5-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer ]

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Additional ordering information

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Physical properties

Molecular weight	509.37
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.31
Correction Factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	76000
Excitation (nm)	498
Emission (nm)	526

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

509.37

Correction Factor (260 nm)

0.31

Correction Factor (280 nm)

0.12

Extinction coefficient (cm ^-1 M ^-1)

76000

Excitation (nm)

498

Emission (nm)

526

5-OG488 succinimidyl ester is the same molecule to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer (Invitrogen #O6147, Oregon Green® is the Trademark of Invitrogen). This fluorinated analog of fluorescein has higher photostability and a lower pKa ~ 4.7 while 5-FAM has pKa ~6.4. The decreased pKa of 5-OG488 makes its fluorescence essentially pH insensitive in the physiological pH range. This amine-reactive 5-OG488 succinimidyl ester can be used to make bright green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. It is an excellent replacement to 5-FITC that has severe pH dependence and low photostability.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer ] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	196.321 µL	981.605 µL	1.963 mL	9.816 mL	19.632 mL
5 mM	39.264 µL	196.321 µL	392.642 µL	1.963 mL	3.926 mL
10 mM	19.632 µL	98.16 µL	196.321 µL	981.605 µL	1.963 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.31
Correction Factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	76000
Excitation (nm)	498
Emission (nm)	526

Images

Figure 1. Chemical structure for 5-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer ]

Citations

View all 1 citations: Citation Explorer

Quantification of Fundamental Weak Base and Ion Permeabilities in the Acidic pH Region Utilizing Conjugated Oregon Green in Liposome-Based Assays
Authors: Barta, Thomas and Seiser, Lorenz and Horner, Andreas
Journal: Advanced Sensor Research (2023): 2200097

References

View all 14 references: Citation Explorer

Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579

Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506

A mismatch-selective bifunctional rhodium-Oregon Green conjugate: a fluorescent probe for mismatched DNA
Authors: Zeglis BM, Barton JK.
Journal: J Am Chem Soc (2006): 5654

The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581

Collagen-binding adhesion protein 35-Oregon Green 488
Authors: Cheng KT, Van Z and voort M., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)

Cetuximab-Oregon Green 488
Authors: , undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD) (2004)

Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions
Authors: Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alex and er Ross JB., undefined
Journal: Anal Biochem (2002): 18

Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151