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6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*

Stability comparison of PCR reference solutions (6-ROX, 6-ROXtra™ and 6-ROX analog). Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 °C
Stability comparison of PCR reference solutions (6-ROX, 6-ROXtra™ and 6-ROX analog). Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 °C
Stability comparison of PCR reference solutions (6-ROX, 6-ROXtra™ and 6-ROX analog). Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 °C
Amplification plot for a dilution series of HeLa cell cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix with Helixyte™ Green and ROX (Blue) or ROXtra™ reference solutions (Orange).
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Physical properties
Molecular weight1034.34
SolventDMSO
Spectral properties
Excitation (nm)578
Emission (nm)595
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
1034.34
Excitation (nm)
578
Emission (nm)
595
6-ROX is predominately used as a reference dye for performing PCR detections. However, 6-ROX is very unstable compared to other rhodamine dyes. 6-ROXtra™ has greatly improved stability and water solubility. 6-ROXtra™ has almost identical spectral properties to those of 6-ROX. This 6-ROXtra™ dye is 25 uM solution in 20 mM Tris (pH 8.4), 0.1 mM EDTA and 0.01% Tween® 20. 6-ROXtra™ dye is stable at room temperature for a few weeks, making this fluorescent dye an excellent replacement to the widely used 6-ROX PCR reference dye.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM96.68 µL483.4 µL966.8 µL4.834 mL9.668 mL
5 mM19.336 µL96.68 µL193.36 µL966.8 µL1.934 mL
10 mM9.668 µL48.34 µL96.68 µL483.4 µL966.8 µL

Molarity calculator

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Spectrum


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Spectral properties

Excitation (nm)578
Emission (nm)595

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References


View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32