Name: Rhodamine B, hexyl ester, perchlorate
Brand: AAT Bioquest
SKU: 22211
Price: 90 USD
Availability: InStock

Rhodamine B, hexyl ester, perchlorate

Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. This particular rhodamine ester stains mitochondria orange in fluorescence. Its spectral properties are similar to those of TRITC, making the use of Rhodamine B hexyl ester quite convenient.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22211	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B, hexyl ester, perchlorate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	159.446 µL	797.232 µL	1.594 mL	7.972 mL	15.945 mL
5 mM	31.889 µL	159.446 µL	318.893 µL	1.594 mL	3.189 mL
10 mM	15.945 µL	79.723 µL	159.446 µL	797.232 µL	1.594 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	627.17 g/mol	=	mL	x	mM	=	mmol

References

View all 29 references: Citation Explorer

Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts

Authors:

Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.

Journal:

J Biomed Opt (2006): 14009

Nature and significance of the electron-dense bodies of the endospores of rhinosporidium seeberi: their reactions with MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) and TMRE (tetramethyl-rhodamine ethyl ester)

Authors:

Arseculeratne SN, Atapattu DN, Wickramaratne K.

Journal:

Med Mycol (2005): 261

Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123

Authors:

Lacerda SH, Abraham B, Stringfellow TC, Indig GL.

Journal:

Photochem Photobiol (2005): 1430

Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye

Authors:

Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.

Journal:

Biol Reprod (2005): 767

Cadmium-induced mitochondrial membrane-potential dissipation does not necessarily require cytosolic oxidative stress: studies using rhodamine-123 fluorescence unquenching

Authors:

Bolduc JS, Denizeau F, Jumarie C.

Journal:

Toxicol Sci (2004): 299

Physical properties

Molecular weight	627.17
Solvent	DMSO

Spectral properties

Absorbance (nm)	541
Extinction coefficient (cm ^-1 M ^-1)	106000 ¹
Excitation (nm)	546
Emission (nm)	567
Quantum yield	0.7 ¹ , 0.43 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 26, 2025