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Rhodamine 6G *CAS 989-38-8*

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Physical properties
Molecular weight479.01
Spectral properties
Absorbance (nm)530
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)525
Emission (nm)548
Quantum yield0.951
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Rhodamine 6G is also called Rhodamine 590, R6G, Basic Rhodamine Yellow , or C.I. 45160. Rhodamine 6G is often used in a laser dye pumped by the 2nd (532 nm) harmonic from a Nd:YAG laser since it has remarkably high photostability, high quantum yield, low cost, and close proximity to the absorption maximum (approximately 530 nm). The lasering range is 555 to 585 nm with a maximum at 566 nm. Rhodamine 6G is also useful in Pgp efflux assays, and has been used to characterize kinetics of MRP1-mediated efflux.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 6G *CAS 989-38-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM208.764 µL1.044 mL2.088 mL10.438 mL20.876 mL
5 mM41.753 µL208.764 µL417.528 µL2.088 mL4.175 mL
10 mM20.876 µL104.382 µL208.764 µL1.044 mL2.088 mL

Molarity calculator

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Spectral properties

Absorbance (nm)530
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)525
Emission (nm)548
Quantum yield0.951

Product Family

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Rhodamine 800 *CAS 137993-41-0*682704-
Rhodamine B *CAS 81-88-9*5465671060001
Rhodamine 110 *CAS 13558-31-1*50052280000
Rhodamine 123 *CAS 62669-70-9*50852885200
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000



View all 33 references: Citation Explorer
Resonance Raman scattering of rhodamine 6G as calculated using time-dependent density functional theory
Authors: Jensen L, Schatz GC.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5973
Exploitation of Trichoderma harzianum mycelial waste for the removal of rhodamine 6G from aqueous solution
Authors: Sadhasivam S, Savitha S, Swaminathan K.
Journal: J Environ Manage. (2006)
Determination of alkaline phosphatase based on affinity adsorption solid-substrate room temperature phosphorimetry using rhodamine 6G-dibromoluciferin luminescent nanoparticle to label lectin and prediction of diseases
Authors: Liu JM, Liu ZB, Hu LX, He HX, Yang ML, Zhou P, Chen XH, Zheng MM, Zeng XY, Xu YL.
Journal: Anal Biochem (2006): 173
Molecular-dynamics simulations of pyronine 6G and rhodamine 6G dimers in aqueous solution
Authors: Chuichay P, Vladimirov E, Siriwong K, Hannongbua S, Rosch N.
Journal: J Mol Model (Online) (2006): 885
Preparation of highly monodisperse poly(methyl methacrylate) particles incorporating fluorescent rhodamine 6G for colloidal crystals
Authors: Nagao D, Anzai N, Kobayashi Y, Gu S, Konno M.
Journal: J Colloid Interface Sci (2006): 232
Fluorescent plasma nanocomposite thin films containing nonaggregated rhodamine 6G laser dye molecules
Authors: Barranco A, Groening P.
Journal: Langmuir (2006): 6719
Ab initio study of optical properties of rhodamine 6G molecular dimers
Authors: Gavrilenko VI, Noginov MA.
Journal: J Chem Phys (2006): 44301
Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Spectroscopic studies of rhodamine 6G dispersed in polymethylcyanoacrylate
Authors: Saini GS, Kaur S, Tripathi SK, Mahajan CG, Thanga HH, Verma AL.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2005): 653
Study on sodium lauryl sulfate (SDS) induced fluorescence enhancement of rhodamine 6G in water solution excited by 532 nm laser
Authors: He YH, Cheng J, Zuo HY, Yang JG.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2005): 648