Name: 7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 134471-24-2*
Brand: AAT Bioquest
SKU: 551
Price: 115 USD
Availability: InStock

7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester

CAS 134471-24-2

7-Hydroxycoumarin-3-carboxylic acid, SE is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. It is a blue fluorescent dyes for labeling proteins and nucleic acids. This coumarin is also increasingly used to label peptides, nucleotides and carbohydrates.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
551	50 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 134471-24-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	329.783 µL	1.649 mL	3.298 mL	16.489 mL	32.978 mL
5 mM	65.957 µL	329.783 µL	659.565 µL	3.298 mL	6.596 mL
10 mM	32.978 µL	164.891 µL	329.783 µL	1.649 mL	3.298 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	303.23 g/mol	=	mL	x	mM	=	mmol

References

View all 16 references: Citation Explorer

In vitro anti-tumour and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma cell lines

Authors:

Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.

Journal:

Cancer Lett. (2006)

Effects of iron on Vitamin C/copper-induced hydroxyl radical generation in bicarbonate-rich water

Authors:

Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.

Journal:

Free Radic Res (2005): 565

Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs

Authors:

Hashidoko Y, Tanaka T, Tahara S.

Journal:

Biosci Biotechnol Biochem (2001): 2604

A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography

Authors:

Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.

Journal:

Biol Pharm Bull (1999): 333

Flow injection analysis of binding reaction between fluorescent lectin and cells

Authors:

Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.

Journal:

Anal Biochem (1999): 230

Physical properties

Molecular weight	303.23
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.0815
Excitation (nm)	352
Emission (nm)	407

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	134471-24-2

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