AATOM™ 565 Acid, 5-Isomer

AATOM™ 565 acid 5 Isomer is a non-reactive, orange fluorescent dye with an excitation/emission of 562/589 nm that is used for FACS and FISH applications.

High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
Enhanced Signal Separation: Provides effective signal clarity by minimizing background interference
Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as AATOM™ 565 Acid, 5-Isomer, can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.

Datasheet

SDS

Catalog	Size	Price	Quantity
2826	25 mg	Price

Overview

AATOM™ 565 Acid, 5-Isomer is an amine-reactive rhodamine dye commonly used for labeling peptides, proteins, and other amine-containing molecules, such as amino-modified oligonucleotides. In the presence of a coupling reagent such as EDC, this dye reacts with amine groups to form a stable amide bond. AATOM™ 565 Acid, 5-Isomer demonstrates strong absorption, high fluorescence quantum yield, and good thermal and photostability. However, it has low water solubility. For applications requiring high water solubility, iFluor® 568 acid is recommended as an alternative. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 565 Acid, 5-Isomer to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	160.1 µL	800.5 µL	1.601 mL	8.005 mL	16.01 mL
5 mM	32.02 µL	160.1 µL	320.2 µL	1.601 mL	3.202 mL
10 mM	16.01 µL	80.05 µL	160.1 µL	800.5 µL	1.601 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	624.61 g/mol	=	mL	x	mM	=	mmol

References

View all 7 references: Citation Explorer

The new live imagers MitoMM1/2 for mitochondrial visualization.

Authors:

Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi

Journal:

Biochemical and biophysical research communications (2021): 50-54

DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission.

Authors:

Ucar, Hülya and Wagenknecht, Hans-Achim

Journal:

Chemical science (2021): 10048-10053

Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.

Authors:

Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther

Journal:

Scientific reports (2021): 14364

Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.

Authors:

Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin

Journal:

Bioanalysis (2019): 1917-1925

The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling.

Authors:

Babcock, Jeremiah J and Brancaleon, Lorenzo

Journal:

Biopolymers (2015): 285-95

Physical properties

Molecular weight	624.61
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.27
Correction factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	120000
Excitation (nm)	562
Emission (nm)	589
Quantum yield	0.90

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 5, 2026