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6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*

6-ROX is predominately used as a reference dye for performing PCR detections. However, 6-ROX is very unstable compared to other rhodamine dyes. 6-ROXtra™ has greatly improved stability and water solubility. 6-ROXtra™ has almost identical spectral properties to those of 6-ROX. This 6-ROXtra™ dye is 25 uM solution in 20 mM Tris (pH 8.4), 0.1 mM EDTA and 0.01% Tween® 20. 6-ROXtra™ dye is stable at room temperature for a few weeks, making this fluorescent dye an excellent replacement to the widely used 6-ROX PCR reference dye.

Spectrum

References

View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Page updated on October 14, 2024

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Catalog Number398
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Physical properties

Molecular weight

1034.34

Solvent

DMSO

Spectral properties

Excitation (nm)

578

Emission (nm)

595

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Stability comparison of PCR reference solutions (6-ROX, 6-ROXtra&trade; and 6-ROX analog). Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 &deg;C
Stability comparison of PCR reference solutions (6-ROX, 6-ROXtra&trade; and 6-ROX analog). Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 &deg;C
Stability comparison of PCR reference solutions (6-ROX, 6-ROXtra&trade; and 6-ROX analog). Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 &deg;C
Amplification plot for a dilution series of HeLa cell cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix with Helixyte™ Green and ROX (Blue) or ROXtra™ reference solutions (Orange).