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TMRE
Tetramethylrhodamine ethyl ester; CAS#: 115532-52-0
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.
<strong>CcO inhibition and PTM induce mitochondrial dysfunction. </strong>(A) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with NaN<sub>3</sub>. (B) Quantification of fluorescence intensity from (A). (C) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with KCN. (D) Quantification of fluorescence intensity from (C,E) Cellular ATP levels following 2&thinsp;h treatment with NaN<sub>3</sub> and PTM. (F) Cellular ATP levels following 2&thinsp;h treatment with KCN and PTM. Scale bar: 100&thinsp;&micro;m Data are mean&thinsp;+&thinsp;SEM from three independent experiments. Source: <strong>Cytochrome C oxidase Inhibition and Cold Plasma-derived Oxidants Synergize in Melanoma Cell Death Induction</strong> by Gandhirajan et al., <em>Scientific Reports</em>, Aug. 2018.
<strong>CcO inhibition and PTM induce mitochondrial dysfunction. </strong>(A) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with NaN<sub>3</sub>. (B) Quantification of fluorescence intensity from (A). (C) Mitochondrial membrane potential enumerated by TMRE dye uptake and cellular superoxide levels by nuclear DHE staining in B16F10 cells following 3&thinsp;h treatment with KCN. (D) Quantification of fluorescence intensity from (C,E) Cellular ATP levels following 2&thinsp;h treatment with NaN<sub>3</sub> and PTM. (F) Cellular ATP levels following 2&thinsp;h treatment with KCN and PTM. Scale bar: 100&thinsp;&micro;m Data are mean&thinsp;+&thinsp;SEM from three independent experiments. Source: <strong>Cytochrome C oxidase Inhibition and Cold Plasma-derived Oxidants Synergize in Melanoma Cell Death Induction</strong> by Gandhirajan et al., <em>Scientific Reports</em>, Aug. 2018.
CatalogSize
Price
Quantity
2222025 mg
Price
 
Physical properties

Molecular weight514.95
SolventDMSO
Spectral properties

Correction factor (260 nm)0.27
Correction factor (280 nm)0.03
Excitation (nm)552
Emission (nm)574
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS115532-52-0
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Page updated on October 8, 2025