Name: 6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*
Brand: AAT Bioquest
SKU: 398
Price: 348 USD
Availability: InStock

6-ROXtra™ fluorescence reference solution

25 uM for PCR reactions

6-ROX is predominately used as a reference dye for performing PCR detections. However, 6-ROX is very unstable compared to other rhodamine dyes. 6-ROXtra™ has greatly improved stability and water solubility. 6-ROXtra™ has almost identical spectral properties to those of 6-ROX. This 6-ROXtra™ dye is 25 uM solution in 20 mM Tris (pH 8.4), 0.1 mM EDTA and 0.01% Tween® 20. 6-ROXtra™ dye is stable at room temperature for a few weeks, making this fluorescent dye an excellent replacement to the widely used 6-ROX PCR reference dye.

Stability comparison of PCR reference solutions (6-ROX, 6-ROXtra™ and 6-ROX analog). Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 °C

Protocol

SDS

COA

Catalog	Size	Price	Quantity
398	5 ml	Price

References

View all 11 references: Citation Explorer

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides

Authors:

Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2005): 5926

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis

Authors:

Faulds K, Smith WE, Graham D.

Journal:

Anal Chem (2004): 412

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry

Authors:

Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.

Journal:

Proc Natl Acad Sci U S A (2004): 5488

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence

Authors:

Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.

Journal:

Langmuir (2004): 6834

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport

Authors:

Yang JH, Basinger SF, Gross RL, Wu SM.

Journal:

Invest Ophthalmol Vis Sci (2003): 1312

Physical properties

Molecular weight	1034.34
Solvent	DMSO

Spectral properties

Excitation (nm)	578
Emission (nm)	595

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 20, 2025