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7-Ethoxy-4-trifluoromethylcoumarin *CAS 115453-82-2*

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Physical properties
Molecular weight258.19
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins
Molecular weight
This fluorogenic coumarin ether is widely used for monitoring P450 activities in cell extracts and solutions. Cytochrome P450 oxidase (CYP450) is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Ethoxy-4-trifluoromethylcoumarin *CAS 115453-82-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM387.312 µL1.937 mL3.873 mL19.366 mL38.731 mL
5 mM77.462 µL387.312 µL774.623 µL3.873 mL7.746 mL
10 mM38.731 µL193.656 µL387.312 µL1.937 mL3.873 mL

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View all 1 citations: Citation Explorer
Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system
Authors: Richter, M and Fairhall, EA and Hoffmann, SA and Tr&ouml;bs, S and Kn&ouml;spel, F and Probert, PME and Oakley, F and Stroux, A and Wright, MC and Zeilinger, K
Journal: Toxicology Research (2016): 278--290


View all 57 references: Citation Explorer
Induction of cytochrome P450 3A4 and P-glycoprotein by the isoxazolyl-penicillin antibiotic flucloxacillin
Authors: Huwyler J, Wright MB, Gutmann H, Drewe J.
Journal: Curr Drug Metab (2006): 119
Luminogenic cytochrome P450 assays
Authors: Cali JJ, Ma D, Sobol M, Simpson DJ, Frackman S, Good TD, Daily WJ, Liu D.
Journal: Expert Opin Drug Metab Toxicol (2006): 629
Kinetics and thermodynamics of ligand binding by cytochrome P450 3A4
Authors: Isin EM, Guengerich FP.
Journal: J Biol Chem (2006): 9127
Resolution of multiple substrate binding sites in cytochrome P450 3A4: the stoichiometry of the enzyme-substrate complexes probed by FRET and Job's titration
Authors: Fern, undefined and o H, Halpert JR, Davydov DR.
Journal: Biochemistry (2006): 4199
Binding of 7-methoxy-4-(aminomethyl)-coumarin to wild-type and W128F mutant cytochrome P450 2D6 studied by time-resolved fluorescence spectroscopy
Authors: Stortelder A, Keizers PH, Oostenbrink C, De Graaf C, De Kruijf P, Vermeulen NP, Gooijer C, Comm and eur JN, Van der Zwan G.
Journal: Biochem J (2006): 635
Time-resolved fluorescence studies of heterotropic ligand binding to cytochrome P450 3A4
Authors: Lampe JN, Atkins WM.
Journal: Biochemistry (2006): 12204
Methoxy-resorufin ether as an electrochemically active biological probe for cytochrome P450 O-demethylation
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Cytochrome P450 (CYP) inhibition screening: comparison of three tests
Authors: Turpeinen M, Korhonen LE, Tolonen A, Uusitalo J, Juvonen R, Raunio H, Pelkonen O.
Journal: Eur J Pharm Sci (2006): 130
Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction
Authors: Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD.
Journal: J Biol Chem (2006): 5973
Ginkgo biloba does not alter clearance of flurbiprofen, a cytochrome P450-2C9 substrate
Authors: Greenblatt DJ, von Moltke LL, Luo Y, Perloff ES, Horan KA, Bruce A, Reynolds RC, Harmatz JS, Avula B, Khan IA, Goldman P.
Journal: J Clin Pharmacol (2006): 214