Calculator
7 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Real-Time PCR (qPCR)
7 Dye qPCR Calibration Plate contains seven spectral calibration plates with seven separate dye standards (TAMRA, SYBR, FAM, JOE, NED, ROX, and VIC). It can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.
Components
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
| VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
| ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
| FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
| SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 498 | 522 | - |
| NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
| JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
| Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 651 | 670 | 2500001 |
| ABY Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 559 | 570 | 1200001 |
Show More (2) | |||
Images
Figure 1. ABI 7500 FAST system spectra for seven different dye standards (TAMRA, VIC, ROX, FAM, SYBR, NED, and JOE).
References
View all 50 references: Citation Explorer
Evaluation of the Thermo Scientific SureTect Salmonella Species PCR Assay in a Broad Range of Foods and Select Environmental Surfaces: Pre-Collaborative and Collaborative Study: First Action 2021.02.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Leonte, Ana-Maria and Vandoros, Evangelos J and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022): 167-190
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Leonte, Ana-Maria and Vandoros, Evangelos J and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022): 167-190
Development and Evaluation of a TaqMan Real-Time PCR Assay for the Rapid Detection of Cross-Contamination of RD (Human) and L20B (Mouse) Cell Lines Used in Poliovirus Surveillance.
Authors: Ahmad, Ausaf and Lee, Joo R and Metz, John M and Tang, Xiaoling and Lin, Seh-Ching and Bagarozzi, Dennis A and Petway, David and Herzegh, Owen
Journal: Journal of virological methods (2022): 114354
Authors: Ahmad, Ausaf and Lee, Joo R and Metz, John M and Tang, Xiaoling and Lin, Seh-Ching and Bagarozzi, Dennis A and Petway, David and Herzegh, Owen
Journal: Journal of virological methods (2022): 114354
Evaluation of the Thermo ScientificTM SureTectTMListeria monocytogenes PCR Assay in a Broad Range of Foods and Selected Environmental Surfaces: Pre-Collaborative and Collaborative Study, First Action 2021.05.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Vandoros, Evangelos J and Leonte, Ana-Maria and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022)
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Vandoros, Evangelos J and Leonte, Ana-Maria and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022)
The Comparison of Real-time-PCR-HRM and Microscopy Methods for Detection of Mixed Plasmodium spp. Infections in Laghman Province, Afghanistan.
Authors: Dalimi, Abdolhossein and Mosawi, Sayed Hussain
Journal: Infectious disorders drug targets (2021): 399-404
Authors: Dalimi, Abdolhossein and Mosawi, Sayed Hussain
Journal: Infectious disorders drug targets (2021): 399-404
Clinical evaluation of a multiplex real-time RT-PCR assay for detection of SARS-CoV-2 in individual and pooled upper respiratory tract samples.
Authors: Laverack, Melissa and Tallmadge, Rebecca L and Venugopalan, Roopa and Cronk, Brittany and Zhang, XiuLin and Rauh, Rolf and Saunders, Amy and Nelson, William M and Plocharczyk, Elizabeth and Diel, Diego G
Journal: Archives of virology (2021): 2551-2561
Authors: Laverack, Melissa and Tallmadge, Rebecca L and Venugopalan, Roopa and Cronk, Brittany and Zhang, XiuLin and Rauh, Rolf and Saunders, Amy and Nelson, William M and Plocharczyk, Elizabeth and Diel, Diego G
Journal: Archives of virology (2021): 2551-2561
TaqMan real time PCR for the Detection of the Gilbert's Syndrome Markers UGT1A1*28; UGT1A1*36 and UGT1A1*37.
Authors: Daprà, Valentina and Alliaudi, Carla and Galliano, Ilaria and Dini, Maddalena and Curcio, Giada Lo and Calvi, Cristina and Archetti, Marialaura and Gavatorta, Martina and Bergallo, Massimiliano
Journal: Molecular biology reports (2021): 4953-4959
Authors: Daprà, Valentina and Alliaudi, Carla and Galliano, Ilaria and Dini, Maddalena and Curcio, Giada Lo and Calvi, Cristina and Archetti, Marialaura and Gavatorta, Martina and Bergallo, Massimiliano
Journal: Molecular biology reports (2021): 4953-4959
Rapid and Safe Detection of SARS-CoV-2 and Influenza Virus RNA Using Onsite Quantitative PCR Diagnostic Testing From Clinical Specimens Collected in Molecular Transport Medium.
Authors: Daum, Luke T and Fischer, Gerald W
Journal: The journal of applied laboratory medicine (2021): 1409-1416
Authors: Daum, Luke T and Fischer, Gerald W
Journal: The journal of applied laboratory medicine (2021): 1409-1416
Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay.
Authors: Potter, Robert F and Abro, Brooj and Eby, Charles S and Burnham, Carey-Ann D and Anderson, Neil W and Parikh, Bijal A
Journal: Diagnostic microbiology and infectious disease (2021): 115387
Authors: Potter, Robert F and Abro, Brooj and Eby, Charles S and Burnham, Carey-Ann D and Anderson, Neil W and Parikh, Bijal A
Journal: Diagnostic microbiology and infectious disease (2021): 115387
Validation of the Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay for the Detection of Staphylococcus aureus in Dairy Matrices: AOAC Performance Tested MethodsSM 052101.
Authors: Evans, Katharine and Faulds, Nikki and Crabtree, David and Hughes, Annette and Sohier, Daniele and Manthe, Craig and Hahs, Matthew and Heikkinen, Pauliina and Hurskainen, Emmi and Koch, Kateland and Thompson, Wesley and Bastin, Benjamin and Benzinger, M Joseph
Journal: Journal of AOAC International (2021)
Authors: Evans, Katharine and Faulds, Nikki and Crabtree, David and Hughes, Annette and Sohier, Daniele and Manthe, Craig and Hahs, Matthew and Heikkinen, Pauliina and Hurskainen, Emmi and Koch, Kateland and Thompson, Wesley and Bastin, Benjamin and Benzinger, M Joseph
Journal: Journal of AOAC International (2021)
Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2.
Authors: Moore, Nicholas M and Li, Haiying and Schejbal, Debra and Lindsley, Jennifer and Hayden, Mary K
Journal: Journal of clinical microbiology (2020)
Authors: Moore, Nicholas M and Li, Haiying and Schejbal, Debra and Lindsley, Jennifer and Hayden, Mary K
Journal: Journal of clinical microbiology (2020)
Application notes
Sealing PCR Plates
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?