7-Methoxycoumarin-3-carboxylic acid CAS 20300-59-8

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Physical properties

Molecular weight	220.18
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.171
Excitation (nm)	355
Emission (nm)	405

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	454.174 µL	2.271 mL	4.542 mL	22.709 mL	45.417 mL
5 mM	90.835 µL	454.174 µL	908.348 µL	4.542 mL	9.083 mL
10 mM	45.417 µL	227.087 µL	454.174 µL	2.271 mL	4.542 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.171
Excitation (nm)	355
Emission (nm)	405

Product Family

Name	Excitation (nm)	Emission (nm)	Correction Factor (280 nm)
7-Hydroxycoumarin-3-carboxylic acid CAS 779-27-1	352	407	0.0815

Images

Figure 1. Chemical structure for 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*

References

View all 3 references: Citation Explorer

Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153

An alternative quenched fluorescence substrate for Pz-peptidase
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5

Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131