7-Ethoxy-4-trifluoromethylcoumarin *CAS 115453-82-2*

7-Ethoxy-4-trifluoromethylcoumarin

CAS 115453-82-2

This fluorogenic coumarin ether is widely used for monitoring P450 activities in cell extracts and solutions. Cytochrome P450 oxidase (CYP450) is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site.

Protocol

SDS

Catalog	Size	Price	Quantity
15005	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Ethoxy-4-trifluoromethylcoumarin *CAS 115453-82-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	387.312 µL	1.937 mL	3.873 mL	19.366 mL	38.731 mL
5 mM	77.462 µL	387.312 µL	774.623 µL	3.873 mL	7.746 mL
10 mM	38.731 µL	193.656 µL	387.312 µL	1.937 mL	3.873 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	258.19 g/mol	=	mL	x	mM	=	mmol

References

View all 57 references: Citation Explorer

Methoxy-resorufin ether as an electrochemically active biological probe for cytochrome P450 O-demethylation

Authors:

Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.

Journal:

Bioelectrochemistry (2006): 67

Comparison of cytochrome P450 inhibition assays for drug discovery using human liver microsomes with LC-MS, rhCYP450 isozymes with fluorescence, and double cocktail with LC-MS

Authors:

Di L, Kerns EH, Li SQ, Carter GT.

Journal:

Int J Pharm. (2006)

Ginkgo biloba does not alter clearance of flurbiprofen, a cytochrome P450-2C9 substrate

Authors:

Greenblatt DJ, von Moltke LL, Luo Y, Perloff ES, Horan KA, Bruce A, Reynolds RC, Harmatz JS, Avula B, Khan IA, Goldman P.

Journal:

J Clin Pharmacol (2006): 214

Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction

Authors:

Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD.

Journal:

J Biol Chem (2006): 5973

Cytochrome P450 (CYP) inhibition screening: comparison of three tests

Authors:

Turpeinen M, Korhonen LE, Tolonen A, Uusitalo J, Juvonen R, Raunio H, Pelkonen O.

Journal:

Eur J Pharm Sci (2006): 130

Physical properties

Molecular weight	258.19
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	115453-82-2

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits

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