Rhodamine 6G CAS 989-38-8

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Physical properties

Molecular weight	479.01
Solvent	DMSO

Spectral properties

Absorbance (nm)	530
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	525
Emission (nm)	548
Quantum yield	0.95¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

989-38-8

Molecular weight

479.01

Absorbance (nm)

530

Extinction coefficient (cm ^-1 M ^-1)

116000¹

Excitation (nm)

525

Emission (nm)

548

Quantum yield

0.95¹

Rhodamine 6G is also called Rhodamine 590, R6G, Basic Rhodamine Yellow , or C.I. 45160. Rhodamine 6G is often used in a laser dye pumped by the 2nd (532 nm) harmonic from a Nd:YAG laser since it has remarkably high photostability, high quantum yield, low cost, and close proximity to the absorption maximum (approximately 530 nm). The lasering range is 555 to 585 nm with a maximum at 566 nm. Rhodamine 6G is also useful in Pgp efflux assays, and has been used to characterize kinetics of MRP1-mediated efflux.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine 6G *CAS 989-38-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	208.764 µL	1.044 mL	2.088 mL	10.438 mL	20.876 mL
5 mM	41.753 µL	208.764 µL	417.528 µL	2.088 mL	4.175 mL
10 mM	20.876 µL	104.382 µL	208.764 µL	1.044 mL	2.088 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	530
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	525
Emission (nm)	548
Quantum yield	0.95¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
Rhodamine 800 CAS 137993-41-0	682	704	-
Rhodamine B CAS 81-88-9	546	567	106000¹
Rhodamine 110 CAS 13558-31-1	500	522	80000
Rhodamine 123 CAS 62669-70-9	508	528	85200
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000

Images

Figure 1. Chemical structure for Rhodamine 6G *CAS 989-38-8*

References

View all 33 references: Citation Explorer

Resonance Raman scattering of rhodamine 6G as calculated using time-dependent density functional theory
Authors: Jensen L, Schatz GC.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2006): 5973

Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)

Ab initio study of optical properties of rhodamine 6G molecular dimers
Authors: Gavrilenko VI, Noginov MA.
Journal: J Chem Phys (2006): 44301

Fluorescent plasma nanocomposite thin films containing nonaggregated rhodamine 6G laser dye molecules
Authors: Barranco A, Groening P.
Journal: Langmuir (2006): 6719

Preparation of highly monodisperse poly(methyl methacrylate) particles incorporating fluorescent rhodamine 6G for colloidal crystals
Authors: Nagao D, Anzai N, Kobayashi Y, Gu S, Konno M.
Journal: J Colloid Interface Sci (2006): 232

Molecular-dynamics simulations of pyronine 6G and rhodamine 6G dimers in aqueous solution
Authors: Chuichay P, Vladimirov E, Siriwong K, Hannongbua S, Rosch N.
Journal: J Mol Model (Online) (2006): 885

Determination of alkaline phosphatase based on affinity adsorption solid-substrate room temperature phosphorimetry using rhodamine 6G-dibromoluciferin luminescent nanoparticle to label lectin and prediction of diseases
Authors: Liu JM, Liu ZB, Hu LX, He HX, Yang ML, Zhou P, Chen XH, Zheng MM, Zeng XY, Xu YL.
Journal: Anal Biochem (2006): 173

Exploitation of Trichoderma harzianum mycelial waste for the removal of rhodamine 6G from aqueous solution
Authors: Sadhasivam S, Savitha S, Swaminathan K.
Journal: J Environ Manage. (2006)

Study on sodium lauryl sulfate (SDS) induced fluorescence enhancement of rhodamine 6G in water solution excited by 532 nm laser
Authors: He YH, Cheng J, Zuo HY, Yang JG.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2005): 648

Study of energy transfer from 7-amino coumarin donors to the rhodamine 6G acceptor in lecithin vesicles and sodium taurocholate-lecithin mixed aggregates
Authors: Seth D, Chakraborty A, Setua P, Chakrabarty D, Sarkar N.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2005): 12080