7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 150321-92-9*

7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester

CAS 150321-92-9

7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester is an excellent amino-reactive tag that has strong blue fluorescence. It is used to label peptides, nucleotides. It is also used to label cell membranes although its fluorescence wavelength is quite short.

Protocol

SDS

Catalog	Size	Price	Quantity
563	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester *CAS 150321-92-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	315.209 µL	1.576 mL	3.152 mL	15.76 mL	31.521 mL
5 mM	63.042 µL	315.209 µL	630.418 µL	3.152 mL	6.304 mL
10 mM	31.521 µL	157.604 µL	315.209 µL	1.576 mL	3.152 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	317.25 g/mol	=	mL	x	mM	=	mmol

References

View all 3 references: Citation Explorer

Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence

Authors:

Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.

Journal:

J Pept Sci (2005): 153

An alternative quenched fluorescence substrate for Pz-peptidase

Authors:

Tisljar U, et al.

Journal:

Anal Biochem (1990): 112-5

Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection

Authors:

Mita H, Yasueda H, Hayakawa T, Shida T.

Journal:

Anal Biochem (1989): 131

Physical properties

Molecular weight	317.25
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.171
Excitation (nm)	355
Emission (nm)	405

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	150321-92-9

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Oligonucleotide Labeling Reagents
Brochure: Peptide Labeling Reagents

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