Rhodamine 123 *CAS 62669-70-9*
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Physical properties
Molecular weight | 380.82 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 497 |
Extinction coefficient (cm -1 M -1) | 85200 |
Excitation (nm) | 508 |
Emission (nm) | 528 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
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See also: Mitochondria, Rhodamines and Rhodamine Derivatives
CAS 62669-70-9 | Molecular weight 380.82 | Absorbance (nm) 497 | Extinction coefficient (cm -1 M -1) 85200 | Excitation (nm) 508 | Emission (nm) 528 |
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Among them rhodamine 123 is the most frequently used mitochondrial stain. It is utilized for staining a wide variety of cells, including plant cells and bacteria. Like JC-1, rhodamine 123 is also used for measuring mitochondrial membrane potential besides its selective mitochondrial staining. It is possible to use rhodamine 123 for detecting cancer cells since it is selectively localized in cancer cells, and its fluorescence is closely related to the alterations in mitochondrial distribution. Rhodamine 123 is commonly used in flow cytometry as functional reporter for Pgp. Recent reports indicate rhodamine 123 may also be a substrate of MRP1. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. The spectral properties of rhodamine 123 similar to those of FITC make the use of rhodamine 123 quite convenient as almost all fluorescence instruments are equipped with excitation source and filter set of FITC. Rhodamine 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 123 *CAS 62669-70-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 262.591 µL | 1.313 mL | 2.626 mL | 13.13 mL | 26.259 mL |
5 mM | 52.518 µL | 262.591 µL | 525.183 µL | 2.626 mL | 5.252 mL |
10 mM | 26.259 µL | 131.296 µL | 262.591 µL | 1.313 mL | 2.626 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 497 |
Extinction coefficient (cm -1 M -1) | 85200 |
Excitation (nm) | 508 |
Emission (nm) | 528 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Rhodamine 800 *CAS 137993-41-0* | 682 | 704 | - |
Rhodamine B *CAS 81-88-9* | 546 | 567 | 1060001 |
Rhodamine 6G *CAS 989-38-8* | 525 | 548 | 1160001 |
Rhodamine 110 *CAS 13558-31-1* | 500 | 522 | 80000 |
Dihydrorhodamine 123 *CAS 109244-58-8* | 508 | 528 | 85200 |
Rhodamine aldehyde [5-TAMRA aldehyde] | 552 | 578 | 90000 |
References
View all 98 references: Citation Explorer
Relationship between excretion clearance of rhodamine 123 and P-glycoprotein (Pgp) expression induced by representative Pgp inducers
Authors: Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.
Journal: Biol Pharm Bull (2006): 779
Authors: Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.
Journal: Biol Pharm Bull (2006): 779
Determination of rhodamine 123 in cell lysate by high-performance liquid chromatography with visible wavelength detection
Authors: Song JP., undefined
Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban (2006): 610
Authors: Song JP., undefined
Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban (2006): 610
Rhodamine 123 permeability through the catfish intestinal wall: Relationship to thermal acclimation and acute temperature change
Authors: Kleinow KM, Johnston BD, Holmes EP, McCarrol ME.
Journal: Comp Biochem Physiol C Toxicol Pharmacol (2006): 205
Authors: Kleinow KM, Johnston BD, Holmes EP, McCarrol ME.
Journal: Comp Biochem Physiol C Toxicol Pharmacol (2006): 205
Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020
Alterations in respiration rate of isolated rainbow trout hepatocytes exposed to the P-glycoprotein substrate rhodamine 123
Authors: Bains OS, Kennedy CJ.
Journal: Toxicology (2005): 87
Authors: Bains OS, Kennedy CJ.
Journal: Toxicology (2005): 87
Determination of Rhodamine 123 in cell lysate by HPLC with visible wavelength detection
Authors: Iqbal T, Kinjo M, Dowling TC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 259
Authors: Iqbal T, Kinjo M, Dowling TC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 259
Assessment of drug interactions in hepatobiliary transport using rhodamine 123 in sandwich-cultured rat hepatocytes
Authors: Annaert PP, Brouwer KL.
Journal: Drug Metab Dispos (2005): 388
Authors: Annaert PP, Brouwer KL.
Journal: Drug Metab Dispos (2005): 388
Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430
Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767
The effect of Mdr1 induction on the pharmacokinetics of rhodamine 123 in rats
Authors: Micuda S, Mundlova L, Mokry J, Osterreicher J, Cermanova J, Cizkova D, Martinkova J.
Journal: Basic Clin Pharmacol Toxicol (2005): 257
Authors: Micuda S, Mundlova L, Mokry J, Osterreicher J, Cermanova J, Cizkova D, Martinkova J.
Journal: Basic Clin Pharmacol Toxicol (2005): 257