Name: 5-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer ]
Brand: AAT Bioquest
SKU: 710
Price: 232 USD
Availability: InStock

5-OG488 succinimidyl ester

equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer

5-OG488 succinimidyl ester is the same molecule to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer (Invitrogen #O6147, Oregon Green® is the Trademark of Invitrogen). This fluorinated analog of fluorescein has higher photostability and a lower pKa ~ 4.7 while 5-FAM has pKa ~6.4. The decreased pKa of 5-OG488 makes its fluorescence essentially pH insensitive in the physiological pH range. This amine-reactive 5-OG488 succinimidyl ester can be used to make bright green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. It is an excellent replacement to 5-FITC that has severe pH dependence and low photostability.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
710	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-OG488 succinimidyl ester [equivalent to Oregon Green® 488 carboxylic acid, succinimidyl ester, 5-isomer ] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	196.321 µL	981.605 µL	1.963 mL	9.816 mL	19.632 mL
5 mM	39.264 µL	196.321 µL	392.642 µL	1.963 mL	3.926 mL
10 mM	19.632 µL	98.16 µL	196.321 µL	981.605 µL	1.963 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	509.37 g/mol	=	mL	x	mM	=	mmol

References

View all 14 references: Citation Explorer

Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound

Authors:

Lee JS, Feijen J.

Journal:

J Control Release (2012): 312

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1

Authors:

Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.

Journal:

Methods Mol Biol (2011): 377

Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes

Authors:

Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.

Journal:

Invest Ophthalmol Vis Sci (2008): 4506

Trans-scleral permeability of Oregon green 488

Authors:

Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.

Journal:

J Ocul Pharmacol Ther (2008): 579

The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes

Authors:

Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.

Journal:

Org Lett (2006): 581

Physical properties

Molecular weight	509.37
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.31
Correction factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	76000
Excitation (nm)	498
Emission (nm)	526

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025