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Rhodamine B *CAS 81-88-9*

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Physical properties
Molecular weight479.01
Spectral properties
Absorbance (nm)541
Extinction coefficient (cm -1 M -1)1060001
Excitation (nm)546
Emission (nm)567
Quantum yield0.71, 0.432
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.71, 0.432
Rhodamine B (also called Rhodamine 610, Basic Violet 10, or C.I. 45170) is used as a fluorescent staining dye, sometimes in combination with auramine O. The auramine-rhodamine stain is used to demonstrate acid-fast organisms, notably Mycobacterium; Rhodamine B is tunable around 610 nm when used as a laser dye.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B *CAS 81-88-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM208.764 µL1.044 mL2.088 mL10.438 mL20.876 mL
5 mM41.753 µL208.764 µL417.528 µL2.088 mL4.175 mL
10 mM20.876 µL104.382 µL208.764 µL1.044 mL2.088 mL

Molarity calculator

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Spectral properties

Absorbance (nm)541
Extinction coefficient (cm -1 M -1)1060001
Excitation (nm)546
Emission (nm)567
Quantum yield0.71, 0.432

Product Family

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Rhodamine 800 *CAS 137993-41-0*682704-
Rhodamine 6G *CAS 989-38-8*5255481160001
Sulforhodamine B *CAS 3520-42-1*559577-
Rhodamine 110 *CAS 13558-31-1*50052280000
Rhodamine 123 *CAS 62669-70-9*50852885200
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000



View all 1 citations: Citation Explorer
Optothermal crystallization of hard spheres in an effective bidimensional geometry
Authors: Ruzzi, Vincenzo and Baglioni, Jacopo and Piazza, Roberto
Journal: The Journal of Chemical Physics (2023)


View all 39 references: Citation Explorer
Synergetic degradation of rhodamine B at a porous ZnWO4 film electrode by combined electro-oxidation and photocatalysis
Authors: Zhao X, Zhu Y.
Journal: Environ Sci Technol (2006): 3367
Energy Transfer From Chemically Attached Rhodamine 101 to Adsorbed Methylene Blue on Microcrystalline Cellulose Particles
Authors: Rodriguez HB, San Roman E.
Journal: Photochem Photobiol. (2006)
Switching the recognition preference of rhodamine B spirolactam by replacing one atom: design of rhodamine B thiohydrazide for recognition of Hg(II) in aqueous solution
Authors: Zheng H, Qian ZH, Xu L, Yuan FF, Lan LD, Xu JG.
Journal: Org Lett (2006): 859
Inhibition of glycosaminoglycan synthesis using rhodamine B in a mouse model of mucopolysaccharidosis type IIIA
Authors: Roberts AL, Thomas BJ, Wilkinson AS, Fletcher JM, Byers S.
Journal: Pediatr Res (2006): 309
Rhodamine-pink as a genetic marker for yeast populations in wine fermentation
Authors: Ambrona J, Vinagre A, Maqueda M, Alvarez ML, Ramirez M.
Journal: J Agric Food Chem (2006): 2977
New fluorescent rhodamine hydrazone chemosensor for Cu(II) with high selectivity and sensitivity
Authors: Xiang Y, Tong A, Jin P, Ju Y.
Journal: Org Lett (2006): 2863
In vivo monitoring of mercury ions using a rhodamine-based molecular probe
Authors: Ko SK, Yang YK, Tae J, Shin I.
Journal: J Am Chem Soc (2006): 14150
Resonance Raman scattering of rhodamine 6G as calculated using time-dependent density functional theory
Authors: Jensen L, Schatz GC.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5973
pH dependence of the interaction between rhodamine B and the water-soluble poly(sodium 4-styrenesulfonate)
Authors: Moreno-Villoslada I, Jofre M, Mir and a V, Gonzalez R, Sotelo T, Hess S, Rivas BL.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 11809
Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)