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7 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
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Application notes
7 Dye qPCR Calibration Plate contains seven spectral calibration plates with seven separate dye standards (TAMRA, SYBR, FAM, JOE, NED, ROX, and VIC). It can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
| VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
| ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
| FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
| SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 498 | 522 | - |
| NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
| JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
| Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 651 | 670 | 2500001 |
| ABY Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 559 | 570 | 1200001 |
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References
View all 50 references: Citation Explorer
Evaluation of the Thermo Scientific SureTect Salmonella Species PCR Assay in a Broad Range of Foods and Select Environmental Surfaces: Pre-Collaborative and Collaborative Study: First Action 2021.02.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Leonte, Ana-Maria and Vandoros, Evangelos J and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022): 167-190
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Leonte, Ana-Maria and Vandoros, Evangelos J and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022): 167-190
Development and Evaluation of a TaqMan Real-Time PCR Assay for the Rapid Detection of Cross-Contamination of RD (Human) and L20B (Mouse) Cell Lines Used in Poliovirus Surveillance.
Authors: Ahmad, Ausaf and Lee, Joo R and Metz, John M and Tang, Xiaoling and Lin, Seh-Ching and Bagarozzi, Dennis A and Petway, David and Herzegh, Owen
Journal: Journal of virological methods (2022): 114354
Authors: Ahmad, Ausaf and Lee, Joo R and Metz, John M and Tang, Xiaoling and Lin, Seh-Ching and Bagarozzi, Dennis A and Petway, David and Herzegh, Owen
Journal: Journal of virological methods (2022): 114354
Evaluation of the Thermo ScientificTM SureTectTMListeria monocytogenes PCR Assay in a Broad Range of Foods and Selected Environmental Surfaces: Pre-Collaborative and Collaborative Study, First Action 2021.05.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Vandoros, Evangelos J and Leonte, Ana-Maria and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022)
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Vandoros, Evangelos J and Leonte, Ana-Maria and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022)
The Comparison of Real-time-PCR-HRM and Microscopy Methods for Detection of Mixed Plasmodium spp. Infections in Laghman Province, Afghanistan.
Authors: Dalimi, Abdolhossein and Mosawi, Sayed Hussain
Journal: Infectious disorders drug targets (2021): 399-404
Authors: Dalimi, Abdolhossein and Mosawi, Sayed Hussain
Journal: Infectious disorders drug targets (2021): 399-404
Clinical evaluation of a multiplex real-time RT-PCR assay for detection of SARS-CoV-2 in individual and pooled upper respiratory tract samples.
Authors: Laverack, Melissa and Tallmadge, Rebecca L and Venugopalan, Roopa and Cronk, Brittany and Zhang, XiuLin and Rauh, Rolf and Saunders, Amy and Nelson, William M and Plocharczyk, Elizabeth and Diel, Diego G
Journal: Archives of virology (2021): 2551-2561
Authors: Laverack, Melissa and Tallmadge, Rebecca L and Venugopalan, Roopa and Cronk, Brittany and Zhang, XiuLin and Rauh, Rolf and Saunders, Amy and Nelson, William M and Plocharczyk, Elizabeth and Diel, Diego G
Journal: Archives of virology (2021): 2551-2561