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7-Hydroxycoumarin-4-acetic acid *CAS 6950-82-9*

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Physical properties
Molecular weight220.18
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins
Molecular weight
7-Hydroxycoumarin-4-acetic acid is used for labeling peptides and proteins to give blue fluorescence. It can also be used for labeling other biological molecules.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-4-acetic acid *CAS 6950-82-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM454.174 µL2.271 mL4.542 mL22.709 mL45.417 mL
5 mM90.835 µL454.174 µL908.348 µL4.542 mL9.083 mL
10 mM45.417 µL227.087 µL454.174 µL2.271 mL4.542 mL

Molarity calculator

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View all 5 references: Citation Explorer
Stopped-flow kinetic analysis of long-chain fatty acid dissociation from bovine serum albumin
Authors: Demant EJ, Richieri GV, Kleinfeld AM.
Journal: Biochem J (2002): 809
Continuous recording of long-chain acyl-coenzyme a synthetase activity using fluorescently labeled bovine serum albumin
Authors: Demant EJ, Nystrom BT.
Journal: Anal Biochem (2001): 38
Bovine serum albumin-(7-hydroxycoumarin-4-acetic acid) complex: applications to the fluorometric measurement of fatty acid concentrations
Authors: Demant EJ., undefined
Journal: Anal Biochem (1999): 366
Covalent complexes between serum albumin and 7-hydroxycoumarin-4-acetic acid: synthesis and applications in the spectrophotometric detection of long-chain fatty acids
Authors: Demant EJ., undefined
Journal: Biochim Biophys Acta (1996): 43
Structure-activity relationship of coumarins in xanthine oxidase inhibition
Authors: Chang WS, Chiang HC.
Journal: Anticancer Res (1995): 1969