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Quantum yield0.941

References


View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32