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TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*

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Physical properties
Molecular weight500.93
Spectral properties
Excitation (nm)552
Emission (nm)574
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Show More (47)


Molecular weight
Excitation (nm)
Emission (nm)
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM199.629 µL998.143 µL1.996 mL9.981 mL19.963 mL
5 mM39.926 µL199.629 µL399.257 µL1.996 mL3.993 mL
10 mM19.963 µL99.814 µL199.629 µL998.143 µL1.996 mL

Molarity calculator

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Spectral properties

Excitation (nm)552
Emission (nm)574



View all 13 citations: Citation Explorer
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Authors: Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.
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Authors: Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.
Journal: Eur J Pharmacol (2006): 69
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Authors: Forster K, Paul I, Solenkova N, Staudt A, Cohen MV, Downey JM, Felix SB, Krieg T.
Journal: Basic Res Cardiol (2006): 319
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Authors: Criddle DN, Murphy J, Fistetto G, Barrow S, Tepikin AV, Neoptolemos JP, Sutton R, Petersen OH.
Journal: Gastroenterology (2006): 781
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Authors: Greco NJ, Seetharaman S, Kurtz J, Lee WR, Moroff G.
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Journal: Cell Death Differ (2006): 151
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Journal: Cardiovasc Res (2006): 354
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Authors: Kadono T, Zhang XQ, Srinivasan S, Ishida H, Barry WH, Benjamin IJ.
Journal: J Mol Cell Cardiol (2006): 783
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Authors: Ouyang YB, Xu LJ, Sun YJ, Giffard RG.
Journal: Cell Stress Chaperones (2006): 180