7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*

7-Methoxycoumarin-3-carboxylic acid

CAS 20300-59-8

7-Methoxycoumarin-3-carboxylic acid is used for HPLC derivatization. It is also used to develop FRET probes for analyzing protease activities.

Protocol

COA

Catalog	Size	Price	Quantity
560	1 g	Price
561	5 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	454.174 µL	2.271 mL	4.542 mL	22.709 mL	45.417 mL
5 mM	90.835 µL	454.174 µL	908.348 µL	4.542 mL	9.083 mL
10 mM	45.417 µL	227.087 µL	454.174 µL	2.271 mL	4.542 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	220.18 g/mol	=	mL	x	mM	=	mmol

References

View all 3 references: Citation Explorer

Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence

Authors:

Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.

Journal:

J Pept Sci (2005): 153

An alternative quenched fluorescence substrate for Pz-peptidase

Authors:

Tisljar U, et al.

Journal:

Anal Biochem (1990): 112-5

Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection

Authors:

Mita H, Yasueda H, Hayakawa T, Shida T.

Journal:

Anal Biochem (1989): 131

Physical properties

Molecular weight	220.18
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.171
Excitation (nm)	355
Emission (nm)	405

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	20300-59-8

Documents

Protocol
Safety Data Sheet (Catalog 560)
Safety Data Sheet (Catalog 561)
Certificate of Analysis

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Page updated on October 21, 2025