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7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 150321-92-9*

7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester is an excellent amino-reactive tag that has strong blue fluorescence. It is used to label peptides, nucleotides. It is also used to label cell membranes although its fluorescence wavelength is quite short.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 150321-92-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM315.209 µL1.576 mL3.152 mL15.76 mL31.521 mL
5 mM63.042 µL315.209 µL630.418 µL3.152 mL6.304 mL
10 mM31.521 µL157.604 µL315.209 µL1.576 mL3.152 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Correction Factor (280 nm)
7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 134471-24-2*3524070.0815

References

View all 3 references: Citation Explorer
Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
An alternative quenched fluorescence substrate for Pz-peptidase
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Page updated on November 12, 2024

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Physical properties

Molecular weight

317.25

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.171

Excitation (nm)

355

Emission (nm)

405

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

150321-92-9
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