Name: AATOM™ 532 alkyne
Brand: AAT Bioquest
SKU: 2825
Price: 118 USD
Availability: InStock

AATOM™ 532 alkyne

AATOM™ 532 alkyne is an azide-reactive, yellow fluorescent dye with an excitation/emission of 531/552 nm that is used for FACS and FISH applications.

Click Chemistry: enables efficient, bioorthogonal labeling of azide-functionalized biomolecules
High Quantum Yield & Photostability: ensures intense fluorescence signals for reliable detection and imaging
Excellent Hydrophilicity: Prevents aggregation and enhances signal clarity in live-cell applications
Advanced Imaging Performance: Suitable for single-molecule detection and super-resolution techniques, including SIM and STED

The chemical structure of AATOM™ 532 alkyne.

Datasheet

SDS

Catalog	Size	Price	Quantity
2825	1 mg	Price

Overview

AATOM™ 532 is a rhodamine-based fluorescent dye notable for its high molar absorptivity and fluorescence quantum yield (0.90), providing robust signal intensity in fluorescence applications. Its combination of photostability, aqueous solubility, and sufficient Stokes shift makes it suitable for single-molecule detection and high-resolution microscopy techniques, including SIM and STED microscopy. AATOM™ 532 is also effective in flow cytometry, FISH, and various biological assays, offering flexibility for diverse fluorescence-based experimental protocols. The dye is optimally excited within the 515–545 nm range, with a frequency-doubled Nd:YAG laser at 532 nm serving as an ideal excitation source.

The alkyne derivative of AATOM™ 532 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 532 alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	127.558 µL	637.788 µL	1.276 mL	6.378 mL	12.756 mL
5 mM	25.512 µL	127.558 µL	255.115 µL	1.276 mL	2.551 mL
10 mM	12.756 µL	63.779 µL	127.558 µL	637.788 µL	1.276 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	783.96 g/mol	=	mL	x	mM	=	mmol

References

View all 5 references: Citation Explorer

Extending the Palette of Green Coumarin Photocages - Oligonucleotide Fragmentation and Superior 5'-Caps.

Authors:

Kaufmann, Janik and Wolf, Jonas and Heckel, Alexander

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202300390

DNA-Programmed Lipid Nanoreactors for Synthesis of Carbohydrate Mimetics by Fusion of Aqueous Sub-attoliter Compartments.

Authors:

Tian, Xinwei and Risgaard, Nikolaj Alexander and Löffler, Philipp M G and Vogel, Stefan

Journal:

Journal of the American Chemical Society (2023)

Fluorescence Lifetime Imaging Microscopy (FLIM) of Intracellular Transport by Means of Doubly Labelled siRNA Architectures.

Authors:

Doll, Larissa and Lackner, Jens and Rönicke, Franziska and Nienhaus, Gerd Ulrich and Wagenknecht, Hans-Achim

Journal:

Chembiochem : a European journal of chemical biology (2021): 2561-2567

Click chemistry for the conservation of cellular structures and fluorescent proteins: ClickOx.

Authors:

Löschberger, Anna and Niehörster, Thomas and Sauer, Markus

Journal:

Biotechnology journal (2014): 693-7

Clickable degradable aliphatic polyesters via copolymerization with alkyne epoxy esters: synthesis and postfunctionalization with organic dyes.

Authors:

Teske, Nele S and Voigt, Julia and Shastri, V Prasad

Journal:

Journal of the American Chemical Society (2014): 10527-33

Physical properties

Molecular weight	783.96
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.22
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	115000
Excitation (nm)	532
Emission (nm)	551
Quantum yield	0.90

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on May 5, 2026