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Rhodamine 123 *CAS 62669-70-9*

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Physical properties
Molecular weight380.82
Spectral properties
Absorbance (nm)497
Extinction coefficient (cm -1 M -1)85200
Excitation (nm)508
Emission (nm)528
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Among them rhodamine 123 is the most frequently used mitochondrial stain. It is utilized for staining a wide variety of cells, including plant cells and bacteria. Like JC-1, rhodamine 123 is also used for measuring mitochondrial membrane potential besides its selective mitochondrial staining. It is possible to use rhodamine 123 for detecting cancer cells since it is selectively localized in cancer cells, and its fluorescence is closely related to the alterations in mitochondrial distribution. Rhodamine 123 is commonly used in flow cytometry as functional reporter for Pgp. Recent reports indicate rhodamine 123 may also be a substrate of MRP1. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. The spectral properties of rhodamine 123 similar to those of FITC make the use of rhodamine 123 quite convenient as almost all fluorescence instruments are equipped with excitation source and filter set of FITC. Rhodamine 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 123 *CAS 62669-70-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM262.591 µL1.313 mL2.626 mL13.13 mL26.259 mL
5 mM52.518 µL262.591 µL525.183 µL2.626 mL5.252 mL
10 mM26.259 µL131.296 µL262.591 µL1.313 mL2.626 mL

Molarity calculator

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Spectral properties

Absorbance (nm)497
Extinction coefficient (cm -1 M -1)85200
Excitation (nm)508
Emission (nm)528

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Rhodamine 800 *CAS 137993-41-0*682704-
Rhodamine B *CAS 81-88-9*5465671060001
Rhodamine 6G *CAS 989-38-8*5255481160001
Rhodamine 110 *CAS 13558-31-1*50052280000
Dihydrorhodamine 123 *CAS 109244-58-8*50852885200
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000



View all 98 references: Citation Explorer
Relationship between excretion clearance of rhodamine 123 and P-glycoprotein (Pgp) expression induced by representative Pgp inducers
Authors: Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.
Journal: Biol Pharm Bull (2006): 779
Determination of rhodamine 123 in cell lysate by high-performance liquid chromatography with visible wavelength detection
Authors: Song JP., undefined
Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban (2006): 610
Rhodamine 123 permeability through the catfish intestinal wall: Relationship to thermal acclimation and acute temperature change
Authors: Kleinow KM, Johnston BD, Holmes EP, McCarrol ME.
Journal: Comp Biochem Physiol C Toxicol Pharmacol (2006): 205
The effect of Mdr1 induction on the pharmacokinetics of rhodamine 123 in rats
Authors: Micuda S, Mundlova L, Mokry J, Osterreicher J, Cermanova J, Cizkova D, Martinkova J.
Journal: Basic Clin Pharmacol Toxicol (2005): 257
Rhodamine-123: therapy for hormone refractory prostate cancer, a phase I clinical trial
Authors: Jones LW, Narayan KS, Shapiro CE, Sweatman TW.
Journal: J Chemother (2005): 435
Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767
Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430
Assessment of drug interactions in hepatobiliary transport using rhodamine 123 in sandwich-cultured rat hepatocytes
Authors: Annaert PP, Brouwer KL.
Journal: Drug Metab Dispos (2005): 388
Determination of Rhodamine 123 in cell lysate by HPLC with visible wavelength detection
Authors: Iqbal T, Kinjo M, Dowling TC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 259
Alterations in respiration rate of isolated rainbow trout hepatocytes exposed to the P-glycoprotein substrate rhodamine 123
Authors: Bains OS, Kennedy CJ.
Journal: Toxicology (2005): 87