Sulforhodamine B CAS 3520-42-1

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Physical properties

Molecular weight	580.65
Solvent	Water

Spectral properties

Absorbance (nm)	220
Excitation (nm)	559
Emission (nm)	577

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

3520-42-1

Molecular weight

580.65

Absorbance (nm)

220

Excitation (nm)

559

Emission (nm)

577

Sulforhodamine B, also known as Kiton Red, is primarily used as a polar tracer. It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Sulforhodamine B *CAS 3520-42-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	172.221 µL	861.104 µL	1.722 mL	8.611 mL	17.222 mL
5 mM	34.444 µL	172.221 µL	344.442 µL	1.722 mL	3.444 mL
10 mM	17.222 µL	86.11 µL	172.221 µL	861.104 µL	1.722 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Absorbance (nm)	220
Excitation (nm)	559
Emission (nm)	577

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
Rhodamine B CAS 81-88-9	546	567	106000¹
Sulforhodamine G CAS 5873-16-5	531	552	-
Sulforhodamine 101 CAS 60311-02-6	578	593	139000

Images

Figure 1. Chemical structure for Sulforhodamine B *CAS 3520-42-1*

Citations

View all 1 citations: Citation Explorer

Flow characterization and mixing performance of weakly-shear-thinning fluid flows in a microfluidic oscillator
Authors: Sun, Chen-li and Lin, Yu Jen and Rau, Chia-I and Chiu, Shao-Yu
Journal: Journal of Non-Newtonian Fluid Mechanics (2016)

References

View all 26 references: Citation Explorer

Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques
Authors: Nagatani H, Suzuki S, Fermin DJ, Girault HH, Nakatani K.
Journal: Anal Bioanal Chem (2006): 633

Sulforhodamine B assay and chemosensitivity
Authors: Voigt W., undefined
Journal: Methods Mol Med (2005): 39

Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Authors: Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F.
Journal: Nat Methods (2004): 31

Comparison of the sulforhodamine B assay and the clonogenic assay for in vitro chemoradiation studies
Authors: Pauwels B, Korst AE, de Pooter CM, Pattyn GG, Lambrechts HA, Baay MF, Lardon F, Vermorken JB.
Journal: Cancer Chemother Pharmacol (2003): 221

Formation and identification of intermediates in the visible-light-assisted photodegradation of sulforhodamine-B dye in aqueous TiO2 dispersion
Authors: Chen C, Zhao W, Li J, Zhao J, Hidaka H, Serpone N.
Journal: Environ Sci Technol (2002): 3604

Carbachol-induced fluid movement through methazolamide-sensitive bicarbonate production in rat parotid intralobular ducts: quantitative analysis of fluorescence images using fluorescent dye sulforhodamine under a confocal laser scanning microscope
Authors: Nakamoto T, Shiba Y, Hirono C, Sugita M, Takemoto K, Iwasa Y, Akagawa Y.
Journal: Eur J Cell Biol (2002): 497

Forskolin-induced clearance of the fluorescent dye sulforhodamine from rat parotid intralobular duct lumen: visualization of the secretory function under a confocal laser scanning microscope
Authors: Nakamoto T, Hirono C, Sugita M, Takemoto K, Iwasa Y, Akagawa Y, Shiba Y.
Journal: J Membr Biol (2002): 189

Chemistry of sulforhodamine--amine conjugates
Authors: Corrie JE, Davis CT, Eccleston JF.
Journal: Bioconjug Chem (2001): 186

Diffuse distribution of sulforhodamine-labeled neurons during serotonin-evoked locomotion in the neonatal rat thoracolumbar spinal cord
Authors: Cina C, Hochman S.
Journal: J Comp Neurol (2000): 590

Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay
Authors: Wittig A, Sauerwein W, Poller F, Fuhrmann C, Hideghety K, Streffer C.
Journal: Int J Radiat Biol (1998): 679