6XHis alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1120.21 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
6XHis Succinimidyl Ester |
6XHis maleimide |
6XHis azide |
Overview | SDSProtocol |
See also: Affinity Purification, Click Chemistry
Molecular weight 1120.21 |
6XHis alkyne is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry. It readily reacts with a biomolecule that contains an azido group. The 6xHis tag is the most common his-tag and has a molecular weight of ~0.8kDa. His-tags (i.e., polyhistidine tags) comprise a consecutive series of six to ten histidine residues. His tags are used for many recombinant proteins to facilitate purification, allowing researchers to extract a protein of interest from thousands of other proteins found in a cell or cell lysate. The small size of the 6x-His tag has a lower possibility for the tag to affect the functionality of the fusion protein. Histidine forms coordination bonds with immobilized transition metal ions, and this property can be utilized for protein purification. His-tag protein purification is by a specialized form of affinity chromatography, called immobilized metal affinity chromatography (IMAC), where proteins or peptides are separated according to their affinity for metal ions immobilized to a solid chelating resin. During this process, a small His-tag is fused to either the N or C terminus of the target protein, enabling capture by nickel or cobalt ions coordinated on a variety of resins. The small size, low cost, and ease of use have made the His-tag the most popular affinity-tag available. AAT Bioquest offers the largest collection of 6XHis, NTA, bis-NTA, tris-NTA and IDA reagents for His Tag detections and purifications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6XHis alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 89.269 µL | 446.345 µL | 892.69 µL | 4.463 mL | 8.927 mL |
5 mM | 17.854 µL | 89.269 µL | 178.538 µL | 892.69 µL | 1.785 mL |
10 mM | 8.927 µL | 44.634 µL | 89.269 µL | 446.345 µL | 892.69 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
AMCA Alkyne | 346 | 434 | 19000 | 0.153 |
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
ICG alkyne | 789 | 813 | 230000 | 0.076 |
Cy3B alkyne | 560 | 571 | 1200001 | 0.069 |
XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
Images
References
View all 50 references: Citation Explorer
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Authors: Souza, Marilen and Machado, Juliana and da Silva, Jonatas and Ramos, Luana and Nogueira, Lais and Ribeiro, Patrícia and Dias, Daniel and Santos, Josiane and Santos, José Carlos and Nóbrega, Yanna and Souza, Amanda and Freitas, Sonia and da Paz, Mariana Campos and Felipe, Maria and Torres, Fernando and Galdino, Alexsandro
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Localized cardiolipin synthesis is required for the assembly of MreB during the polarized cell division of Chlamydia trachomatis.
Authors: Ouellette, Scot P and Fisher-Marvin, Laura A and Harpring, McKenna and Lee, Junghoon and Rucks, Elizabeth A and Cox, John V
Journal: PLoS pathogens (2022): e1010836
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The first step into phenolic metabolism in the hornwort Anthoceros agrestis: molecular and biochemical characterization of two phenylalanine ammonia-lyase isoforms.
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