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AA-dUTP
Aminoallyl dUTP sodium salt; 4 mM in TE Buffer (pH 7.5); CAS 936327-10-5
The amine-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminoallyl dUTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminoallyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled uridine triphosphates that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.
Chemical structure for AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in TE Buffer (pH 7.5)* *CAS 936327-10-5*
Chemical structure for AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in TE Buffer (pH 7.5)* *CAS 936327-10-5*
protocol
Protocol
COO
COA
CatalogSize
Price
Quantity
170041 umole
Price
170052.5 umoles
Price
 
Physical properties
Molecular weight589.17
SolventWater
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
CAS936327-10-5
Documents
Protocol
Safety Data Sheet (Catalog 17004)
Safety Data Sheet (Catalog 17005)
Certificate of Origin
Certificate of Analysis
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Page updated on October 18, 2025