TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0*
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Physical properties
Molecular weight | 514.95 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.27 |
Correction Factor (280 nm) | 0.03 |
Excitation (nm) | 552 |
Emission (nm) | 574 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
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See also: Mitochondria, Rhodamines and Rhodamine Derivatives, Flow Cytometry Reagents, Mitochondrial Membrane Potential
CAS 115532-52-0 | Molecular weight 514.95 | Correction Factor (260 nm) 0.27 | Correction Factor (280 nm) 0.03 | Excitation (nm) 552 | Emission (nm) 574 |
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 194.194 µL | 970.968 µL | 1.942 mL | 9.71 mL | 19.419 mL |
5 mM | 38.839 µL | 194.194 µL | 388.387 µL | 1.942 mL | 3.884 mL |
10 mM | 19.419 µL | 97.097 µL | 194.194 µL | 970.968 µL | 1.942 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.27 |
Correction Factor (280 nm) | 0.03 |
Excitation (nm) | 552 |
Emission (nm) | 574 |
Images

Figure 1. CcO inhibition and PTM induce mitochondrial dysfunction. (A) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3 h treatment with NaN3. (B) Quantification of fluorescence intensity from (A). (C) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3 h treatment with KCN. (D) Quantification of fluorescence intensity from (C,E) Cellular ATP levels following 2 h treatment with NaN3 and PTM. (F) Cellular ATP levels following 2 h treatment with KCN and PTM. Scale bar: 100 µm Data are mean + SEM from three independent experiments. Source: Cytochrome C oxidase Inhibition and Cold Plasma-derived Oxidants Synergize in Melanoma Cell Death Induction by Gandhirajan et al., Scientific Reports, Aug. 2018.
Citations
View all 16 citations: Citation Explorer
Ipatasertib exhibits anti-tumorigenic effects and enhances sensitivity to paclitaxel in endometrial cancer in vitro and in vivo
Authors: O'Donnell, Jillian and Zhao, Ziyi and Buckingham, Lindsey and Hao, Tianran and Suo, Hongyan and Zhang, Xin and Fan, Yali and John, Catherine and Deng, Boer and Shen, Xiaochang and others,
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Journal: International Journal of Oncology (2023): 1--14
BNIP3 (BCL2 interacting protein 3) regulates pluripotency by modulating mitochondrial homeostasis via mitophagy
Authors: Liu, Kun and Zhao, Qian and Sun, Hongyan and Liu, Lei and Wang, Chaoqun and Li, Zheng and Xu, Youqing and Wang, Liang and Zhang, Lin and Zhang, Honghai and others,
Journal: Cell death \& disease (2022): 1--8
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Journal: Cell death \& disease (2022): 1--8
The mycotoxin viriditoxin induces leukemia-and lymphoma-specific apoptosis by targeting mitochondrial metabolism
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Journal: Cell Death \& Disease (2022): 1--17
Authors: Stuhldreier, Fabian and Schmitt, Laura and Lenz, Thomas and Hinxlage, Ilka and Zimmermann, Marcel and Wollnitzke, Philipp and Schliehe-Diecks, Julian and Liu, Yang and J{\"a}ger, Paul and Geyh, Stefanie and others,
Journal: Cell Death \& Disease (2022): 1--17
Metformin dampens cisplatin cytotoxicity on leukemia cells after incorporation into cubosomal nanoformulation
Authors: Saber, Mona M and Al-mahallawi, Abdulaziz M and Stork, Bj{\"o}rn
Journal: Biomedicine \& Pharmacotherapy (2021): 112140
Authors: Saber, Mona M and Al-mahallawi, Abdulaziz M and Stork, Bj{\"o}rn
Journal: Biomedicine \& Pharmacotherapy (2021): 112140
The amino acid metabolism is essential for evading physical plasma-induced tumour cell death
Authors: Gandhirajan, Rajesh Kumar and Meyer, Dorothee and Sagwal, Sanjeev Kumar and Weltmann, Klaus-Dieter and von Woedtke, Thomas and Bekeschus, Sander
Journal: British journal of cancer (2021): 1854--1863
Authors: Gandhirajan, Rajesh Kumar and Meyer, Dorothee and Sagwal, Sanjeev Kumar and Weltmann, Klaus-Dieter and von Woedtke, Thomas and Bekeschus, Sander
Journal: British journal of cancer (2021): 1854--1863
Cytotoxicity, mitochondrial impairment, DNA damage and associated mechanisms induced by tris (1, 3-dichloro-2-propyl) phosphate and tris (2-butoxyethyl) phosphate in A549 cells
Authors: Yuan, Shengwu and Ma, Mei and Zhu, Xiaoshan and Han, Yingnan and Rao, Kaifeng and Wang, Zijian
Journal: Science of The Total Environment (2021): 147668
Authors: Yuan, Shengwu and Ma, Mei and Zhu, Xiaoshan and Han, Yingnan and Rao, Kaifeng and Wang, Zijian
Journal: Science of The Total Environment (2021): 147668
PINK1-mediated mitophagy maintains pluripotency through optineurin
Authors: Wang, Chaoqun and Liu, Kun and Cao, Jiani and Wang, Liang and Zhao, Qian and Li, Zheng and Zhang, Honghai and Chen, Quan and Zhao, Tongbiao
Journal: Cell Proliferation (2021): e13034
Authors: Wang, Chaoqun and Liu, Kun and Cao, Jiani and Wang, Liang and Zhao, Qian and Li, Zheng and Zhang, Honghai and Chen, Quan and Zhao, Tongbiao
Journal: Cell Proliferation (2021): e13034
Ronin governs the metabolic capacity of the embryonic lineage for post-implantation development
Authors: Salewskij, Kirill and Gross-Thebing, Theresa and Ing-Simmons, Elizabeth and Duethorn, Binyamin and Rieger, Bettina and Fan, Rui and Chen, Rui and Govindasamy, Niraimathi and Brinkmann, Heike and Kremer, Ludmila and others,
Journal: EMBO reports (2021): e53048
Authors: Salewskij, Kirill and Gross-Thebing, Theresa and Ing-Simmons, Elizabeth and Duethorn, Binyamin and Rieger, Bettina and Fan, Rui and Chen, Rui and Govindasamy, Niraimathi and Brinkmann, Heike and Kremer, Ludmila and others,
Journal: EMBO reports (2021): e53048
In vitro oxidative stress, mitochondrial impairment and G1 phase cell cycle arrest induced by alkyl-phosphorus-containing flame retardants
Authors: Yuan, Shengwu and Zhu, Kongrui and Ma, Mei and Zhu, Xiaoshan and Rao, Kaifeng and Wang, Zijian
Journal: Chemosphere (2020): 126026
Authors: Yuan, Shengwu and Zhu, Kongrui and Ma, Mei and Zhu, Xiaoshan and Rao, Kaifeng and Wang, Zijian
Journal: Chemosphere (2020): 126026
Mitochondrial survivin reduces oxidative phosphorylation in cancer cells by inhibiting mitophagy
Authors: Townley, Amelia R and Wheatley, Sally P
Journal: Journal of cell science (2020): jcs247379
Authors: Townley, Amelia R and Wheatley, Sally P
Journal: Journal of cell science (2020): jcs247379
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