Name: TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0*
Brand: AAT Bioquest
SKU: 22220
Price: 113 USD
Availability: InStock

TMRE

Tetramethylrhodamine ethyl ester; CAS#: 115532-52-0

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.

<strong>CcO inhibition and PTM induce mitochondrial dysfunction. </strong>(A) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3 h treatment with NaN<sub>3</sub>. (B) Quantification of fluorescence intensity from (A). (C) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3 h treatment with KCN. (D) Quantification of fluorescence intensity from (C,E) Cellular ATP levels following 2 h treatment with NaN<sub>3</sub> and PTM. (F) Cellular ATP levels following 2 h treatment with KCN and PTM. Scale bar: 100 µm Data are mean + SEM from three independent experiments. Source: <strong>Cytochrome C oxidase Inhibition and Cold Plasma-derived Oxidants Synergize in Melanoma Cell Death Induction</strong> by Gandhirajan et al., <em>Scientific Reports</em>, Aug. 2018.

Protocol

SDS

Catalog	Size	Price	Quantity
22220	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	194.194 µL	970.968 µL	1.942 mL	9.71 mL	19.419 mL
5 mM	38.839 µL	194.194 µL	388.387 µL	1.942 mL	3.884 mL
10 mM	19.419 µL	97.097 µL	194.194 µL	970.968 µL	1.942 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	514.95 g/mol	=	mL	x	mM	=	mmol

Citations

View all 27 citations: Citation Explorer

COXFA4L3 enhances mitochondrial complex IV function to boost ATP synthesis and drive sperm motility

Authors:

Tokito, Reiji and Oishi, Kosei and Sugiyama, Tomoya and Fujisawa, Yusuke and Kuba, Fujino and Yoshida, Kaito and Yoshida, Kaoru and Yoshida, Manabu and Tanaka, Yoichiro and Amo, Taku and others,

Journal:

Mitochondrion (2025): 102082

Targeting mitochondrial metabolism by the mitotoxin bromoxib in leukemia and lymphoma cells

Authors:

Schmitt, Laura and Krings, Karina S and Wolsing, Andre and Buque, Xabier and Zimmermann, Marcel and Flores-Romero, Hector and Lenz, Thomas and Lechtenberg, Ilka and Peter, Christoph and Stork, Bj{\"o}rn and others,

Journal:

Cell Communication and Signaling: CCS (2024): 541

Palmitic Acid Exerts Anti-Tumorigenic Activities by Modulating Cellular Stress and Lipid Droplet Formation in Endometrial Cancer

Authors:

Zhao, Ziyi and Wang, Jiandong and Kong, Weimin and Newton, Meredith A and Burkett, Wesley C and Sun, Wenchuan and Buckingham, Lindsey and O’Donnell, Jillian and Suo, Hongyan and Deng, Boer and others,

Journal:

Biomolecules (2024): 601

Targeting mitochondrial metabolism by the mitotoxin bromoxib as a therapeutic approach for the treatment of leukemia and lymphoma

Authors:

Do the same chlorinated organophosphorus flame retardants that cause cytotoxicity and DNA damage share the same pathway?

Authors:

Yuan, Shengwu and Zhang, Hong and Wang, Shuhang and Jiang, Xia and Ma, Mei and Xu, Yiping and Han, Yingnan and Wang, Zijian

Journal:

Ecotoxicology and Environmental Safety (2024): 116158

References

View all 73 references: Citation Explorer

Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation

Authors:

Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.

Journal:

Eur J Pharmacol (2006): 69

Mitochondrial Ca2+ uptake during simulated ischemia does not affect permeability transition pore opening upon simulated reperfusion

Authors:

Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.

Journal:

Cardiovasc Res (2006): 715

High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening

Authors:

O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.

Journal:

Arch Toxicol (2006): 580

NECA at reperfusion limits infarction and inhibits formation of the mitochondrial permeability transition pore by activating p70S6 kinase

Authors:

Forster K, Paul I, Solenkova N, Staudt A, Cohen MV, Downey JM, Felix SB, Krieg T.

Journal:

Basic Res Cardiol (2006): 319

Fatty acid ethyl esters cause pancreatic calcium toxicity via inositol trisphosphate receptors and loss of ATP synthesis

Authors:

Criddle DN, Murphy J, Fistetto G, Barrow S, Tepikin AV, Neoptolemos JP, Sutton R, Petersen OH.

Journal:

Gastroenterology (2006): 781

Physical properties

Molecular weight	514.95
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.27
Correction factor (280 nm)	0.03
Excitation (nm)	552
Emission (nm)	574

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	115532-52-0

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Cell Viability & Proliferation
Brochure: Physiological Probes & Assay Kits

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Page updated on October 30, 2025