Rhodamine B *CAS 81-88-9*
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Physical properties
Molecular weight | 479.01 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 541 |
Extinction coefficient (cm -1 M -1) | 1060001 |
Excitation (nm) | 546 |
Emission (nm) | 567 |
Quantum yield | 0.71, 0.432 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
CAS 81-88-9 | Molecular weight 479.01 | Absorbance (nm) 541 | Extinction coefficient (cm -1 M -1) 1060001 | Excitation (nm) 546 | Emission (nm) 567 | Quantum yield 0.71, 0.432 |
Rhodamine B (also called Rhodamine 610, Basic Violet 10, or C.I. 45170) is used as a fluorescent staining dye, sometimes in combination with auramine O. The auramine-rhodamine stain is used to demonstrate acid-fast organisms, notably Mycobacterium; Rhodamine B is tunable around 610 nm when used as a laser dye.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B *CAS 81-88-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 208.764 µL | 1.044 mL | 2.088 mL | 10.438 mL | 20.876 mL |
5 mM | 41.753 µL | 208.764 µL | 417.528 µL | 2.088 mL | 4.175 mL |
10 mM | 20.876 µL | 104.382 µL | 208.764 µL | 1.044 mL | 2.088 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 541 |
Extinction coefficient (cm -1 M -1) | 1060001 |
Excitation (nm) | 546 |
Emission (nm) | 567 |
Quantum yield | 0.71, 0.432 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Rhodamine 800 *CAS 137993-41-0* | 682 | 704 | - |
Rhodamine 6G *CAS 989-38-8* | 525 | 548 | 1160001 |
Sulforhodamine B *CAS 3520-42-1* | 559 | 577 | - |
Rhodamine 110 *CAS 13558-31-1* | 500 | 522 | 80000 |
Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 |
Rhodamine aldehyde [5-TAMRA aldehyde] | 552 | 578 | 90000 |
References
View all 39 references: Citation Explorer
Synergetic degradation of rhodamine B at a porous ZnWO4 film electrode by combined electro-oxidation and photocatalysis
Authors: Zhao X, Zhu Y.
Journal: Environ Sci Technol (2006): 3367
Authors: Zhao X, Zhu Y.
Journal: Environ Sci Technol (2006): 3367
Energy Transfer From Chemically Attached Rhodamine 101 to Adsorbed Methylene Blue on Microcrystalline Cellulose Particles
Authors: Rodriguez HB, San Roman E.
Journal: Photochem Photobiol. (2006)
Authors: Rodriguez HB, San Roman E.
Journal: Photochem Photobiol. (2006)
Switching the recognition preference of rhodamine B spirolactam by replacing one atom: design of rhodamine B thiohydrazide for recognition of Hg(II) in aqueous solution
Authors: Zheng H, Qian ZH, Xu L, Yuan FF, Lan LD, Xu JG.
Journal: Org Lett (2006): 859
Authors: Zheng H, Qian ZH, Xu L, Yuan FF, Lan LD, Xu JG.
Journal: Org Lett (2006): 859
Inhibition of glycosaminoglycan synthesis using rhodamine B in a mouse model of mucopolysaccharidosis type IIIA
Authors: Roberts AL, Thomas BJ, Wilkinson AS, Fletcher JM, Byers S.
Journal: Pediatr Res (2006): 309
Authors: Roberts AL, Thomas BJ, Wilkinson AS, Fletcher JM, Byers S.
Journal: Pediatr Res (2006): 309
Rhodamine-pink as a genetic marker for yeast populations in wine fermentation
Authors: Ambrona J, Vinagre A, Maqueda M, Alvarez ML, Ramirez M.
Journal: J Agric Food Chem (2006): 2977
Authors: Ambrona J, Vinagre A, Maqueda M, Alvarez ML, Ramirez M.
Journal: J Agric Food Chem (2006): 2977
New fluorescent rhodamine hydrazone chemosensor for Cu(II) with high selectivity and sensitivity
Authors: Xiang Y, Tong A, Jin P, Ju Y.
Journal: Org Lett (2006): 2863
Authors: Xiang Y, Tong A, Jin P, Ju Y.
Journal: Org Lett (2006): 2863
In vivo monitoring of mercury ions using a rhodamine-based molecular probe
Authors: Ko SK, Yang YK, Tae J, Shin I.
Journal: J Am Chem Soc (2006): 14150
Authors: Ko SK, Yang YK, Tae J, Shin I.
Journal: J Am Chem Soc (2006): 14150
Resonance Raman scattering of rhodamine 6G as calculated using time-dependent density functional theory
Authors: Jensen L, Schatz GC.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5973
Authors: Jensen L, Schatz GC.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5973
pH dependence of the interaction between rhodamine B and the water-soluble poly(sodium 4-styrenesulfonate)
Authors: Moreno-Villoslada I, Jofre M, Mir and a V, Gonzalez R, Sotelo T, Hess S, Rivas BL.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 11809
Authors: Moreno-Villoslada I, Jofre M, Mir and a V, Gonzalez R, Sotelo T, Hess S, Rivas BL.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 11809
Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
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A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets