Dihydrorhodamine 123 *CAS 109244-58-8*

Dihydrorhodamine 123

CAS 109244-58-8

Dihydrorhodamine 123 is by far the most-used probe for measurement of intracellular H2O2. DHR 123 is oxidized directly to rhodamine 123, which is excitable at 488 and emits at 515 nm in the same emission range as FITC. It is widely used in human neutrophils, human eosinophils, HL60 cells, rat mast cells, guinea pig neutrophils, cultured chondrocytes, rat brain, rat renal proximal tubular cells, mesangial cells and L929 cells. In combination with other fluorescent reagents (such as surface receptor analysis by fluorescent antibodies, cell viability using propidium iodide, and calcium indicators) this probe can be used for multiplex measurements.

Protocol

COO

COA

Catalog	Size	Price	Quantity
15206	10 mg	Price
15207	5X1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Dihydrorhodamine 123 *CAS 109244-58-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	288.7 µL	1.444 mL	2.887 mL	14.435 mL	28.87 mL
5 mM	57.74 µL	288.7 µL	577.401 µL	2.887 mL	5.774 mL
10 mM	28.87 µL	144.35 µL	288.7 µL	1.444 mL	2.887 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	346.38 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Variations in reactive oxygen species generation by urban airborne particulate matter in lung epithelial cells—impact of inorganic fraction

Authors:

Mazuryk, Olga and Stochel, Grazyna and Brindell, Ma{\l}gorzata

Journal:

Frontiers in chemistry (2020): 581752

Direct macromolecular drug delivery to cerebral ischemia area using neutrophil-mediated nanoparticles

Authors:

Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin

Journal:

Theranostics (2017): 3260

Liver necrosis and lethal systemic inflammation in a murine model of Rickettsia typhi infection: role of neutrophils, macrophages and NK cells

Authors:

Papp, Stefanie and Moderzynski, Kristin and Rauch, Jessica and Heine, Liza and Kuehl, Svenja and Richardt, Ulricke and Mueller, Heidelinde and Fleischer, Bernhard and Osterloh, Anke

Journal:

PLoS Negl Trop Dis (2016): e0004935

Charakterisierung angeborener Immunmechanismen und immunpathologischer Effekte in der Rickettsia typhi-Infektion (Wolbach und Todd, 1920) in der Maus (Mus musculus, Linnaeus, 1758)

Authors:

Papp, Stefanie

Charakterisierung der immunmodulatorischen Funktion von Siglec-E (Sialins{\"a}ure-bindendes-Ig-{\"a}hnliches-Lektin-E) im Modell der experimentellen Chagas-Krankheit

Authors:

Heins, Anja

References

View all 20 references: Citation Explorer

Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD

Authors:

Mauch L, Lun A, O'Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler J.

Journal:

Clin Chem (2007): 890

Reactivity of 2',7'-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals

Authors:

Wrona M, Patel K, Wardman P.

Journal:

Free Radic Biol Med (2005): 262

Fluorescence measurements of steady state peroxynitrite production upon SIN-1 decomposition: NADH versus dihydrodichlorofluorescein and dihydrorhodamine 123

Authors:

Martin-Romero FJ, Gutierrez-Martin Y, Henao F, Gutierrez-Merino C.

Journal:

J Fluoresc (2004): 17

Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay

Authors:

Jirapongsananuruk O, Malech HL, Kuhns DB, Niemela JE, Brown MR, Anderson-Cohen M, Fleisher TA.

Journal:

J Allergy Clin Immunol (2003): 374

Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123

Authors:

Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.

Journal:

Free Radic Biol Med (1999): 146

Physical properties

Molecular weight	346.38
Solvent	DMSO

Spectral properties

Absorbance (nm)	497
Extinction coefficient (cm ^-1 M ^-1)	85200
Excitation (nm)	508
Emission (nm)	528

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	109244-58-8

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