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7-Hydroxy-4-methylcoumarin-3-acetic acid *CAS 5852-10-8*

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Physical properties
Molecular weight234.21
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Coumarins
CAS
5852-10-8
Molecular weight
234.21
7-Hydroxy-4-methylcoumarin-3-acetic acid is a blue fluorophore that has pH-dependent and environment-sensitive fluorescence. This coumarin is increasingly used to label peptides, nucleotides and carbohydrates.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin-3-acetic acid *CAS 5852-10-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM426.967 µL2.135 mL4.27 mL21.348 mL42.697 mL
5 mM85.393 µL426.967 µL853.935 µL4.27 mL8.539 mL
10 mM42.697 µL213.484 µL426.967 µL2.135 mL4.27 mL

Molarity calculator

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References


View all 1 references: Citation Explorer
Sensitivity enhancement of fluorescent pH indicators using pH-dependent energy transfer
Authors: Gabor G, et al.
Journal: Anal Chim Acta (1995): 131