7-Methoxycoumarin-3-carboxylic acid CAS 20300-59-8

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Physical properties

Molecular weight	220.18
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.171
Excitation (nm)	355
Emission (nm)	405

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

20300-59-8

Molecular weight

220.18

Correction Factor (280 nm)

0.171

Excitation (nm)

355

Emission (nm)

405

7-Methoxycoumarin-3-carboxylic acid is used for HPLC derivatization. It is also used to develop FRET probes for analyzing protease activities.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	454.174 µL	2.271 mL	4.542 mL	22.709 mL	45.417 mL
5 mM	90.835 µL	454.174 µL	908.348 µL	4.542 mL	9.083 mL
10 mM	45.417 µL	227.087 µL	454.174 µL	2.271 mL	4.542 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.171
Excitation (nm)	355
Emission (nm)	405

Product Family

Name	Excitation (nm)	Emission (nm)	Correction Factor (280 nm)
7-Hydroxycoumarin-3-carboxylic acid CAS 779-27-1	352	407	0.0815

Images

Figure 1. Chemical structure for 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*

References

View all 3 references: Citation Explorer

Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153

An alternative quenched fluorescence substrate for Pz-peptidase
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5

Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131