AATOM™ 532 is a rhodamine-based fluorescent dye characterized by its strong absorption and exceptional fluorescence quantum yield (0.90). It demonstrates good photostability along with excellent water solubility, and features a sufficient Stokes shift (Ex/Em = 531/552). AATOM™ 532 is highly suitable for single-molecule detection and high-resolution microscopy techniques such as SIM and STED. Additionally, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research. It is optimally excited within the 515-545 nm range, with the 532 nm output of a frequency-doubled Nd:YAG laser serving as an ideal excitation source.

The maleimide derivative of AATOM™ 532 is widely used for labeling biomolecules with free thiol (SH) groups, including peptides and oligonucleotides. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.