AATOM™ 390 amine

AATOM™ 390 amine is a carbonyl-reactive, blue fluorescent dye with an excitation/emission of 390/475 nm that is used for FACS and FISH applications.

Versatile Conjugation: Efficiently labels carbonyl groups (e.g., aldehyde, ketone, and carboxy groups); also an amine donor for enzymatic transamination labeling
High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
Large Stokes Shift: Enables effective signal separation, reducing background interference

The chemical structure of AATOM™ 390 amine.

Datasheet

SDS

Catalog	Size	Price	Quantity
70208	1 mg	Price

Overview

AATOM™ 390 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, good photostability, and low molecular weight. It exhibits moderate hydrophilicity and is optimally excited within the 360–410 nm range, with a mercury arc lamp (emission lines at 365 nm and 405 nm) serving as an effective excitation source. This dye is well-suited for applications in single-molecule detection and advanced high-resolution microscopy techniques, including PALM, dSTORM, and STED. Additionally, AATOM™ 390 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other diverse biological assays.

AATOM™ 390 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 390 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	200.188 µL	1.001 mL	2.002 mL	10.009 mL	20.019 mL
5 mM	40.038 µL	200.188 µL	400.376 µL	2.002 mL	4.004 mL
10 mM	20.019 µL	100.094 µL	200.188 µL	1.001 mL	2.002 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	499.53 g/mol	=	mL	x	mM	=	mmol

References

View all 7 references: Citation Explorer

The new live imagers MitoMM1/2 for mitochondrial visualization.

Authors:

Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi

Journal:

Biochemical and biophysical research communications (2021): 50-54

DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission.

Authors:

Ucar, Hülya and Wagenknecht, Hans-Achim

Journal:

Chemical science (2021): 10048-10053

Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.

Authors:

Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther

Journal:

Scientific reports (2021): 14364

Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.

Authors:

Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin

Journal:

Bioanalysis (2019): 1917-1925

The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling.

Authors:

Babcock, Jeremiah J and Brancaleon, Lorenzo

Journal:

Biopolymers (2015): 285-95

Physical properties

Molecular weight	499.53
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.46
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	24000
Excitation (nm)	390
Emission (nm)	476
Quantum yield	.90

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on April 28, 2026