Rhodamine B, hexyl ester, perchlorate
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 627.17 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 541 |
Extinction coefficient (cm -1 M -1) | 1060001 |
Excitation (nm) | 546 |
Emission (nm) | 567 |
Quantum yield | 0.71, 0.432 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
See also: Cell Structures and Organelles, Mitochondria, Chemical Reagents, Classic Dyes, Rhodamines and Rhodamine Derivatives
Molecular weight 627.17 | Absorbance (nm) 541 | Extinction coefficient (cm -1 M -1) 1060001 | Excitation (nm) 546 | Emission (nm) 567 | Quantum yield 0.71, 0.432 |
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. This particular rhodamine ester stains mitochondria orange in fluorescence. Its spectral properties are similar to those of TRITC, making the use of Rhodamine B hexyl ester quite convenient.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B, hexyl ester, perchlorate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 159.446 µL | 797.232 µL | 1.594 mL | 7.972 mL | 15.945 mL |
5 mM | 31.889 µL | 159.446 µL | 318.893 µL | 1.594 mL | 3.189 mL |
10 mM | 15.945 µL | 79.723 µL | 159.446 µL | 797.232 µL | 1.594 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 541 |
Extinction coefficient (cm -1 M -1) | 1060001 |
Excitation (nm) | 546 |
Emission (nm) | 567 |
Quantum yield | 0.71, 0.432 |
Citations
View all 1 citations: Citation Explorer
Mechanism of transdermal permeation promotion of lipophilic drugs by ethosomes
Authors: Yang, Li and Wu, Lifang and Wu, Dongze and Shi, Deshun and Wang, Tai and Zhu, Xiaoliang
Journal: International journal of nanomedicine (2017): 3357
Authors: Yang, Li and Wu, Lifang and Wu, Dongze and Shi, Deshun and Wang, Tai and Zhu, Xiaoliang
Journal: International journal of nanomedicine (2017): 3357
References
View all 29 references: Citation Explorer
Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Nature and significance of the electron-dense bodies of the endospores of rhinosporidium seeberi: their reactions with MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) and TMRE (tetramethyl-rhodamine ethyl ester)
Authors: Arseculeratne SN, Atapattu DN, Wickramaratne K.
Journal: Med Mycol (2005): 261
Authors: Arseculeratne SN, Atapattu DN, Wickramaratne K.
Journal: Med Mycol (2005): 261
Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430
Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767
Cadmium-induced mitochondrial membrane-potential dissipation does not necessarily require cytosolic oxidative stress: studies using rhodamine-123 fluorescence unquenching
Authors: Bolduc JS, Denizeau F, Jumarie C.
Journal: Toxicol Sci (2004): 299
Authors: Bolduc JS, Denizeau F, Jumarie C.
Journal: Toxicol Sci (2004): 299
Interference of calmidazolium with measurement of mitochondrial membrane potential using the tetraphenylphosphonium electrode or the fluorescent probe rhodamine 123
Authors: Saris NE, Teplova VV, Odinokova IV, Azarashvily TS.
Journal: Anal Biochem (2004): 109
Authors: Saris NE, Teplova VV, Odinokova IV, Azarashvily TS.
Journal: Anal Biochem (2004): 109
Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells
Authors: Schneckenburger H, Stock K, Lyttek M, Strauss WS, Sailer R.
Journal: Photochem Photobiol Sci (2004): 127
Authors: Schneckenburger H, Stock K, Lyttek M, Strauss WS, Sailer R.
Journal: Photochem Photobiol Sci (2004): 127
Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis
Authors: Baracca A, Sgarbi G, Solaini G, Lenaz G.
Journal: Biochim Biophys Acta (2003): 137
Authors: Baracca A, Sgarbi G, Solaini G, Lenaz G.
Journal: Biochim Biophys Acta (2003): 137
Rhodamine B as a mitochondrial probe for measurement and monitoring of mitochondrial membrane potential in drug-sensitive and -resistant cells
Authors: Reungpatthanaphong P, Dechsupa S, Meesungnoen J, Loetchutinat C, Mankhetkorn S.
Journal: J Biochem Biophys Methods (2003): 1
Authors: Reungpatthanaphong P, Dechsupa S, Meesungnoen J, Loetchutinat C, Mankhetkorn S.
Journal: J Biochem Biophys Methods (2003): 1
Microfluorometric study of oxygen dependence of (1"-pyrene butyl)-2-rhodamine ester probe in mitochondria of living cells
Authors: Ribou AC, Vigo J, Kohen E, Salmon JM.
Journal: J Photochem Photobiol B (2003): 107
Authors: Ribou AC, Vigo J, Kohen E, Salmon JM.
Journal: J Photochem Photobiol B (2003): 107