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7-AAD
7-Aminoactinomycin D; CAS 7240-37-1
7-AAD (7-aminoactinomycin D) is a cell-impermeant, G-C selective DNA intercalating dye with red fluorescence that specifically stains dead and necrotic cells for viability assessment in flow cytometry and microscopy.
  • G-C Selective DNA Intercalation: High affinity for GC-rich regions with red fluorescence (Ex/Em = 549/648 nm)
  • Cell Impermeant Viability Dye: Specifically stains dead and necrotic cells with compromised membranes
  • 488 nm Laser Compatible: Large Stokes shift reduces spectral overlap with FITC and PE in multicolor flow cytometry
  • Lower Quantum Yield vs PI: QY = 0.02 provides controlled fluorescence intensity for sensitive detection
7-AAD spectrum. 7-AAD (7-Aminoactinomycin D) is a fluorescent compound with an excitation peak at 549 nm and an emission peak at 648 nm. Other spectra of interest include: Propidium iodide, Acridine Orange, and DAPI (4,6-Diamidino-2-phenylindole). 7-AAD (7-Aminoactinomycin D) belongs to the following categories: Cell Cycle Assays, Nucleus, Cell Viability Assays, and Fluorescence Activated Cell Sorting (FACS).
7-AAD spectrum. 7-AAD (7-Aminoactinomycin D) is a fluorescent compound with an excitation peak at 549 nm and an emission peak at 648 nm. Other spectra of interest include: Propidium iodide, Acridine Orange, and DAPI (4,6-Diamidino-2-phenylindole). 7-AAD (7-Aminoactinomycin D) belongs to the following categories: Cell Cycle Assays, Nucleus, Cell Viability Assays, and Fluorescence Activated Cell Sorting (FACS).
CatalogSize
Price
Quantity
175011 mg
Price
 
Physical properties

Molecular weight1270.43
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
27500
1
Excitation (nm)549
Emission (nm)648
Quantum yield
0.02
1
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
CAS7240-37-1
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Page updated on September 24, 2024