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Rhodamine B, hexyl ester, perchlorate

Chemical structure for Rhodamine B, hexyl ester, perchlorate
Chemical structure for Rhodamine B, hexyl ester, perchlorate
Ordering information
Price ()
Catalog Number22211
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight627.17
SolventDMSO
Spectral properties
Absorbance (nm)541
Extinction coefficient (cm -1 M -1)1060001
Excitation (nm)546
Emission (nm)568
Quantum yield0.311
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Show More (57)

OverviewpdfSDSpdfProtocol


Molecular weight
627.17
Absorbance (nm)
541
Extinction coefficient (cm -1 M -1)
1060001
Excitation (nm)
546
Emission (nm)
568
Quantum yield
0.311
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. This particular rhodamine ester stains mitochondria orange in fluorescence. Its spectral properties are similar to those of TRITC, making the use of Rhodamine B hexyl ester quite convenient.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B, hexyl ester, perchlorate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM159.446 µL797.232 µL1.594 mL7.972 mL15.945 mL
5 mM31.889 µL159.446 µL318.893 µL1.594 mL3.189 mL
10 mM15.945 µL79.723 µL159.446 µL797.232 µL1.594 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)541
Extinction coefficient (cm -1 M -1)1060001
Excitation (nm)546
Emission (nm)568
Quantum yield0.311

Citations


View all 1 citations: Citation Explorer
Mechanism of transdermal permeation promotion of lipophilic drugs by ethosomes
Authors: Yang, Li and Wu, Lifang and Wu, Dongze and Shi, Deshun and Wang, Tai and Zhu, Xiaoliang
Journal: International journal of nanomedicine (2017): 3357

References


View all 29 references: Citation Explorer
Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Nature and significance of the electron-dense bodies of the endospores of rhinosporidium seeberi: their reactions with MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) and TMRE (tetramethyl-rhodamine ethyl ester)
Authors: Arseculeratne SN, Atapattu DN, Wickramaratne K.
Journal: Med Mycol (2005): 261
Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123
Authors: Lacerda SH, Abraham B, Stringfellow TC, Indig GL.
Journal: Photochem Photobiol (2005): 1430
Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye
Authors: Lo KC, Brugh VM, 3rd, Parker M, Lamb DJ.
Journal: Biol Reprod (2005): 767
Cadmium-induced mitochondrial membrane-potential dissipation does not necessarily require cytosolic oxidative stress: studies using rhodamine-123 fluorescence unquenching
Authors: Bolduc JS, Denizeau F, Jumarie C.
Journal: Toxicol Sci (2004): 299
Interference of calmidazolium with measurement of mitochondrial membrane potential using the tetraphenylphosphonium electrode or the fluorescent probe rhodamine 123
Authors: Saris NE, Teplova VV, Odinokova IV, Azarashvily TS.
Journal: Anal Biochem (2004): 109
Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells
Authors: Schneckenburger H, Stock K, Lyttek M, Strauss WS, Sailer R.
Journal: Photochem Photobiol Sci (2004): 127
Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis
Authors: Baracca A, Sgarbi G, Solaini G, Lenaz G.
Journal: Biochim Biophys Acta (2003): 137
Rhodamine B as a mitochondrial probe for measurement and monitoring of mitochondrial membrane potential in drug-sensitive and -resistant cells
Authors: Reungpatthanaphong P, Dechsupa S, Meesungnoen J, Loetchutinat C, Mankhetkorn S.
Journal: J Biochem Biophys Methods (2003): 1
Microfluorometric study of oxygen dependence of (1"-pyrene butyl)-2-rhodamine ester probe in mitochondria of living cells
Authors: Ribou AC, Vigo J, Kohen E, Salmon JM.
Journal: J Photochem Photobiol B (2003): 107