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Dihydrorhodamine 123 *CAS 109244-58-8*

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Physical properties
Molecular weight346.38
Spectral properties
Absorbance (nm)497
Extinction coefficient (cm -1 M -1)85200
Excitation (nm)508
Emission (nm)528
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Show More (46)


Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Dihydrorhodamine 123 is by far the most-used probe for measurement of intracellular H2O2. DHR 123 is oxidized directly to rhodamine 123, which is excitable at 488 and emits at 515 nm in the same emission range as FITC. It is widely used in human neutrophils, human eosinophils, HL60 cells, rat mast cells, guinea pig neutrophils, cultured chondrocytes, rat brain, rat renal proximal tubular cells, mesangial cells and L929 cells. In combination with other fluorescent reagents (such as surface receptor analysis by fluorescent antibodies, cell viability using propidium iodide, and calcium indicators) this probe can be used for multiplex measurements.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Dihydrorhodamine 123 *CAS 109244-58-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM288.7 µL1.444 mL2.887 mL14.435 mL28.87 mL
5 mM57.74 µL288.7 µL577.401 µL2.887 mL5.774 mL
10 mM28.87 µL144.35 µL288.7 µL1.444 mL2.887 mL

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Spectral properties

Absorbance (nm)497
Extinction coefficient (cm -1 M -1)85200
Excitation (nm)508
Emission (nm)528

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Rhodamine 123 *CAS 62669-70-9*50852885200



View all 7 citations: Citation Explorer
Variations in reactive oxygen species generation by urban airborne particulate matter in lung epithelial cells—impact of inorganic fraction
Authors: Mazuryk, Olga and Stochel, Grazyna and Brindell, Ma{\l}gorzata
Journal: Frontiers in chemistry (2020): 581752
Direct macromolecular drug delivery to cerebral ischemia area using neutrophil-mediated nanoparticles
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin
Journal: Theranostics (2017): 3260
Liver necrosis and lethal systemic inflammation in a murine model of Rickettsia typhi infection: role of neutrophils, macrophages and NK cells
Authors: Papp, Stefanie and Moderzynski, Kristin and Rauch, Jessica and Heine, Liza and Kuehl, Svenja and Richardt, Ulricke and Mueller, Heidelinde and Fleischer, Bernhard and Osterloh, Anke
Journal: PLoS Negl Trop Dis (2016): e0004935
Bicarbonate plays a critical role in the generation of cytotoxicity during SIN-1 decomposition in culture medium
Authors: Shirai, Kyo and Okada, Tatsumi and Konishi, Kanako and Murata, Hiroshi and Akashi, Soichiro and Sugawara, Fumio and Watanabe, Nobuo and Arai, Takao
Journal: Oxidative medicine and cellular longevity (2012)
Direct Macromolecular Drug Delivery to Cerebral Ischemia Area using Neutrophil-Mediated Nanoparticles
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin


View all 20 references: Citation Explorer
Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD
Authors: Mauch L, Lun A, O'Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler J.
Journal: Clin Chem (2007): 890
Reactivity of 2',7'-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals
Authors: Wrona M, Patel K, Wardman P.
Journal: Free Radic Biol Med (2005): 262
Fluorescence measurements of steady state peroxynitrite production upon SIN-1 decomposition: NADH versus dihydrodichlorofluorescein and dihydrorhodamine 123
Authors: Martin-Romero FJ, Gutierrez-Martin Y, Henao F, Gutierrez-Merino C.
Journal: J Fluoresc (2004): 17
Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay
Authors: Jirapongsananuruk O, Malech HL, Kuhns DB, Niemela JE, Brown MR, Anderson-Cohen M, Fleisher TA.
Journal: J Allergy Clin Immunol (2003): 374
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123
Authors: Ischiropoulos H, Gow A, Thom SR, Kooy NW, Royall JA, Crow JP.
Journal: Methods Enzymol (1999): 367
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123
Authors: Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species
Authors: Crow JP., undefined
Journal: Nitric Oxide (1997): 145
Limitations on the use of dihydrorhodamine 123 for flow cytometric analysis of the neutrophil respiratory burst
Authors: van Pelt LJ, van Zwieten R, Weening RS, Roos D, Verhoeven AJ, Bolscher BG.
Journal: J Immunol Methods (1996): 187
Dihydrorhodamine 123 identifies impaired mitochondrial respiratory chain function in cultured cells harboring mitochondrial DNA mutations
Authors: Sobreira C, Davidson M, King MP, Mir and a AF., undefined
Journal: J Histochem Cytochem (1996): 571
Comparative use of 2,7-dichlorofluorescein diacetate, dihydrorhodamine 123, and hydroethidine for studying oxidative metabolism of phagocytosing cells
Authors: Biziukin AV, Korkina LG, Velichkovskii BT.
Journal: Biull Eksp Biol Med (1995): 361