Rhodamine B, hexyl ester, perchlorate

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Physical properties

Molecular weight	627.17
Solvent	DMSO

Spectral properties

Absorbance (nm)	541
Extinction coefficient (cm ^-1 M ^-1)	106000¹
Excitation (nm)	546
Emission (nm)	567
Quantum yield	0.7¹, 0.43²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

627.17

Absorbance (nm)

541

Extinction coefficient (cm ^-1 M ^-1)

106000¹

Excitation (nm)

546

Emission (nm)

567

Quantum yield

0.7¹, 0.43²

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. This particular rhodamine ester stains mitochondria orange in fluorescence. Its spectral properties are similar to those of TRITC, making the use of Rhodamine B hexyl ester quite convenient.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B, hexyl ester, perchlorate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	159.446 µL	797.232 µL	1.594 mL	7.972 mL	15.945 mL
5 mM	31.889 µL	159.446 µL	318.893 µL	1.594 mL	3.189 mL
10 mM	15.945 µL	79.723 µL	159.446 µL	797.232 µL	1.594 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	541
Extinction coefficient (cm ^-1 M ^-1)	106000¹
Excitation (nm)	546
Emission (nm)	567
Quantum yield	0.7¹, 0.43²

Images

Figure 1. Chemical structure for Rhodamine B, hexyl ester, perchlorate

Citations

View all 1 citations: Citation Explorer

Mechanism of transdermal permeation promotion of lipophilic drugs by ethosomes
Authors: Yang, Li and Wu, Lifang and Wu, Dongze and Shi, Deshun and Wang, Tai and Zhu, Xiaoliang
Journal: International journal of nanomedicine (2017): 3357

References

View all 29 references: Citation Explorer

Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009

Nature and significance of the electron-dense bodies of the endospores of rhinosporidium seeberi: their reactions with MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) and TMRE (tetramethyl-rhodamine ethyl ester)
Authors: Arseculeratne SN, Atapattu DN, Wickramaratne K.
Journal: Med Mycol (2005): 261

Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430

Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767

Cadmium-induced mitochondrial membrane-potential dissipation does not necessarily require cytosolic oxidative stress: studies using rhodamine-123 fluorescence unquenching
Authors: Bolduc JS, Denizeau F, Jumarie C.
Journal: Toxicol Sci (2004): 299

Interference of calmidazolium with measurement of mitochondrial membrane potential using the tetraphenylphosphonium electrode or the fluorescent probe rhodamine 123
Authors: Saris NE, Teplova VV, Odinokova IV, Azarashvily TS.
Journal: Anal Biochem (2004): 109

Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells
Authors: Schneckenburger H, Stock K, Lyttek M, Strauss WS, Sailer R.
Journal: Photochem Photobiol Sci (2004): 127

Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis
Authors: Baracca A, Sgarbi G, Solaini G, Lenaz G.
Journal: Biochim Biophys Acta (2003): 137

Rhodamine B as a mitochondrial probe for measurement and monitoring of mitochondrial membrane potential in drug-sensitive and -resistant cells
Authors: Reungpatthanaphong P, Dechsupa S, Meesungnoen J, Loetchutinat C, Mankhetkorn S.
Journal: J Biochem Biophys Methods (2003): 1

Microfluorometric study of oxygen dependence of (1"-pyrene butyl)-2-rhodamine ester probe in mitochondria of living cells
Authors: Ribou AC, Vigo J, Kohen E, Salmon JM.
Journal: J Photochem Photobiol B (2003): 107