Name: TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*
Brand: AAT Bioquest
SKU: 22221
Price: 113 USD
Availability: InStock

TMRM

Tetramethylrhodamine methyl ester; CAS#: 115532-50-8

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22221	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	199.629 µL	998.143 µL	1.996 mL	9.981 mL	19.963 mL
5 mM	39.926 µL	199.629 µL	399.257 µL	1.996 mL	3.993 mL
10 mM	19.963 µL	99.814 µL	199.629 µL	998.143 µL	1.996 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	500.93 g/mol	=	mL	x	mM	=	mmol

Citations

View all 14 citations: Citation Explorer

Effects of Pulsed Radiofrequency Current and Thermal Condition on the Expression of $\beta$-Endorphin in Human Monocytic Cells

Authors:

Nishioka, Akira and Azma, Toshiharu and Mieda, Tsutomu and Mio, Yasushi

Journal:

NeuroSci (2025): 67

Chronic calcium signaling in IgE+ B cells limits plasma cell differentiation and survival

Authors:

Newman, Rebecca and Tolar, Pavel

Journal:

Immunity (2021): 2756--2771

Genome-wide screens identify calcium signaling as a key regulator of IgE+ plasma cell differentiation and survival

Authors:

Newman, Rebecca and Tolar, Pavel

Journal:

Available at SSRN 3800380 (2021)

Low-dose sorafenib acts as a mitochondrial uncoupler and ameliorates nonalcoholic steatohepatitis

Authors:

Jian, Chongshu and Fu, Jiajun and Cheng, Xu and Shen, Li-Jun and Ji, Yan-Xiao and Wang, Xiaoming and Pan, Shan and Tian, Han and Tian, Song and Liao, Rufang and others,

Journal:

Cell metabolism (2020): 892--908

A Clickable APEX Probe for Proximity-Dependent Proteomic Profiling in Yeast

Authors:

Li, Yi and Tian, Caiping and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng

Journal:

Cell Chemical Biology (2020)

References

View all 73 references: Citation Explorer

Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation

Authors:

Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.

Journal:

Eur J Pharmacol (2006): 69

Mitochondrial Ca2+ uptake during simulated ischemia does not affect permeability transition pore opening upon simulated reperfusion

Authors:

Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.

Journal:

Cardiovasc Res (2006): 715

High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening

Authors:

O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.

Journal:

Arch Toxicol (2006): 580

NECA at reperfusion limits infarction and inhibits formation of the mitochondrial permeability transition pore by activating p70S6 kinase

Authors:

Forster K, Paul I, Solenkova N, Staudt A, Cohen MV, Downey JM, Felix SB, Krieg T.

Journal:

Basic Res Cardiol (2006): 319

Fatty acid ethyl esters cause pancreatic calcium toxicity via inositol trisphosphate receptors and loss of ATP synthesis

Authors:

Criddle DN, Murphy J, Fistetto G, Barrow S, Tepikin AV, Neoptolemos JP, Sutton R, Petersen OH.

Journal:

Gastroenterology (2006): 781

Physical properties

Molecular weight	500.93
Solvent	DMSO

Spectral properties

Excitation (nm)	552
Emission (nm)	574

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	115532-50-8

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 19, 2025