Rhodamine B CAS 81-88-9

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Physical properties

Molecular weight	479.01
Solvent	DMSO

Spectral properties

Absorbance (nm)	541
Extinction coefficient (cm ^-1 M ^-1)	106000¹
Excitation (nm)	546
Emission (nm)	567
Quantum yield	0.7¹, 0.43²

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

81-88-9

Molecular weight

479.01

Absorbance (nm)

541

Extinction coefficient (cm ^-1 M ^-1)

106000¹

Excitation (nm)

546

Emission (nm)

567

Quantum yield

0.7¹, 0.43²

Rhodamine B (also called Rhodamine 610, Basic Violet 10, or C.I. 45170) is used as a fluorescent staining dye, sometimes in combination with auramine O. The auramine-rhodamine stain is used to demonstrate acid-fast organisms, notably Mycobacterium; Rhodamine B is tunable around 610 nm when used as a laser dye.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B *CAS 81-88-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	208.764 µL	1.044 mL	2.088 mL	10.438 mL	20.876 mL
5 mM	41.753 µL	208.764 µL	417.528 µL	2.088 mL	4.175 mL
10 mM	20.876 µL	104.382 µL	208.764 µL	1.044 mL	2.088 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	541
Extinction coefficient (cm ^-1 M ^-1)	106000¹
Excitation (nm)	546
Emission (nm)	567
Quantum yield	0.7¹, 0.43²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
Rhodamine 800 CAS 137993-41-0	682	704	-
Rhodamine 6G CAS 989-38-8	525	548	116000¹
Sulforhodamine B CAS 3520-42-1	559	577	-
Rhodamine 110 CAS 13558-31-1	500	522	80000
Rhodamine 123 CAS 62669-70-9	508	528	85200
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000

Images

Figure 1. Chemical structure for Rhodamine B *CAS 81-88-9*

Citations

View all 1 citations: Citation Explorer

Optothermal crystallization of hard spheres in an effective bidimensional geometry
Authors: Ruzzi, Vincenzo and Baglioni, Jacopo and Piazza, Roberto
Journal: The Journal of Chemical Physics (2023)

References

View all 39 references: Citation Explorer

Synergetic degradation of rhodamine B at a porous ZnWO4 film electrode by combined electro-oxidation and photocatalysis
Authors: Zhao X, Zhu Y.
Journal: Environ Sci Technol (2006): 3367

Energy Transfer From Chemically Attached Rhodamine 101 to Adsorbed Methylene Blue on Microcrystalline Cellulose Particles
Authors: Rodriguez HB, San Roman E.
Journal: Photochem Photobiol. (2006)

Switching the recognition preference of rhodamine B spirolactam by replacing one atom: design of rhodamine B thiohydrazide for recognition of Hg(II) in aqueous solution
Authors: Zheng H, Qian ZH, Xu L, Yuan FF, Lan LD, Xu JG.
Journal: Org Lett (2006): 859

Inhibition of glycosaminoglycan synthesis using rhodamine B in a mouse model of mucopolysaccharidosis type IIIA
Authors: Roberts AL, Thomas BJ, Wilkinson AS, Fletcher JM, Byers S.
Journal: Pediatr Res (2006): 309

Rhodamine-pink as a genetic marker for yeast populations in wine fermentation
Authors: Ambrona J, Vinagre A, Maqueda M, Alvarez ML, Ramirez M.
Journal: J Agric Food Chem (2006): 2977

New fluorescent rhodamine hydrazone chemosensor for Cu(II) with high selectivity and sensitivity
Authors: Xiang Y, Tong A, Jin P, Ju Y.
Journal: Org Lett (2006): 2863

In vivo monitoring of mercury ions using a rhodamine-based molecular probe
Authors: Ko SK, Yang YK, Tae J, Shin I.
Journal: J Am Chem Soc (2006): 14150

Resonance Raman scattering of rhodamine 6G as calculated using time-dependent density functional theory
Authors: Jensen L, Schatz GC.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5973

pH dependence of the interaction between rhodamine B and the water-soluble poly(sodium 4-styrenesulfonate)
Authors: Moreno-Villoslada I, Jofre M, Mir and a V, Gonzalez R, Sotelo T, Hess S, Rivas BL.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 11809

Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)