AATOM™ 590 TCO

AATOM™ 590 TCO is a tetrazine-reactive, orange-red fluorescent dye with an excitation/emission of 592/621 nm that is used for FACS and FISH applications.

Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules with high yield
High Quantum Yield & Stability: Delivers bright fluorescence with high photostability and thermal resilience
Compatibility with Quenchers: Fluorescence effectively quenched by BHQ®-2 dye, enabling precise FRET applications and molecular beacon assays

The chemical structure of AATOM™ 590 TCO.

Datasheet

SDS

Catalog	Size	Price	Quantity
70246	1 mg	Price

Overview

AATOM™ 590 is a rhodamine-based fluorescent dye characterized by its strong absorption, high fluorescence quantum yield, and excellent photostability and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 575–610 nm wavelength range. AATOM™ 590 emits in the orange-red region of the visible spectrum, with fluorescence effectively quenched by BHQ®-2 dye. This dye is particularly suited for advanced applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED microscopy. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays. AATOM™ 590 is a suitable alternative to Alexa Fluor® 594 for these applications.

AATOM™ 590 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 590 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	111.175 µL	555.877 µL	1.112 mL	5.559 mL	11.118 mL
5 mM	22.235 µL	111.175 µL	222.351 µL	1.112 mL	2.224 mL
10 mM	11.118 µL	55.588 µL	111.175 µL	555.877 µL	1.112 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	899.48 g/mol	=	mL	x	mM	=	mmol

References

View all 17 references: Citation Explorer

Closing the green gap of photosystem I with synthetic fluorophores for enhanced photocurrent generation in photobiocathodes.

Authors:

Morlock, Sascha and Subramanian, Senthil K and Zouni, Athina and Lisdat, Fred

Journal:

Chemical science (2023): 1696-1708

A Multicolor Large Stokes Shift Fluorogen-Activating RNA Aptamer with Cationic Chromophores.

Authors:

Steinmetzger, Christian and Palanisamy, Navaneethan and Gore, Kiran R and Höbartner, Claudia

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 1931-1935

Diffusional Dynamics of Tetraalkylphosphonium Ionic Liquid Films Measured by Fluorescence Correlation Spectroscopy.

Authors:

Mendivelso-Pérez, Deyny L and Farooq, Muhammad Qamar and Santra, Kalyan and Anderson, Jared L and Petrich, Jacob W and Smith, Emily A

Journal:

The journal of physical chemistry. B (2019): 4943-4949

Site-selective functionalization of plasmonic nanopores for enhanced fluorescence emission rate and Förster resonance energy transfer.

Authors:

Zambrana-Puyalto, Xavier and Maccaferri, Nicolò and Ponzellini, Paolo and Giovannini, Giorgia and De Angelis, Francesco and Garoli, Denis

Journal:

Nanoscale advances (2019): 2454-2461

A CE-FL based method for real-time detection of in-capillary self-assembly of the nanoconjugates of polycysteine ligand and quantum dots.

Authors:

Wang, Jianhao and Zhu, Zhilan and Qiu, Lin and Wang, Jianpeng and Wang, Xiang and Xiao, Qicai and Xia, Jiang and Liu, Li and Liu, Xiaoqian and Feng, Wei and Wang, Jinmei and Miao, Peng and Gao, Liqian

Journal:

Nanotechnology (2018): 274001

Physical properties

Molecular weight	899.48
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.39
Correction factor (280 nm)	0.43
Extinction coefficient (cm ^-1 M ^-1)	120000
Excitation (nm)	592
Emission (nm)	621
Quantum yield	0.80

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 3, 2026