X
Navigation Menu
Products
Services
Tools
Resources
logo
AAT Bioquest

 
search
logo
AAT Bioquest
Contact us
Company
Telephone:1-408-733-1055
Fax:1-408-733-1304
Hours:Monday to Friday
8:30 - 17:30 PST (GMT-8)
Location:5775 W Las Positas Blvd.
Pleasanton, CA, 94588
USA

Email us
Sales:sales@aatbio.com
Technical Support:support@aatbio.com
Website:websupport@aatbio.com
General Inquiries:info@aatbio.com
Order info
United States of America
Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

International
Click here to see all available distributors
Products
Services
Tools
Resources
 
search
calculatorCalculator

TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0*

Documents
pdfCertificate of origin
pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Related catalogs
Mitochondria
Rhodamines and Rhodamine Derivatives
Flow Cytometry Reagents
Mitochondrial Membrane Potential
Related products
Rhodamine 700 *CAS 63561-42-2*
Rhodamine 800 *CAS 137993-41-0*
Rhodamine B *CAS 81-88-9*
Rhodamine 6G *CAS 989-38-8*
Sulforhodamine 101, sodium salt
Sulforhodamine B *CAS 3520-42-1*
Sulforhodamine G *CAS 5873-16-5*
Rhodamine 101 Inner Salt *CAS 116450-56-7*
Rhodamine 101 Chloride Salt *CAS 64339-18-0*
Sulforhodamine 101 sodium salt *CAS 465544-24-5*
Rhodamine 110 *CAS 13558-31-1*
5-CR110 [5-Carboxyrhodamine 110] *Single isomer*
6-CR110 [6-Carboxyrhodamine 110] *Single isomer*
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*
6-CR6G [6-Carboxyrhodamine 6G] *Single isomer*
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
5(6)-Carboxyrhodamine 6G cadaverine
5(6)-Carboxyrhodamine 6G ethylenediamine
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6*
5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4*
6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5*
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*
5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*
6-ROX [6-Carboxy-X-rhodamine] *CAS 194785-18-7*
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers*
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7*
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*
5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2*
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5*
6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4*
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5*
Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6*
Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*
SRB-VAD-FMK [Sulforhodamine B-VAD-FMK]
Dihydrorhodamine 123 *CAS 109244-58-8*
Tetramethylrhodamine-dUTP *1 mM in TE Buffer (pH 7.5)*
Rhodamine 123 *CAS 62669-70-9*
Rhodamine B, hexyl ester, perchlorate
TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*
Phalloidin-Tetramethylrhodamine Conjugate
5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G]
6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]
5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]
6-dR110 [6-Carboxy-4,7-dichlororhodamine 110]
Bis-L-Aspartic acid Beta-rhodamine 110
Bis-Arg-rhodamine 110
Sulforhodamine 101 *CAS 60311-02-6*
5-Carboxyrhodamine 6G maleimide
Rhodamine aldehyde [5-TAMRA aldehyde]
Show More (47)
FAQ
Why is determining cell viability important?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Annexin V
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
Calcein
AssayWise
Selective Probes for Studying Mitochondrial Functionality
Fluorescent Tools for Studying Mitochondrial Morphology and Function
Comprehensive Guide to Cell Health Assays in Flow Cytometry: A Multidimensional Exploration
Glycerol 3-Phosphate Assays
Nucleic Acid Quantification
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.
<strong>CcO inhibition and PTM induce mitochondrial dysfunction. </strong>(A) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with NaN<sub>3</sub>. (B) Quantification of fluorescence intensity from (A). (C) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with KCN. (D) Quantification of fluorescence intensity from (C,E) Cellular ATP levels following 2&thinsp;h treatment with NaN<sub>3</sub> and PTM. (F) Cellular ATP levels following 2&thinsp;h treatment with KCN and PTM. Scale bar: 100&thinsp;&micro;m Data are mean&thinsp;+&thinsp;SEM from three independent experiments. Source: <strong>Cytochrome C oxidase Inhibition and Cold Plasma-derived Oxidants Synergize in Melanoma Cell Death Induction</strong> by Gandhirajan et al., <em>Scientific Reports</em>, Aug. 2018.
<strong>CcO inhibition and PTM induce mitochondrial dysfunction. </strong>(A) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with NaN<sub>3</sub>. (B) Quantification of fluorescence intensity from (A). (C) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with KCN. (D) Quantification of fluorescence intensity from (C,E) Cellular ATP levels following 2&thinsp;h treatment with NaN<sub>3</sub> and PTM. (F) Cellular ATP levels following 2&thinsp;h treatment with KCN and PTM. Scale bar: 100&thinsp;&micro;m Data are mean&thinsp;+&thinsp;SEM from three independent experiments. Source: <strong>Cytochrome C oxidase Inhibition and Cold Plasma-derived Oxidants Synergize in Melanoma Cell Death Induction</strong> by Gandhirajan et al., <em>Scientific Reports</em>, Aug. 2018.
<strong>CcO inhibition and PTM induce mitochondrial dysfunction. </strong>(A) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with NaN<sub>3</sub>. (B) Quantification of fluorescence intensity from (A). (C) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with KCN. (D) Quantification of fluorescence intensity from (C,E) Cellular ATP levels following 2&thinsp;h treatment with NaN<sub>3</sub> and PTM. (F) Cellular ATP levels following 2&thinsp;h treatment with KCN and PTM. Scale bar: 100&thinsp;&micro;m Data are mean&thinsp;+&thinsp;SEM from three independent experiments. Source: <strong>Cytochrome C oxidase Inhibition and Cold Plasma-derived Oxidants Synergize in Melanoma Cell Death Induction</strong> by Gandhirajan et al., <em>Scientific Reports</em>, Aug. 2018.
Gallery Image 2
Ordering information
Price
Unit size
Catalog Number22220
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight514.95
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.27
Correction Factor (280 nm)0.03
Excitation (nm)552
Emission (nm)574
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS115532-52-0
Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM194.194 µL970.968 µL1.942 mL9.71 mL19.419 mL
5 mM38.839 µL194.194 µL388.387 µL1.942 mL3.884 mL
10 mM19.419 µL97.097 µL194.194 µL970.968 µL1.942 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=
Spectrum
Citations
View all 23 citations: Citation Explorer
Palmitic Acid Exerts Anti-Tumorigenic Activities by Modulating Cellular Stress and Lipid Droplet Formation in Endometrial Cancer
Authors: Zhao, Ziyi and Wang, Jiandong and Kong, Weimin and Newton, Meredith A and Burkett, Wesley C and Sun, Wenchuan and Buckingham, Lindsey and O’Donnell, Jillian and Suo, Hongyan and Deng, Boer and others,
Journal: Biomolecules (2024): 601
Targeting mitochondrial metabolism by the mitotoxin bromoxib as a therapeutic approach for the treatment of leukemia and lymphoma
Authors: Schmitt, Laura and Krings, Karina S and Wolsing, Andre and Buque, Xabier and Zimmermann, Marcel and Flores-Romero, Hector and Lenz, Thomas and Lechtenberg, Ilka and Peter, Christoph and Stork, Bj{\"o}rn and others,
Journal: (2024)
Do the same chlorinated organophosphorus flame retardants that cause cytotoxicity and DNA damage share the same pathway?
Authors: Yuan, Shengwu and Zhang, Hong and Wang, Shuhang and Jiang, Xia and Ma, Mei and Xu, Yiping and Han, Yingnan and Wang, Zijian
Journal: Ecotoxicology and Environmental Safety (2024): 116158
Linoleic acid exhibits anti-proliferative and anti-invasive activities in endometrial cancer cells and a transgenic model of endometrial cancer
Authors: Qiu, Jianqing and Zhao, Ziyi and Suo, Hongyan and Paraghamian, Sarah E and Hawkins, Gabrielle M and Sun, Wenchuan and Zhang, Xin and Hao, Tianran and Deng, Beor and Shen, Xiaochang and others,
Journal: Cancer Biology \& Therapy (2024): 2325130
Novel meriolin derivatives activate the mitochondrial apoptosis pathway in the presence of antiapoptotic Bcl-2
Authors: Schmitt, Laura and Lechtenberg, Ilka and Drie{\ss}en, Daniel and Flores-Romero, Hector and Skowron, Margaretha A and Sekeres, Marlena and Hoppe, Julia and Krings, Karina S and Llewellyn, Tanya R and Peter, Christoph and others,
Journal: Cell Death Discovery (2024): 125
References
View all 73 references: Citation Explorer
CRYAB and HSPB2 deficiency increases myocyte mitochondrial permeability transition and mitochondrial calcium uptake
Authors: Kadono T, Zhang XQ, Srinivasan S, Ishida H, Barry WH, Benjamin IJ.
Journal: J Mol Cell Cardiol (2006): 783
Mitochondrial Ca2+ uptake during simulated ischemia does not affect permeability transition pore opening upon simulated reperfusion
Authors: Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.
Journal: Cardiovasc Res (2006): 715
High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening
Authors: O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.
Journal: Arch Toxicol (2006): 580
Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation
Authors: Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.
Journal: Eur J Pharmacol (2006): 69
NECA at reperfusion limits infarction and inhibits formation of the mitochondrial permeability transition pore by activating p70S6 kinase
Authors: Forster K, Paul I, Solenkova N, Staudt A, Cohen MV, Downey JM, Felix SB, Krieg T.
Journal: Basic Res Cardiol (2006): 319
Location: Home / Products / TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0*
AboutPrivacyTerms of UseTerms of SalesDistributors
Copyright © 2024 AAT Bioquest, Inc. All Rights Reserved.