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7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1*

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Physical properties
Molecular weight1270.43
Spectral properties
Extinction coefficient (cm -1 M -1)275001
Excitation (nm)549
Emission (nm)648
Quantum yield0.021
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
7-Amino actinomycin D (7-AAD) is a non-permeant dye that can be used to identify non-viable cells. Cells with damaged plasma membranes or with impaired/no cell metabolism are unable to prevent the dye from entering the cell. Once inside the cell, the dyes bind to intracellular DNA producing highly fluorescent adducts which identify the cells as "non-viable". It is widely used in flow cytometry. 7-AAD is excited by the 488 nm laser line of an argon laser with fluorescence detected above 650 nm. Although the emission intensity of 7-AAD is lower than that of PI, the longer wavelength emission may make it more useful for multiplexing assays in combination with other 488 nm-excited fluorochromes such as FITC and PE.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM78.714 µL393.568 µL787.135 µL3.936 mL7.871 mL
5 mM15.743 µL78.714 µL157.427 µL787.135 µL1.574 mL
10 mM7.871 µL39.357 µL78.714 µL393.568 µL787.135 µL

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Spectral properties

Extinction coefficient (cm -1 M -1)275001
Excitation (nm)549
Emission (nm)648
Quantum yield0.021



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View all 11 references: Citation Explorer
A study of interaction of 7-aminoactinomycin D with DNA by fluorescence correlation spectroscopy
Authors: Kovalev AE, Iakovenko AA, Vekshin NL.
Journal: Biofizika (2004): 1030
Phagocytosis of apoptotic cells assessed by flow cytometry using 7-Aminoactinomycin D
Authors: Gaforio JJ, Serrano MJ, Algarra I, Ortega E, Alvarez de Cienfuegos G.
Journal: Cytometry (2002): 8
Multiparametric flow cytometric analysis of biochemical and functional events associated with apoptosis and oncosis using the 7-aminoactinomycin D assay
Authors: Lecoeur H, de Oliveira-Pinto LM, Gougeon ML.
Journal: J Immunol Methods (2002): 81
Accessibility of 7-aminoactinomycin D to lymphocyte nuclei after paraformaldehyde fixation
Authors: Linden E, Skoglund P, Rundquist I.
Journal: Cytometry (1997): 92
High affinity binding of 7-aminoactinomycin D and 4',6-diamidino-2-phenylindole to human neutrophilic granulocytes and lymphocytes
Authors: Loborg H, Linden E, Lonn A, Skoglund P, Rundquist I.
Journal: Cytometry (1995): 296
Equilibrium binding of DAPI and 7-aminoactinomycin D to chromatin of cultured cells
Authors: Rundquist I., undefined
Journal: Cytometry (1993): 610
Differential chromatin structure-dependent binding of 7-aminoactinomycin D in normal and malignant bone marrow hematopoietic cells
Authors: Stokke T, Holte H, Smel and EB, Lie SO, Steen HB.
Journal: Cancer Res (1992): 5007
Actinomycin D and 7-aminoactinomycin D binding to single-stranded DNA
Authors: Wadkins RM, Jovin TM.
Journal: Biochemistry (1991): 9469
In vitro and in vivo activation of B-lymphocytes: a flow cytometric study of chromatin structure employing 7-aminoactinomycin D
Authors: Stokke T, Holte H, Steen HB.
Journal: Cancer Res (1988): 6708
Effect of deproteinization on the in situ chromatin staining with 7-aminoactinomycin D
Authors: Poletaev AI, Stepanova NG, Nikitin SM.
Journal: Mol Biol (Mosk) (1988): 1062