7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*
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Physical properties
Molecular weight | 220.18 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.171 |
Excitation (nm) | 355 |
Emission (nm) | 405 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 454.174 µL | 2.271 mL | 4.542 mL | 22.709 mL | 45.417 mL |
5 mM | 90.835 µL | 454.174 µL | 908.348 µL | 4.542 mL | 9.083 mL |
10 mM | 45.417 µL | 227.087 µL | 454.174 µL | 2.271 mL | 4.542 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.171 |
Excitation (nm) | 355 |
Emission (nm) | 405 |
Product Family
Name | Excitation (nm) | Emission (nm) | Correction Factor (280 nm) |
7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1* | 352 | 407 | 0.0815 |
References
View all 3 references: Citation Explorer
Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
An alternative quenched fluorescence substrate for Pz-peptidase
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Application notes
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A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets