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Rhodamine B, hexyl ester, perchlorate

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. This particular rhodamine ester stains mitochondria orange in fluorescence. Its spectral properties are similar to those of TRITC, making the use of Rhodamine B hexyl ester quite convenient.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B, hexyl ester, perchlorate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM159.446 µL797.232 µL1.594 mL7.972 mL15.945 mL
5 mM31.889 µL159.446 µL318.893 µL1.594 mL3.189 mL
10 mM15.945 µL79.723 µL159.446 µL797.232 µL1.594 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Citations

View all 1 citations: Citation Explorer
Mechanism of transdermal permeation promotion of lipophilic drugs by ethosomes
Authors: Yang, Li and Wu, Lifang and Wu, Dongze and Shi, Deshun and Wang, Tai and Zhu, Xiaoliang
Journal: International journal of nanomedicine (2017): 3357

References

View all 29 references: Citation Explorer
Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Nature and significance of the electron-dense bodies of the endospores of rhinosporidium seeberi: their reactions with MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) and TMRE (tetramethyl-rhodamine ethyl ester)
Authors: Arseculeratne SN, Atapattu DN, Wickramaratne K.
Journal: Med Mycol (2005): 261
Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430
Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767
Cadmium-induced mitochondrial membrane-potential dissipation does not necessarily require cytosolic oxidative stress: studies using rhodamine-123 fluorescence unquenching
Authors: Bolduc JS, Denizeau F, Jumarie C.
Journal: Toxicol Sci (2004): 299
Page updated on October 4, 2024

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Catalog Number22211
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Physical properties

Molecular weight

627.17

Solvent

DMSO

Spectral properties

Absorbance (nm)

541

Extinction coefficient (cm -1 M -1)

1060001

Excitation (nm)

546

Emission (nm)

567

Quantum yield

0.71, 0.432

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200