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Rhodamine 110 *CAS 13558-31-1*

Chemical structure for Rhodamine 110 *CAS 13558-31-1*
Chemical structure for Rhodamine 110 *CAS 13558-31-1*
Ordering information
Price ()
Catalog Number86
Unit Size
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Additional ordering information
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Fax1-408-733-1304
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight366.8
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)80000
Excitation (nm)500
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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5-CR110 [5-Carboxyrhodamine 110] *Single isomer*
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Show More (51)

OverviewpdfSDSpdfProtocol


CAS
13558-31-1
Molecular weight
366.8
Extinction coefficient (cm -1 M -1)
80000
Excitation (nm)
500
Emission (nm)
522
Rhodamine 110 (R110) is the precursor for a variety of R110-based enzyme substrates. It is also used as a calibration standard for R110-based enzyme substrates. Compared to AMC and AFC enzyme substrates, fluorogenic R110 substrates are generally more sensitive, giving longer absorption and fluorescence wavelength than AMC and AFC substrates.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 110 *CAS 13558-31-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM272.628 µL1.363 mL2.726 mL13.631 mL27.263 mL
5 mM54.526 µL272.628 µL545.256 µL2.726 mL5.453 mL
10 mM27.263 µL136.314 µL272.628 µL1.363 mL2.726 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)80000
Excitation (nm)500
Emission (nm)522

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
Rhodamine 700 *CAS 63561-42-2*---
Rhodamine 800 *CAS 137993-41-0*682704-
Rhodamine B *CAS 81-88-9*5465681060001
Rhodamine 6G *CAS 989-38-8*5255481160001
Rhodamine 123 *CAS 62669-70-9*50852885200
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000

References


View all 8 references: Citation Explorer
N-DEVD-N'-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay
Authors: Wang ZQ, Liao J, Diwu Z.
Journal: Bioorg Med Chem Lett (2005): 2335
A transgenic mouse model with a luciferase reporter for studying in vivo transcriptional regulation of the human CYP3A4 gene
Authors: Zhang W, Purchio AF, Chen K, Wu J, Lu L, Coffee R, Contag PR, West DB.
Journal: Drug Metab Dispos (2003): 1054
Fluorogenic substrate [Ala-Pro]2-cresyl violet but not Ala-Pro-rhodamine 110 is cleaved specifically by DPPIV activity: a study in living Jurkat cells and CD26/DPPIV-transfected Jurkat cells
Authors: Boonacker E, Elferink S, Bardai A, Fleischer B, Van Noorden CJ.
Journal: J Histochem Cytochem (2003): 959
Design and synthesis of rhodamine 110 derivative and caspase-3 substrate for enzyme and cell-based fluorescent assay
Authors: Cai SX, Zhang HZ, Guastella J, Drewe J, Yang W, Weber E.
Journal: Bioorg Med Chem Lett (2001): 39
Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells
Authors: Hug H, Los M, Hirt W, Debatin KM.
Journal: Biochemistry (1999): 13906
Intracellular accumulation of rhodamine 110 in single living cells
Authors: Jeannot V, Salmon JM, Deumie M, Viallet P.
Journal: J Histochem Cytochem (1997): 403
Flow cytometric determination of aminopeptidase activities in viable cells using fluorogenic rhodamine 110 substrates
Authors: Ganesh S, Klingel S, Kahle H, Valet G.
Journal: Cytometry (1995): 334
Flow cytometric determination of cysteine and serine proteinase activities in living cells with rhodamine 110 substrates
Authors: Klingel S, Rothe G, Kellermann W, Valet G.
Journal: Methods Cell Biol (1994): 449