Dihydrorhodamine 123 *CAS 109244-58-8*
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Physical properties
Molecular weight | 346.38 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 497 |
Extinction coefficient (cm -1 M -1) | 85200 |
Excitation (nm) | 508 |
Emission (nm) | 528 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
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See also: Rhodamines and Rhodamine Derivatives
CAS 109244-58-8 | Molecular weight 346.38 | Absorbance (nm) 497 | Extinction coefficient (cm -1 M -1) 85200 | Excitation (nm) 508 | Emission (nm) 528 |
Dihydrorhodamine 123 is by far the most-used probe for measurement of intracellular H2O2. DHR 123 is oxidized directly to rhodamine 123, which is excitable at 488 and emits at 515 nm in the same emission range as FITC. It is widely used in human neutrophils, human eosinophils, HL60 cells, rat mast cells, guinea pig neutrophils, cultured chondrocytes, rat brain, rat renal proximal tubular cells, mesangial cells and L929 cells. In combination with other fluorescent reagents (such as surface receptor analysis by fluorescent antibodies, cell viability using propidium iodide, and calcium indicators) this probe can be used for multiplex measurements.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Dihydrorhodamine 123 *CAS 109244-58-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 288.7 µL | 1.444 mL | 2.887 mL | 14.435 mL | 28.87 mL |
5 mM | 57.74 µL | 288.7 µL | 577.401 µL | 2.887 mL | 5.774 mL |
10 mM | 28.87 µL | 144.35 µL | 288.7 µL | 1.444 mL | 2.887 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 497 |
Extinction coefficient (cm -1 M -1) | 85200 |
Excitation (nm) | 508 |
Emission (nm) | 528 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 |
Citations
View all 7 citations: Citation Explorer
Variations in reactive oxygen species generation by urban airborne particulate matter in lung epithelial cells—impact of inorganic fraction
Authors: Mazuryk, Olga and Stochel, Grazyna and Brindell, Ma{\l}gorzata
Journal: Frontiers in chemistry (2020): 581752
Authors: Mazuryk, Olga and Stochel, Grazyna and Brindell, Ma{\l}gorzata
Journal: Frontiers in chemistry (2020): 581752
Direct macromolecular drug delivery to cerebral ischemia area using neutrophil-mediated nanoparticles
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin
Journal: Theranostics (2017): 3260
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin
Journal: Theranostics (2017): 3260
Liver necrosis and lethal systemic inflammation in a murine model of Rickettsia typhi infection: role of neutrophils, macrophages and NK cells
Authors: Papp, Stefanie and Moderzynski, Kristin and Rauch, Jessica and Heine, Liza and Kuehl, Svenja and Richardt, Ulricke and Mueller, Heidelinde and Fleischer, Bernhard and Osterloh, Anke
Journal: PLoS Negl Trop Dis (2016): e0004935
Authors: Papp, Stefanie and Moderzynski, Kristin and Rauch, Jessica and Heine, Liza and Kuehl, Svenja and Richardt, Ulricke and Mueller, Heidelinde and Fleischer, Bernhard and Osterloh, Anke
Journal: PLoS Negl Trop Dis (2016): e0004935
Charakterisierung angeborener Immunmechanismen und immunpathologischer Effekte in der Rickettsia typhi-Infektion (Wolbach und Todd, 1920) in der Maus (Mus musculus, Linnaeus, 1758)
Authors: Papp, Stefanie
Journal: (2015)
Authors: Papp, Stefanie
Journal: (2015)
Charakterisierung der immunmodulatorischen Funktion von Siglec-E (Sialins{\"a}ure-bindendes-Ig-{\"a}hnliches-Lektin-E) im Modell der experimentellen Chagas-Krankheit
Authors: Heins, Anja
Journal: (2013)
Authors: Heins, Anja
Journal: (2013)
Bicarbonate plays a critical role in the generation of cytotoxicity during SIN-1 decomposition in culture medium
Authors: Shirai, Kyo and Okada, Tatsumi and Konishi, Kanako and Murata, Hiroshi and Akashi, Soichiro and Sugawara, Fumio and Watanabe, Nobuo and Arai, Takao
Journal: Oxidative medicine and cellular longevity (2012)
Authors: Shirai, Kyo and Okada, Tatsumi and Konishi, Kanako and Murata, Hiroshi and Akashi, Soichiro and Sugawara, Fumio and Watanabe, Nobuo and Arai, Takao
Journal: Oxidative medicine and cellular longevity (2012)
Direct Macromolecular Drug Delivery to Cerebral Ischemia Area using Neutrophil-Mediated Nanoparticles
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin
References
View all 20 references: Citation Explorer
Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD
Authors: Mauch L, Lun A, O'Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler J.
Journal: Clin Chem (2007): 890
Authors: Mauch L, Lun A, O'Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler J.
Journal: Clin Chem (2007): 890
Reactivity of 2',7'-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals
Authors: Wrona M, Patel K, Wardman P.
Journal: Free Radic Biol Med (2005): 262
Authors: Wrona M, Patel K, Wardman P.
Journal: Free Radic Biol Med (2005): 262
Fluorescence measurements of steady state peroxynitrite production upon SIN-1 decomposition: NADH versus dihydrodichlorofluorescein and dihydrorhodamine 123
Authors: Martin-Romero FJ, Gutierrez-Martin Y, Henao F, Gutierrez-Merino C.
Journal: J Fluoresc (2004): 17
Authors: Martin-Romero FJ, Gutierrez-Martin Y, Henao F, Gutierrez-Merino C.
Journal: J Fluoresc (2004): 17
Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay
Authors: Jirapongsananuruk O, Malech HL, Kuhns DB, Niemela JE, Brown MR, Anderson-Cohen M, Fleisher TA.
Journal: J Allergy Clin Immunol (2003): 374
Authors: Jirapongsananuruk O, Malech HL, Kuhns DB, Niemela JE, Brown MR, Anderson-Cohen M, Fleisher TA.
Journal: J Allergy Clin Immunol (2003): 374
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123
Authors: Ischiropoulos H, Gow A, Thom SR, Kooy NW, Royall JA, Crow JP.
Journal: Methods Enzymol (1999): 367
Authors: Ischiropoulos H, Gow A, Thom SR, Kooy NW, Royall JA, Crow JP.
Journal: Methods Enzymol (1999): 367
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123
Authors: Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
Authors: Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species
Authors: Crow JP., undefined
Journal: Nitric Oxide (1997): 145
Authors: Crow JP., undefined
Journal: Nitric Oxide (1997): 145
Limitations on the use of dihydrorhodamine 123 for flow cytometric analysis of the neutrophil respiratory burst
Authors: van Pelt LJ, van Zwieten R, Weening RS, Roos D, Verhoeven AJ, Bolscher BG.
Journal: J Immunol Methods (1996): 187
Authors: van Pelt LJ, van Zwieten R, Weening RS, Roos D, Verhoeven AJ, Bolscher BG.
Journal: J Immunol Methods (1996): 187
Dihydrorhodamine 123 identifies impaired mitochondrial respiratory chain function in cultured cells harboring mitochondrial DNA mutations
Authors: Sobreira C, Davidson M, King MP, Mir and a AF., undefined
Journal: J Histochem Cytochem (1996): 571
Authors: Sobreira C, Davidson M, King MP, Mir and a AF., undefined
Journal: J Histochem Cytochem (1996): 571
Comparative use of 2,7-dichlorofluorescein diacetate, dihydrorhodamine 123, and hydroethidine for studying oxidative metabolism of phagocytosing cells
Authors: Biziukin AV, Korkina LG, Velichkovskii BT.
Journal: Biull Eksp Biol Med (1995): 361
Authors: Biziukin AV, Korkina LG, Velichkovskii BT.
Journal: Biull Eksp Biol Med (1995): 361
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