AATOM™ 590 maleimide

AATOM™ 590 maleimide is a thiol‑reactive, orange‑red fluorescent dye with an excitation/emission of 592/621 nm that is used for FACS and FISH applications.

Thiol‑Selective Conjugation: Enables robust labeling of thiol‑modified oligonucleotides, peptides, and small ligands
High Quantum Yield & Photostability: Produces bright fluorescence with high thermal resilience
FRET Compatible: Fluorescence quenched effectively by BHQ®‑2 for FRET and molecular beacon assays

The chemical structure of AATOM™ 590 maleimide.

Protocol

SDS

Catalog	Size	Price	Quantity
70242	1 mg	Price

Overview

AATOM™ 590 is a rhodamine-based fluorescent dye characterized by its strong absorption, high fluorescence quantum yield, and excellent photostability and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 575-610 nm wavelength range. AATOM™ 590 emits in the orange-red region of the visible spectrum, with fluorescence effectively quenched by BHQ®-2 dye. This dye is particularly suited for advanced applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED microscopy. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays. AATOM™ 590 is a suitable alternative to Alexa Fluor® 594 for these applications.

The maleimide derivative of AATOM™ 590 is widely used for labeling biomolecules with free thiol (SH) groups, including peptides and oligonucleotides. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 590 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	122.956 µL	614.779 µL	1.23 mL	6.148 mL	12.296 mL
5 mM	24.591 µL	122.956 µL	245.912 µL	1.23 mL	2.459 mL
10 mM	12.296 µL	61.478 µL	122.956 µL	614.779 µL	1.23 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	813.3 g/mol	=	mL	x	mM	=	mmol

References

View all 17 references: Citation Explorer

Closing the green gap of photosystem I with synthetic fluorophores for enhanced photocurrent generation in photobiocathodes.

Authors:

Morlock, Sascha and Subramanian, Senthil K and Zouni, Athina and Lisdat, Fred

Journal:

Chemical science (2023): 1696-1708

A Multicolor Large Stokes Shift Fluorogen-Activating RNA Aptamer with Cationic Chromophores.

Authors:

Steinmetzger, Christian and Palanisamy, Navaneethan and Gore, Kiran R and Höbartner, Claudia

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 1931-1935

Diffusional Dynamics of Tetraalkylphosphonium Ionic Liquid Films Measured by Fluorescence Correlation Spectroscopy.

Authors:

Mendivelso-Pérez, Deyny L and Farooq, Muhammad Qamar and Santra, Kalyan and Anderson, Jared L and Petrich, Jacob W and Smith, Emily A

Journal:

The journal of physical chemistry. B (2019): 4943-4949

Site-selective functionalization of plasmonic nanopores for enhanced fluorescence emission rate and Förster resonance energy transfer.

Authors:

Zambrana-Puyalto, Xavier and Maccaferri, Nicolò and Ponzellini, Paolo and Giovannini, Giorgia and De Angelis, Francesco and Garoli, Denis

Journal:

Nanoscale advances (2019): 2454-2461

A CE-FL based method for real-time detection of in-capillary self-assembly of the nanoconjugates of polycysteine ligand and quantum dots.

Authors:

Wang, Jianhao and Zhu, Zhilan and Qiu, Lin and Wang, Jianpeng and Wang, Xiang and Xiao, Qicai and Xia, Jiang and Liu, Li and Liu, Xiaoqian and Feng, Wei and Wang, Jinmei and Miao, Peng and Gao, Liqian

Journal:

Nanotechnology (2018): 274001

Physical properties

Molecular weight	813.30
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.39
Correction factor (280 nm)	0.43
Extinction coefficient (cm ^-1 M ^-1)	120000
Excitation (nm)	592
Emission (nm)	621
Quantum yield	0.80

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on July 15, 2026