AATOM™ 488 Tetrazine

AATOM™ 488 tetrazine is a TCO-reactive, green-fluorescent dye with an excitation/emission of 499/520 nm that is used for FACS and FISH applications.

Click Chemistry Conjugation: Provides rapid and copper-free labeling of TCO-modified biomolecules
Superior Brightness & Stability: Delivers robust, steady fluorescence for sensitive applications
High Hydrophilicity: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

The chemical structure of ATTO 488 tetrazine.

Datasheet

SDS

Catalog	Size	Price	Quantity
2800	1 mg	Price

Overview

AATOM™ 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480–515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. AATOM™ 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.

AATOM™ 488 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 488 Tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	114.416 µL	572.082 µL	1.144 mL	5.721 mL	11.442 mL
5 mM	22.883 µL	114.416 µL	228.833 µL	1.144 mL	2.288 mL
10 mM	11.442 µL	57.208 µL	114.416 µL	572.082 µL	1.144 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	874 g/mol	=	mL	x	mM	=	mmol

References

View all 32 references: Citation Explorer

Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.

Authors:

Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman

Journal:

Photochemistry and photobiology (2024): 561-572

Analysis of photon trajectories from diffusing single molecules.

Authors:

Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung

Journal:

The Journal of chemical physics (2023)

Curvature preference of cubic CsPbBr3 quantum dots embedded onto phospholipid bilayer membranes.

Authors:

Chairil, Ricki and Malmstadt, Noah

Journal:

Soft matter (2023): 3966-3974

Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.

Authors:

Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V

Journal:

Membranes (2023)

Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate).

Authors:

Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi

Journal:

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184

Physical properties

Molecular weight	874.00
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.22
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.80

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on May 5, 2026