AATOM™ 425 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, excellent photostability, and low molecular weight. It exhibits moderate hydrophilicity and is optimally excited in the 405-455 nm wavelength range. These properties make AATOM™ 425 particularly suitable for applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. Additionally, AATOM™ 425 is well-suited for use in flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

The maleimide derivative of AATOM™ 425 is widely used for labeling biomolecules with free thiol (SH) groups, including peptides and oligonucleotides. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.