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Sulforhodamine B *CAS 3520-42-1*

Chemical structure for Sulforhodamine B *CAS 3520-42-1*
Chemical structure for Sulforhodamine B *CAS 3520-42-1*
Ordering information
Price ()
Catalog Number72
Unit Size
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Additional ordering information
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Fax1-408-733-1304
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight580.65
SolventWater
Spectral properties
Absorbance (nm)220
Excitation (nm)559
Emission (nm)577
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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6-dR110 [6-Carboxy-4,7-dichlororhodamine 110]
Bis-L-Aspartic acid Beta-rhodamine 110
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Show More (54)

OverviewpdfSDSpdfProtocol


CAS
3520-42-1
Molecular weight
580.65
Absorbance (nm)
220
Excitation (nm)
559
Emission (nm)
577
Sulforhodamine B, also known as Kiton Red, is primarily used as a polar tracer. It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Sulforhodamine B *CAS 3520-42-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM172.221 µL861.104 µL1.722 mL8.611 mL17.222 mL
5 mM34.444 µL172.221 µL344.442 µL1.722 mL3.444 mL
10 mM17.222 µL86.11 µL172.221 µL861.104 µL1.722 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)220
Excitation (nm)559
Emission (nm)577

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Rhodamine B *CAS 81-88-9*5465681060001
Sulforhodamine G *CAS 5873-16-5*531552-
Sulforhodamine 101 *CAS 60311-02-6*578593139000

Citations


View all 1 citations: Citation Explorer
Flow characterization and mixing performance of weakly-shear-thinning fluid flows in a microfluidic oscillator
Authors: Sun, Chen-li and Lin, Yu Jen and Rau, Chia-I and Chiu, Shao-Yu
Journal: Journal of Non-Newtonian Fluid Mechanics (2016)

References


View all 26 references: Citation Explorer
Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633
Sulforhodamine B assay and chemosensitivity
Authors: Voigt W., undefined
Journal: Methods Mol Med (2005): 39
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Authors: Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F.
Journal: Nat Methods (2004): 31
Comparison of the sulforhodamine B assay and the clonogenic assay for in vitro chemoradiation studies
Authors: Pauwels B, Korst AE, de Pooter CM, Pattyn GG, Lambrechts HA, Baay MF, Lardon F, Vermorken JB.
Journal: Cancer Chemother Pharmacol (2003): 221
Formation and identification of intermediates in the visible-light-assisted photodegradation of sulforhodamine-B dye in aqueous TiO2 dispersion
Authors: Chen C, Zhao W, Li J, Zhao J, Hidaka H, Serpone N.
Journal: Environ Sci Technol (2002): 3604
Carbachol-induced fluid movement through methazolamide-sensitive bicarbonate production in rat parotid intralobular ducts: quantitative analysis of fluorescence images using fluorescent dye sulforhodamine under a confocal laser scanning microscope
Authors: Nakamoto T, Shiba Y, Hirono C, Sugita M, Takemoto K, Iwasa Y, Akagawa Y.
Journal: Eur J Cell Biol (2002): 497
Forskolin-induced clearance of the fluorescent dye sulforhodamine from rat parotid intralobular duct lumen: visualization of the secretory function under a confocal laser scanning microscope
Authors: Nakamoto T, Hirono C, Sugita M, Takemoto K, Iwasa Y, Akagawa Y, Shiba Y.
Journal: J Membr Biol (2002): 189
Chemistry of sulforhodamine--amine conjugates
Authors: Corrie JE, Davis CT, Eccleston JF.
Journal: Bioconjug Chem (2001): 186
Diffuse distribution of sulforhodamine-labeled neurons during serotonin-evoked locomotion in the neonatal rat thoracolumbar spinal cord
Authors: Cina C, Hochman S.
Journal: J Comp Neurol (2000): 590
Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay
Authors: Wittig A, Sauerwein W, Poller F, Fuhrmann C, Hideghety K, Streffer C.
Journal: Int J Radiat Biol (1998): 679