Rhodamine 110 *CAS 13558-31-1*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
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Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 366.8 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 80000 |
Excitation (nm) | 500 |
Emission (nm) | 522 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
CAS 13558-31-1 | Molecular weight 366.8 | Extinction coefficient (cm -1 M -1) 80000 | Excitation (nm) 500 | Emission (nm) 522 |
Rhodamine 110 (R110) is the precursor for a variety of R110-based enzyme substrates. It is also used as a calibration standard for R110-based enzyme substrates. Compared to AMC and AFC enzyme substrates, fluorogenic R110 substrates are generally more sensitive, giving longer absorption and fluorescence wavelength than AMC and AFC substrates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 110 *CAS 13558-31-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 272.628 µL | 1.363 mL | 2.726 mL | 13.631 mL | 27.263 mL |
5 mM | 54.526 µL | 272.628 µL | 545.256 µL | 2.726 mL | 5.453 mL |
10 mM | 27.263 µL | 136.314 µL | 272.628 µL | 1.363 mL | 2.726 mL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 80000 |
Excitation (nm) | 500 |
Emission (nm) | 522 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Rhodamine 800 *CAS 137993-41-0* | 682 | 704 | - |
Rhodamine B *CAS 81-88-9* | 546 | 567 | 1060001 |
Rhodamine 6G *CAS 989-38-8* | 525 | 548 | 1160001 |
Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 |
Rhodamine aldehyde [5-TAMRA aldehyde] | 552 | 578 | 90000 |
Citations
View all 1 citations: Citation Explorer
Phosphatase-degradable Nanoparticles: A game-changing approach for the delivery of antifungal proteins
Authors: Akku{\c{s}}-Da{\u{g}}deviren, Zeynep Burcu and Saleh, Ahmad and Sch{\"o}pf, Cristina and Truszkowska, Martyna and Bratschun-Khan, Doris and F{\"u}rst, Andrea and Seybold, Anna and Offterdinger, Martin and Marx, Florentine and Bernkop-Schn{\"u}rch, Andreas
Journal: Journal of Colloid and Interface Science (2023)
Authors: Akku{\c{s}}-Da{\u{g}}deviren, Zeynep Burcu and Saleh, Ahmad and Sch{\"o}pf, Cristina and Truszkowska, Martyna and Bratschun-Khan, Doris and F{\"u}rst, Andrea and Seybold, Anna and Offterdinger, Martin and Marx, Florentine and Bernkop-Schn{\"u}rch, Andreas
Journal: Journal of Colloid and Interface Science (2023)
References
View all 8 references: Citation Explorer
N-DEVD-N'-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay
Authors: Wang ZQ, Liao J, Diwu Z.
Journal: Bioorg Med Chem Lett (2005): 2335
Authors: Wang ZQ, Liao J, Diwu Z.
Journal: Bioorg Med Chem Lett (2005): 2335
A transgenic mouse model with a luciferase reporter for studying in vivo transcriptional regulation of the human CYP3A4 gene
Authors: Zhang W, Purchio AF, Chen K, Wu J, Lu L, Coffee R, Contag PR, West DB.
Journal: Drug Metab Dispos (2003): 1054
Authors: Zhang W, Purchio AF, Chen K, Wu J, Lu L, Coffee R, Contag PR, West DB.
Journal: Drug Metab Dispos (2003): 1054
Fluorogenic substrate [Ala-Pro]2-cresyl violet but not Ala-Pro-rhodamine 110 is cleaved specifically by DPPIV activity: a study in living Jurkat cells and CD26/DPPIV-transfected Jurkat cells
Authors: Boonacker E, Elferink S, Bardai A, Fleischer B, Van Noorden CJ.
Journal: J Histochem Cytochem (2003): 959
Authors: Boonacker E, Elferink S, Bardai A, Fleischer B, Van Noorden CJ.
Journal: J Histochem Cytochem (2003): 959
Design and synthesis of rhodamine 110 derivative and caspase-3 substrate for enzyme and cell-based fluorescent assay
Authors: Cai SX, Zhang HZ, Guastella J, Drewe J, Yang W, Weber E.
Journal: Bioorg Med Chem Lett (2001): 39
Authors: Cai SX, Zhang HZ, Guastella J, Drewe J, Yang W, Weber E.
Journal: Bioorg Med Chem Lett (2001): 39
Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells
Authors: Hug H, Los M, Hirt W, Debatin KM.
Journal: Biochemistry (1999): 13906
Authors: Hug H, Los M, Hirt W, Debatin KM.
Journal: Biochemistry (1999): 13906
Intracellular accumulation of rhodamine 110 in single living cells
Authors: Jeannot V, Salmon JM, Deumie M, Viallet P.
Journal: J Histochem Cytochem (1997): 403
Authors: Jeannot V, Salmon JM, Deumie M, Viallet P.
Journal: J Histochem Cytochem (1997): 403
Flow cytometric determination of aminopeptidase activities in viable cells using fluorogenic rhodamine 110 substrates
Authors: Ganesh S, Klingel S, Kahle H, Valet G.
Journal: Cytometry (1995): 334
Authors: Ganesh S, Klingel S, Kahle H, Valet G.
Journal: Cytometry (1995): 334
Flow cytometric determination of cysteine and serine proteinase activities in living cells with rhodamine 110 substrates
Authors: Klingel S, Rothe G, Kellermann W, Valet G.
Journal: Methods Cell Biol (1994): 449
Authors: Klingel S, Rothe G, Kellermann W, Valet G.
Journal: Methods Cell Biol (1994): 449
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
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A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets