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Rhodamine 110 *CAS 13558-31-1*
Rhodamine 110 (R110) is the precursor for a variety of R110-based enzyme substrates. It is also used as a calibration standard for R110-based enzyme substrates. Compared to AMC and AFC enzyme substrates, fluorogenic R110 substrates are generally more sensitive, giving longer absorption and fluorescence wavelength than AMC and AFC substrates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 110 *CAS 13558-31-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 272.628 µL | 1.363 mL | 2.726 mL | 13.631 mL | 27.263 mL |
| 5 mM | 54.526 µL | 272.628 µL | 545.256 µL | 2.726 mL | 5.453 mL |
| 10 mM | 27.263 µL | 136.314 µL | 272.628 µL | 1.363 mL | 2.726 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 | - |
| Rhodamine 6G *CAS 989-38-8* | 525 | 548 | 1160001 | 0.951 |
| Rhodamine B *CAS 81-88-9* | 546 | 567 | 1060001 | 0.71, 0.432 |
Citations
View all 2 citations: Citation Explorer
Exploring glutathione-decorated micelles for drug delivery: a promise for enhanced cellular uptake
Authors: Truszkowska, Martyna and Reisenberger, Elsa and Kali, Gergely and Stengel, Daniel and Saleh, Ahmad and Seybold, Anna and Kipura, Tobias and Kwiatkowski, Marcel and Bernkop-Schn{\"u}rch, Andreas
Journal: Colloids and Surfaces B: Biointerfaces (2025): 114664
Authors: Truszkowska, Martyna and Reisenberger, Elsa and Kali, Gergely and Stengel, Daniel and Saleh, Ahmad and Seybold, Anna and Kipura, Tobias and Kwiatkowski, Marcel and Bernkop-Schn{\"u}rch, Andreas
Journal: Colloids and Surfaces B: Biointerfaces (2025): 114664
Phosphatase-degradable Nanoparticles: A game-changing approach for the delivery of antifungal proteins
Authors: Akku{\c{s}}-Da{\u{g}}deviren, Zeynep Burcu and Saleh, Ahmad and Sch{\"o}pf, Cristina and Truszkowska, Martyna and Bratschun-Khan, Doris and F{\"u}rst, Andrea and Seybold, Anna and Offterdinger, Martin and Marx, Florentine and Bernkop-Schn{\"u}rch, Andreas
Journal: Journal of Colloid and Interface Science (2023)
Authors: Akku{\c{s}}-Da{\u{g}}deviren, Zeynep Burcu and Saleh, Ahmad and Sch{\"o}pf, Cristina and Truszkowska, Martyna and Bratschun-Khan, Doris and F{\"u}rst, Andrea and Seybold, Anna and Offterdinger, Martin and Marx, Florentine and Bernkop-Schn{\"u}rch, Andreas
Journal: Journal of Colloid and Interface Science (2023)
References
View all 8 references: Citation Explorer
N-DEVD-N'-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay
Authors: Wang ZQ, Liao J, Diwu Z.
Journal: Bioorg Med Chem Lett (2005): 2335
Authors: Wang ZQ, Liao J, Diwu Z.
Journal: Bioorg Med Chem Lett (2005): 2335
Fluorogenic substrate [Ala-Pro]2-cresyl violet but not Ala-Pro-rhodamine 110 is cleaved specifically by DPPIV activity: a study in living Jurkat cells and CD26/DPPIV-transfected Jurkat cells
Authors: Boonacker E, Elferink S, Bardai A, Fleischer B, Van Noorden CJ.
Journal: J Histochem Cytochem (2003): 959
Authors: Boonacker E, Elferink S, Bardai A, Fleischer B, Van Noorden CJ.
Journal: J Histochem Cytochem (2003): 959
A transgenic mouse model with a luciferase reporter for studying in vivo transcriptional regulation of the human CYP3A4 gene
Authors: Zhang W, Purchio AF, Chen K, Wu J, Lu L, Coffee R, Contag PR, West DB.
Journal: Drug Metab Dispos (2003): 1054
Authors: Zhang W, Purchio AF, Chen K, Wu J, Lu L, Coffee R, Contag PR, West DB.
Journal: Drug Metab Dispos (2003): 1054
Design and synthesis of rhodamine 110 derivative and caspase-3 substrate for enzyme and cell-based fluorescent assay
Authors: Cai SX, Zhang HZ, Guastella J, Drewe J, Yang W, Weber E.
Journal: Bioorg Med Chem Lett (2001): 39
Authors: Cai SX, Zhang HZ, Guastella J, Drewe J, Yang W, Weber E.
Journal: Bioorg Med Chem Lett (2001): 39
Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells
Authors: Hug H, Los M, Hirt W, Debatin KM.
Journal: Biochemistry (1999): 13906
Authors: Hug H, Los M, Hirt W, Debatin KM.
Journal: Biochemistry (1999): 13906
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