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Rhodamine 800 *CAS 137993-41-0*

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Physical properties
Molecular weight495.95
Spectral properties
Excitation (nm)682
Emission (nm)704
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Show More (41)


See also: Mitochondria
Molecular weight
Excitation (nm)
Emission (nm)
Rh 800 was originally developed as a laser dye. It is one of the few rhodamines that have near infrared fluorescence. It is also used for staining mitochondria.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 800 *CAS 137993-41-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM201.633 µL1.008 mL2.016 mL10.082 mL20.163 mL
5 mM40.327 µL201.633 µL403.266 µL2.016 mL4.033 mL
10 mM20.163 µL100.817 µL201.633 µL1.008 mL2.016 mL

Molarity calculator

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Spectral properties

Excitation (nm)682
Emission (nm)704

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Rhodamine B *CAS 81-88-9*5465671060001
Rhodamine 6G *CAS 989-38-8*5255481160001
Rhodamine 110 *CAS 13558-31-1*50052280000
Rhodamine 123 *CAS 62669-70-9*50852885200
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000



View all 3 citations: Citation Explorer
The impact of retinol loading and surface charge on the hepatic delivery of lipid nanoparticles
Authors: Pan, Tai-Long and Wang, Pei-Wen and Hung, Chi-Feng and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Colloids and Surfaces B: Biointerfaces (2016): 584--594
Cationic additives in nanosystems activate cytotoxicity and inflammatory response of human neutrophils: lipid nanoparticles versus polymeric nanoparticles
Authors: Hwang, Tsong-Long and Aljuffali, Ibrahim A and Lin, Chwan-Fwu and Chang, Yuan-Ting and Fang, Jia-You
Journal: International journal of nanomedicine (2015): 371
Combined strategies of apomorphine diester prodrugs and nanostructured lipid carriers for efficient brain targeting
Authors: Liu, Kuo-Sheng and Wen, Chih-Jen and Yen, Tzu-Chen and Sung, KC and Ku, Ming-Chuan and Wang, Jhi-Joung and Fang, Jia-You
Journal: Nanotechnology (2012): 095103


View all 39 references: Citation Explorer
Nonphotochemical hole-burning study of selectively stained normal and cancerous human ovarian tissues
Authors: Matsuzaki S, Hayes JM, Benbrook DM, Jankowiak R.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 16124
Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Formation and dissociation of rhodamine 800 dimers in water: steady-state and ultrafast spectroscopic study
Authors: Sekiguchi K, Yamaguchi S, Tahara T.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 2601
Up-regulation of P-glycoprotein expression by glutathione depletion-induced oxidative stress in rat brain microvessel endothelial cells
Authors: Hong H, Lu Y, Ji ZN, Liu GQ.
Journal: J Neurochem (2006): 1465
Modulation of drug-stimulated ATPase activity of human MDR1/P-glycoprotein by cholesterol
Authors: Kimura Y, Kioka N, Kato H, Matsuo M, Ueda K.
Journal: Biochem J. (2006)
Interface-specific chi(4) coherent Raman spectroscopy in the frequency domain
Authors: Yamaguchi S, Tahara T.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2005): 24211
Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy
Authors: Eggeling C, Volkmer A, Seidel CA.
Journal: Chemphyschem (2005): 791
Elevated oxidative stress and reciprocal reduction of vascular endothelial growth factor levels with severity of COPD
Authors: Kanazawa H, Yoshikawa J.
Journal: Chest (2005): 3191
A Fenton reaction at the endoplasmic reticulum is involved in the redox control of hypoxia-inducible gene expression
Authors: Liu Q, Berchner-Pfannschmidt U, Moller U, Brecht M, Wotzlaw C, Acker H, Jungermann K, Kietzmann T.
Journal: Proc Natl Acad Sci U S A (2004): 4302
Flow injection chemiluminescence determination of dihydralazine sulphate based on permanganate oxidation sensitized by rhodamine B
Authors: Yang XF, Wu DB, Li H.
Journal: Luminescence (2004): 322