Name: Rhodamine 123 *CAS 62669-70-9*
Brand: AAT Bioquest
SKU: 22210
Price: 90 USD
Availability: InStock

Rhodamine 123

CAS 62669-70-9

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Among them rhodamine 123 is the most frequently used mitochondrial stain. It is utilized for staining a wide variety of cells, including plant cells and bacteria. Like JC-1, rhodamine 123 is also used for measuring mitochondrial membrane potential besides its selective mitochondrial staining. It is possible to use rhodamine 123 for detecting cancer cells since it is selectively localized in cancer cells, and its fluorescence is closely related to the alterations in mitochondrial distribution. Rhodamine 123 is commonly used in flow cytometry as functional reporter for Pgp. Recent reports indicate rhodamine 123 may also be a substrate of MRP1. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. The spectral properties of rhodamine 123 similar to those of FITC make the use of rhodamine 123 quite convenient as almost all fluorescence instruments are equipped with excitation source and filter set of FITC. Rhodamine 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA.

Catalog	Size	Price	Quantity
22210	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 123 *CAS 62669-70-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	262.591 µL	1.313 mL	2.626 mL	13.13 mL	26.259 mL
5 mM	52.518 µL	262.591 µL	525.183 µL	2.626 mL	5.252 mL
10 mM	26.259 µL	131.296 µL	262.591 µL	1.313 mL	2.626 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	380.82 g/mol	=	mL	x	mM	=	mmol

References

View all 98 references: Citation Explorer

Relationship between excretion clearance of rhodamine 123 and P-glycoprotein (Pgp) expression induced by representative Pgp inducers

Authors:

Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.

Journal:

Biol Pharm Bull (2006): 779

Determination of rhodamine 123 in cell lysate by high-performance liquid chromatography with visible wavelength detection

Authors:

Song JP., undefined

Journal:

Zhong Nan Da Xue Xue Bao Yi Xue Ban (2006): 610

Rhodamine 123 permeability through the catfish intestinal wall: Relationship to thermal acclimation and acute temperature change

Authors:

Kleinow KM, Johnston BD, Holmes EP, McCarrol ME.

Journal:

Comp Biochem Physiol C Toxicol Pharmacol (2006): 205

The effect of Mdr1 induction on the pharmacokinetics of rhodamine 123 in rats

Authors:

Micuda S, Mundlova L, Mokry J, Osterreicher J, Cermanova J, Cizkova D, Martinkova J.

Journal:

Basic Clin Pharmacol Toxicol (2005): 257

Rhodamine-123: therapy for hormone refractory prostate cancer, a phase I clinical trial

Authors:

Jones LW, Narayan KS, Shapiro CE, Sweatman TW.

Journal:

J Chemother (2005): 435

Physical properties

Molecular weight	380.82
Solvent	DMSO

Spectral properties

Absorbance (nm)	497
Extinction coefficient (cm ^-1 M ^-1)	85200
Excitation (nm)	508
Emission (nm)	528

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	62669-70-9

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Page updated on October 13, 2025