7-Hydroxy-4-methylcoumarin-3-acetic acid, succinimidyl ester

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Telephone	1-800-990-8053
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Physical properties

Molecular weight	331.28
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

331.28

7-Hydroxy-4-methylcoumarin-3-acetic acid, SE is an amine-reactive blue fluorophore that has pH-dependent and environment-sensitive fluorescence. It is widely used for preparing bioconjugates of blue fluorescence.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxy-4-methylcoumarin-3-acetic acid, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	301.859 µL	1.509 mL	3.019 mL	15.093 mL	30.186 mL
5 mM	60.372 µL	301.859 µL	603.719 µL	3.019 mL	6.037 mL
10 mM	30.186 µL	150.93 µL	301.859 µL	1.509 mL	3.019 mL