6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1034.34 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 578 |
| Emission (nm) | 595 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| 6-ROXtra™ SE *Superior 6-ROX Replacement* |
| 6-ROXtra™ acid |
| Overview |  SDSProtocol |
See also: Cell Structures and Organelles, Nucleus, Chemical Reagents, Classic Dyes, Rhodamines and Rhodamine Derivatives, DNA and RNA Quantitation, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), ROX Reference Dye for Real-Time PCR
Molecular weight 1034.34 | Excitation (nm) 578 | Emission (nm) 595 |
6-ROX is predominately used as a reference dye for performing PCR detections. However, 6-ROX is very unstable compared to other rhodamine dyes. 6-ROXtra™ has greatly improved stability and water solubility. 6-ROXtra™ has almost identical spectral properties to those of 6-ROX. This 6-ROXtra™ dye is 25 uM solution in 20 mM Tris (pH 8.4), 0.1 mM EDTA and 0.01% Tween® 20. 6-ROXtra™ dye is stable at room temperature for a few weeks, making this fluorescent dye an excellent replacement to the widely used 6-ROX PCR reference dye.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 96.68 µL | 483.4 µL | 966.8 µL | 4.834 mL | 9.668 mL |
| 5 mM | 19.336 µL | 96.68 µL | 193.36 µL | 966.8 µL | 1.934 mL |
| 10 mM | 9.668 µL | 48.34 µL | 96.68 µL | 483.4 µL | 966.8 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. Stability comparison of PCR reference solutions (6-ROX, 6-ROXtra™ and 6-ROX analog). Blue bar represents starting fluorescence. Red bar represents remaining fluorescence after 4 weeks at 25 °C
Figure 2. Amplification plot for a dilution series of HeLa cell cDNA amplified in replicate reactions to detect GAPDH using TAQuest™ qPCR Master Mix with Helixyte™ Green and ROX (Blue) or ROXtra™ reference solutions (Orange).
References
View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32