6XHis maleimide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1161.22 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
6XHis Succinimidyl Ester |
6XHis azide |
6XHis alkyne |
Overview | SDSProtocol |
See also: Affinity Purification
Molecular weight 1161.22 |
6XHis maleimide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools. It readily reacts with a biomolecule that contains a thiol group such as antibodies. The 6xHis tag is the most common his-tag and has a molecular weight of ~0.8kDa. His-tags (i.e., polyhistidine tags) comprise a consecutive series of six to ten histidine residues. His tags are used for many recombinant proteins to facilitate purification, allowing researchers to extract a protein of interest from thousands of other proteins found in a cell or cell lysate. The small size of the 6x-His tag has a lower possibility for the tag to affect the functionality of the fusion protein. Histidine forms coordination bonds with immobilized transition metal ions, and this property can be utilized for protein purification. His-tag protein purification is by a specialized form of affinity chromatography, called immobilized metal affinity chromatography (IMAC), where proteins or peptides are separated according to their affinity for metal ions immobilized to a solid chelating resin. During this process, a small His-tag is fused to either the N or C terminus of the target protein, enabling capture by nickel or cobalt ions coordinated on a variety of resins. The small size, low cost, and ease of use have made the His-tag the most popular affinity-tag available. AAT Bioquest offers the largest collection of 6XHis, NTA, bis-NTA, tris-NTA and IDA reagents for His Tag detections and purifications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6XHis maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 86.116 µL | 430.582 µL | 861.163 µL | 4.306 mL | 8.612 mL |
5 mM | 17.223 µL | 86.116 µL | 172.233 µL | 861.163 µL | 1.722 mL |
10 mM | 8.612 µL | 43.058 µL | 86.116 µL | 430.582 µL | 861.163 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 6 references: Citation Explorer
Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose.
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Expression, purification, and functional characterisation of flagellin, a TLR5-ligand.
Authors: Hajam, Irshad Ahmed and Dar, Pervaiz Ahmed and Sekar, Shanmugam Chandra and Nanda, Rajakishore and Kishore, Subodh and Bhanuprakash, Veerakyathappa and Ganesh, Kondabattula
Journal: Veterinaria italiana (2013): 181-6
Authors: Hajam, Irshad Ahmed and Dar, Pervaiz Ahmed and Sekar, Shanmugam Chandra and Nanda, Rajakishore and Kishore, Subodh and Bhanuprakash, Veerakyathappa and Ganesh, Kondabattula
Journal: Veterinaria italiana (2013): 181-6
Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase.
Authors: Maliekal, Pushpa and Vertommen, Didier and Delpierre, Ghislain and Van Schaftingen, Emile
Journal: Glycobiology (2006): 165-72
Authors: Maliekal, Pushpa and Vertommen, Didier and Delpierre, Ghislain and Van Schaftingen, Emile
Journal: Glycobiology (2006): 165-72
Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii.
Authors: Grassick, Alice and Murray, Patrick G and Thompson, Roisin and Collins, Catherine M and Byrnes, Lucy and Birrane, Gabriel and Higgins, Timothy M and Tuohy, Maria G
Journal: European journal of biochemistry (2004): 4495-506
Authors: Grassick, Alice and Murray, Patrick G and Thompson, Roisin and Collins, Catherine M and Byrnes, Lucy and Birrane, Gabriel and Higgins, Timothy M and Tuohy, Maria G
Journal: European journal of biochemistry (2004): 4495-506
Expression of functional melanocortin 1 receptors in insect cells.
Authors: Schiöth, H B and Kuusinen, A and Muceniece, R and Szardenings, M and Keinänen, K and Wikberg, J E
Journal: Biochemical and biophysical research communications (1996): 807-14
Authors: Schiöth, H B and Kuusinen, A and Muceniece, R and Szardenings, M and Keinänen, K and Wikberg, J E
Journal: Biochemical and biophysical research communications (1996): 807-14
Application notes
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