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6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]

6-TET, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. It is also often used for labeling oligos.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM163.621 µL818.103 µL1.636 mL8.181 mL16.362 mL
5 mM32.724 µL163.621 µL327.241 µL1.636 mL3.272 mL
10 mM16.362 µL81.81 µL163.621 µL818.103 µL1.636 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

References

View all 12 references: Citation Explorer
Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098
Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016
Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61
Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91
Page updated on November 3, 2024

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Physical properties

Molecular weight

611.17

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.13

Extinction coefficient (cm -1 M -1)

73000

Excitation (nm)

521

Emission (nm)

542

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

902171-57-7