7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1270.43 |
| Solvent | DMSO |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 275001 |
| Excitation (nm) | 549 |
| Emission (nm) | 648 |
| Quantum yield | 0.021 |
Storage, safety and handling
| H-phrase | H303, H313, H340 |
| Hazard symbol | T |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R68 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41116134 |
| Overview |  SDSProtocol |
See also: Cell Cycle Assays, Cell Structures and Organelles, Nucleus, Cell Viability Assays, Cellular Processes, Flow Cytometry Reagents, Fluorescence Activated Cell Sorting (FACS)
CAS 7240-37-1 | Molecular weight 1270.43 | Extinction coefficient (cm -1 M -1) 275001 | Excitation (nm) 549 | Emission (nm) 648 | Quantum yield 0.021 |
7-Amino actinomycin D (7-AAD) is a non-permeant dye that can be used to identify non-viable cells. Cells with damaged plasma membranes or with impaired/no cell metabolism are unable to prevent the dye from entering the cell. Once inside the cell, the dyes bind to intracellular DNA producing highly fluorescent adducts which identify the cells as "non-viable". It is widely used in flow cytometry. 7-AAD is excited by the 488 nm laser line of an argon laser with fluorescence detected above 650 nm. Although the emission intensity of 7-AAD is lower than that of PI, the longer wavelength emission may make it more useful for multiplexing assays in combination with other 488 nm-excited fluorochromes such as FITC and PE.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 78.714 µL | 393.568 µL | 787.135 µL | 3.936 mL | 7.871 mL |
| 5 mM | 15.743 µL | 78.714 µL | 157.427 µL | 787.135 µL | 1.574 mL |
| 10 mM | 7.871 µL | 39.357 µL | 78.714 µL | 393.568 µL | 787.135 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 275001 |
| Excitation (nm) | 549 |
| Emission (nm) | 648 |
| Quantum yield | 0.021 |
Images
Figure 1. Chemical structure for 7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1*
Citations
View all 13 citations: Citation Explorer
Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants
Authors: Aabling, Rasmus Roost and Alstrup, Toke and Kj{\ae}r, Emma Mader and Poulsen, Kristine Juul and Pedersen, Jonas Oute and Revenfeld, Anne Louise and M{\o}ller, Bjarne Kuno and Eijken, Marco
Journal: Regenerative Therapy (2023): 67--75
Authors: Aabling, Rasmus Roost and Alstrup, Toke and Kj{\ae}r, Emma Mader and Poulsen, Kristine Juul and Pedersen, Jonas Oute and Revenfeld, Anne Louise and M{\o}ller, Bjarne Kuno and Eijken, Marco
Journal: Regenerative Therapy (2023): 67--75
Development and improved fabrication of microfluidic devices for local delivery to investigate properties of healthy and inflamed lymph node tissue ex vivo slices.
Authors: Catterton, Megan Ann
Journal: (2021)
Authors: Catterton, Megan Ann
Journal: (2021)
Rapid fabrication by digital light processing 3D printing of a SlipChip with movable ports for local delivery to ex vivo organ cultures
Authors: Catterton, Megan and Ball, Alexander and Pompano, Rebecca
Journal: (2021)
Authors: Catterton, Megan and Ball, Alexander and Pompano, Rebecca
Journal: (2021)
USP9X deubiquitinase couples the pluripotency network and cell metabolism to regulate ESC differentiation potential
Authors: de Dieuleveult, Maud and Leduc, Marjorie and Salataj, Eralda and Ransy, C{\'e}line and Dairou, Julien and Homma, Kengo and Le Gall, Morgane and Bossard, Pascale and Lomb{\`e}s, Anne and Bouillaud, Fr{\'e}d{\'e}ric and others,
Journal: bioRxiv (2020)
Authors: de Dieuleveult, Maud and Leduc, Marjorie and Salataj, Eralda and Ransy, C{\'e}line and Dairou, Julien and Homma, Kengo and Le Gall, Morgane and Bossard, Pascale and Lomb{\`e}s, Anne and Bouillaud, Fr{\'e}d{\'e}ric and others,
Journal: bioRxiv (2020)
Labelling primary immune cells using bright blue fluorescent nanoparticles
Authors: Belanger, Maura C and Zhuang, Meng and Ball, Alexander G and Richey, Kristen H and DeRosa, Christopher A and Fraser, Cassandra L and Pompano, Rebecca R
Journal: Biomaterials science (2020): 1897--1909
Authors: Belanger, Maura C and Zhuang, Meng and Ball, Alexander G and Richey, Kristen H and DeRosa, Christopher A and Fraser, Cassandra L and Pompano, Rebecca R
Journal: Biomaterials science (2020): 1897--1909
The lncRNA Hand2os1/Uph locus orchestrates heart development through regulation of precise expression of Hand2
Authors: Han, Xue and Zhang, Jiejie and Liu, Yaxi and Fan, Xiaoying and Ai, Shanshan and Luo, Yingjie and Li, Xin and Jin, Hengwei and Luo, Sai and Zheng, Hui and others,
Journal: Development (2019): dev176198
Authors: Han, Xue and Zhang, Jiejie and Liu, Yaxi and Fan, Xiaoying and Ai, Shanshan and Luo, Yingjie and Li, Xin and Jin, Hengwei and Luo, Sai and Zheng, Hui and others,
Journal: Development (2019): dev176198
Synergistic Nanoparticle Formulations against Multi-drug Resistant Breast Cancers
Authors: Czupiel, Petro Pawlo
Journal: (2019)
Authors: Czupiel, Petro Pawlo
Journal: (2019)
PD-1 silencing impairs the anti-tumor function of chimeric antigen receptor modified T cells by inhibiting proliferation activity
Authors: Wei, Jianshu and Luo, Can and Wang, Yao and Guo, Yelei and Dai, Hanren and Tong, Chuan and Ti, Dongdong and Wu, Zhiqiang and Han, Weidong
Journal: Journal for immunotherapy of cancer (2019): 1--15
Authors: Wei, Jianshu and Luo, Can and Wang, Yao and Guo, Yelei and Dai, Hanren and Tong, Chuan and Ti, Dongdong and Wu, Zhiqiang and Han, Weidong
Journal: Journal for immunotherapy of cancer (2019): 1--15
The long noncoding RNA Hand2as orchestrates heart development through regulation of precise expression of HAND2
Authors: Han, Xue and Zhang, Jiejie and Liu, Yaxi and Fan, Xiaoying and Ai, Shanshan and Luo, Yingjie and Li, Xin and Luo, Sai and Zheng, Hui and Yue, Yanzhu and others,
Journal: bioRxiv (2018): 475723
Authors: Han, Xue and Zhang, Jiejie and Liu, Yaxi and Fan, Xiaoying and Ai, Shanshan and Luo, Yingjie and Li, Xin and Luo, Sai and Zheng, Hui and Yue, Yanzhu and others,
Journal: bioRxiv (2018): 475723
Resources-Application Notes Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy
Authors: Larson, Brad and Banks, Peter
Journal: Experimental Biology (2017)
Authors: Larson, Brad and Banks, Peter
Journal: Experimental Biology (2017)
References
View all 11 references: Citation Explorer
A study of interaction of 7-aminoactinomycin D with DNA by fluorescence correlation spectroscopy
Authors: Kovalev AE, Iakovenko AA, Vekshin NL.
Journal: Biofizika (2004): 1030
Authors: Kovalev AE, Iakovenko AA, Vekshin NL.
Journal: Biofizika (2004): 1030
Multiparametric flow cytometric analysis of biochemical and functional events associated with apoptosis and oncosis using the 7-aminoactinomycin D assay
Authors: Lecoeur H, de Oliveira-Pinto LM, Gougeon ML.
Journal: J Immunol Methods (2002): 81
Authors: Lecoeur H, de Oliveira-Pinto LM, Gougeon ML.
Journal: J Immunol Methods (2002): 81
Phagocytosis of apoptotic cells assessed by flow cytometry using 7-Aminoactinomycin D
Authors: Gaforio JJ, Serrano MJ, Algarra I, Ortega E, Alvarez de Cienfuegos G.
Journal: Cytometry (2002): 8
Authors: Gaforio JJ, Serrano MJ, Algarra I, Ortega E, Alvarez de Cienfuegos G.
Journal: Cytometry (2002): 8
Accessibility of 7-aminoactinomycin D to lymphocyte nuclei after paraformaldehyde fixation
Authors: Linden E, Skoglund P, Rundquist I.
Journal: Cytometry (1997): 92
Authors: Linden E, Skoglund P, Rundquist I.
Journal: Cytometry (1997): 92
High affinity binding of 7-aminoactinomycin D and 4',6-diamidino-2-phenylindole to human neutrophilic granulocytes and lymphocytes
Authors: Loborg H, Linden E, Lonn A, Skoglund P, Rundquist I.
Journal: Cytometry (1995): 296
Authors: Loborg H, Linden E, Lonn A, Skoglund P, Rundquist I.
Journal: Cytometry (1995): 296
Equilibrium binding of DAPI and 7-aminoactinomycin D to chromatin of cultured cells
Authors: Rundquist I., undefined
Journal: Cytometry (1993): 610
Authors: Rundquist I., undefined
Journal: Cytometry (1993): 610
Differential chromatin structure-dependent binding of 7-aminoactinomycin D in normal and malignant bone marrow hematopoietic cells
Authors: Stokke T, Holte H, Smel and EB, Lie SO, Steen HB.
Journal: Cancer Res (1992): 5007
Authors: Stokke T, Holte H, Smel and EB, Lie SO, Steen HB.
Journal: Cancer Res (1992): 5007
Actinomycin D and 7-aminoactinomycin D binding to single-stranded DNA
Authors: Wadkins RM, Jovin TM.
Journal: Biochemistry (1991): 9469
Authors: Wadkins RM, Jovin TM.
Journal: Biochemistry (1991): 9469
Effect of deproteinization on the in situ chromatin staining with 7-aminoactinomycin D
Authors: Poletaev AI, Stepanova NG, Nikitin SM.
Journal: Mol Biol (Mosk) (1988): 1062
Authors: Poletaev AI, Stepanova NG, Nikitin SM.
Journal: Mol Biol (Mosk) (1988): 1062
In vitro and in vivo activation of B-lymphocytes: a flow cytometric study of chromatin structure employing 7-aminoactinomycin D
Authors: Stokke T, Holte H, Steen HB.
Journal: Cancer Res (1988): 6708
Authors: Stokke T, Holte H, Steen HB.
Journal: Cancer Res (1988): 6708