Name: 6-TET BCN
Brand: AAT Bioquest
SKU: 70524
Price: 115 USD
Availability: InStock

6-TET BCN

6-TET BCN is a photostable green fluorescent dye used for site-specific labeling of oligonucleotides and other biomolecules via copper-free click chemistry.

Bioorthogonality: The reaction is selective for azido or tetrazine.
High reaction rate: BCN reacts faster than most other click reactions.
Catalyst-free: The reaction does not require a toxic metal catalyst like copper.
High biocompatibility: The reaction occurs under physiological pH and room temperature.
Versatility: It can be used for site-specific modification of a wide range of macromolecules.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
70524	1 mg	Price

Overview

6-TET BCN is a clickable derivative of 6-Carboxy-2',4,7',7-tetrachlorofluorescein (6-TET), a widely used green fluorescent dye for biomolecule labeling, particularly oligonucleotides. Spectrally, 6-TET is similar to VIC, JOE, and R6G, and is frequently employed in molecular biology and biotechnology for sensitive labeling and detection applications. The incorporation of a PEG spacer in 6-TET BCN helps reduce steric hindrance and minimizes potential interference with target binding sites, thereby enhancing conjugation efficiency and preserving the functionality of the conjugate. The bicyclononyne (BCN) moiety enables strain-promoted azide–alkyne cycloaddition (SPAAC) with azido groups, forming stable triazole linkages under catalyst-free conditions. In addition, unlike dibenzocyclooctyne (DBCO), BCN also reacts efficiently with tetrazines through an inverse electron-demand Diels–Alder (IEDDA) reaction. This reaction is rapid, selective, and bioorthogonal, allowing labeling of biomolecules under physiological conditions without the need for metal catalysts or disruption of native biological processes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET BCN to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	110.059 µL	550.297 µL	1.101 mL	5.503 mL	11.006 mL
5 mM	22.012 µL	110.059 µL	220.119 µL	1.101 mL	2.201 mL
10 mM	11.006 µL	55.03 µL	110.059 µL	550.297 µL	1.101 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	908.6 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Photo-Accelerated Synthesis of Oligo(triazole amide)s.

Authors:

Petropoulos, Alexandros and Charles, Laurence and Becht, Jean-Michel and Schmitt, Michael and Lalevée, Jacques and Lutz, Jean-François

Journal:

Macromolecular rapid communications (2025): e2400759

Modular Synthesis of Dendritic Oligo-Glycerol Cationic Surfactants for Enhanced Antibacterial Efficacy.

Authors:

Hanheiser, Natalie and Jiang, Yuhang and Zoister, Christian and Dimde, Mathias and Achazi, Katharina and Nie, Chuanxiong and Li, Yuanyuan and Haag, Rainer and Singh, Abhishek K

Journal:

Angewandte Chemie (International ed. in English) (2025): e202425069

Synthesis and Reactivity of Oligo(ethylene glycol)-Tethered Morita-Baylis-Hillman Dimers in the Formation of Macrocyclic Structures Showing Remarkable Cytotoxicity.

Authors:

Paolino, Marco and Saletti, Mario and Venditti, Jacopo and Zacchei, Arianna and Donati, Alessandro and Bonechi, Claudia and Giuliani, Germano and Lamponi, Stefania and Cappelli, Andrea

Journal:

Pharmaceuticals (Basel, Switzerland) (2025)

Template-Directed Synthesis and Isolation of Unimolecular Oligo[n]catenanes Using a One-Pot CuAAC "Click" Reaction Approach.

Authors:

Tran, Sheila L and Harlan, Gray H and Puckowitz, David E and Wohlstadter, Nathan E and Zhang, Yipei and Colley, Nathan D and Barnes, Jonathan C

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2025): e202501344

Regulation of Cell Membrane Potential through Supramolecular System for Activating Calcium Ion Channels.

Authors:

Song, Gang and Li, Boying and Yang, Zhiwen and Lin, Hongrui and Cheng, Junjie and Huang, Yiming and Xing, Chengfen and Lv, Fengting and Bai, Haotian and Wang, Shu

Journal:

Journal of the American Chemical Society (2024): 25383-25393

Physical properties

Molecular weight	908.6
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.13
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	521
Emission (nm)	542

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on July 15, 2026