AA-UTP [Aminoallyl UTP sodium salt] *4 mM in TE buffer* *CAS 112131-73-4*
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Physical properties
Molecular weight | 605.16 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Overview | SDSProtocol |
See also: Fluorescent in situ hybridization (FISH), Nucleic Acid Building Blocks, RNA Purification & Analysis
CAS 112131-73-4 | Molecular weight 605.16 |
The amine-modified uridine 5'-triphosphates (such as aminoallyl-UTP) can be used to produce amine-containing RNA by conventional enzymatic incorporation methods such as transcription, nick translation, random primed labeling, or PCR. Aminoallyl UTP can be readily incorporated into RNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminoallyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled uridine triphosphates that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of AA-UTP [Aminoallyl UTP sodium salt] *4 mM in TE buffer* *CAS 112131-73-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 165.246 µL | 826.228 µL | 1.652 mL | 8.262 mL | 16.525 mL |
5 mM | 33.049 µL | 165.246 µL | 330.491 µL | 1.652 mL | 3.305 mL |
10 mM | 16.525 µL | 82.623 µL | 165.246 µL | 826.228 µL | 1.652 mL |
Molarity calculator
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References
View all 3 references: Citation Explorer
Fluorescent labelling of cRNA for microarray applications
Authors: t Hoen PA, de Kort F, van Ommen GJ, den Dunnen JT.
Journal: Nucleic Acids Res (2003): e20
Authors: t Hoen PA, de Kort F, van Ommen GJ, den Dunnen JT.
Journal: Nucleic Acids Res (2003): e20
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Sequence-specific scission of DNA by the chemical nuclease activity of 1,10-phenanthroline-copper(I) targeted by RNA
Authors: Chen CB, Gorin MB, Sigman DS.
Journal: Proc Natl Acad Sci U S A (1993): 4206
Authors: Chen CB, Gorin MB, Sigman DS.
Journal: Proc Natl Acad Sci U S A (1993): 4206
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