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AA-UTP
Aminoallyl UTP sodium salt; 4 mM in TE buffer; CAS 112131-73-4
The amine-modified uridine 5'-triphosphates (such as aminoallyl-UTP) can be used to produce amine-containing RNA by conventional enzymatic incorporation methods such as transcription, nick translation, random primed labeling, or PCR. Aminoallyl UTP can be readily incorporated into RNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminoallyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled uridine triphosphates that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.
Chemical structure for AA-UTP [Aminoallyl UTP sodium salt] *4 mM in TE buffer* *CAS 75221-88-4*
Chemical structure for AA-UTP [Aminoallyl UTP sodium salt] *4 mM in TE buffer* *CAS 75221-88-4*
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
17021250 uL
Price
 
Physical properties
Molecular weight605.16
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
CAS112131-73-4
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Page updated on October 14, 2025