Name: 7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1*
Brand: AAT Bioquest
SKU: 549
Availability: InStock

7-Hydroxycoumarin-3-carboxylic acid

CAS 779-27-1

7-Hydroxycoumarin-3-carboxylic acid is a blue fluorophores for labeling proteins and nucleic acids mostly through the in situ formation of its succinimidyl ester catalyzed by EDAC.

Protocol

COA

Catalog	Size	Price	Quantity
549	25 mg	Price
550	250 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	485.084 µL	2.425 mL	4.851 mL	24.254 mL	48.508 mL
5 mM	97.017 µL	485.084 µL	970.167 µL	4.851 mL	9.702 mL
10 mM	48.508 µL	242.542 µL	485.084 µL	2.425 mL	4.851 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	206.15 g/mol	=	mL	x	mM	=	mmol

References

View all 16 references: Citation Explorer

In vitro anti-tumour and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma cell lines

Authors:

Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.

Journal:

Cancer Lett. (2006)

Effects of iron on Vitamin C/copper-induced hydroxyl radical generation in bicarbonate-rich water

Authors:

Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.

Journal:

Free Radic Res (2005): 565

Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs

Authors:

Hashidoko Y, Tanaka T, Tahara S.

Journal:

Biosci Biotechnol Biochem (2001): 2604

A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography

Authors:

Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.

Journal:

Biol Pharm Bull (1999): 333

Flow injection analysis of binding reaction between fluorescent lectin and cells

Authors:

Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.

Journal:

Anal Biochem (1999): 230

Physical properties

Molecular weight	206.15
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.0815
Excitation (nm)	352
Emission (nm)	407

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	779-27-1

Documents

Protocol
Safety Data Sheet (Catalog 550)
Safety Data Sheet (Catalog 549)
Certificate of Analysis

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Page updated on October 27, 2025