AATOM™ 594 acid

AATOM™ 594 acid is a non-reactive, red fluorescent dye with an excitation/emission of 602/621 nm that is used for FACS and FISH applications.

High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
Superior Water Solubility: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

ATTO 594 is a fluorescent rhodamine dye. It has strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. The AATOM™ 594 acid can be conjugated to biomolecules with activation by EDC or other catalysts.

Datasheet

SDS

Catalog	Size	Price	Quantity
2858	10 mg	Price

Overview

AATOM™ 594 is a bright, red fluorescent dye characterized by a strong absorption, high fluorescence quantum yield, and exceptional thermal and photostability. The dye exhibits superior water solubility and hydrophilicity, facilitating its use in various aqueous environments. AATOM™ 594 is optimally excited within the 560–615 nm range, making it compatible with both 561 nm and 594 nm laser lines commonly used in advanced fluorescence imaging systems. Upon conjugation to biomolecules, AATOM™ 594 becomes anionic, carrying a net charge of -1, which may influence its binding characteristics and performance in assays. Its photostability and brightness make it particularly suited for high-resolution techniques like single-molecule detection and super-resolution microscopy, including PALM, dSTORM, and STED. Additionally, AATOM™ 594 is highly compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other fluorescence-based assays, supporting its broad utility in complex biological studies.

AATOM™ 594 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing AATOM™ 594 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 594 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	99.172 µL	495.86 µL	991.719 µL	4.959 mL	9.917 mL
5 mM	19.834 µL	99.172 µL	198.344 µL	991.719 µL	1.983 mL
10 mM	9.917 µL	49.586 µL	99.172 µL	495.86 µL	991.719 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1008.35 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells.

Authors:

Xiao, Wen and Yeom, Kyu-Hyeon and Lin, Chia-Ho and Black, Douglas L

Journal:

RNA (New York, N.Y.) (2023)

Measuring Photophysical Transition Rates with Fluorescence Correlation Spectroscopy and Antibunching.

Authors:

Sakhapov, Damir and Gregor, Ingo and Karedla, Narain and Enderlein, Jörg

Journal:

The journal of physical chemistry letters (2022): 4823-4830

Combining Fluorescence Fluctuations and Photobleaching to Quantify Surface Density.

Authors:

Sefkow-Werner, Julius and Migliorini, Elisa and Picart, Catherine and Wahyuni, Dwiria and Wang, Irène and Delon, Antoine

Journal:

Analytical chemistry (2022): 6521-6528

A Model of F-actin Organization in Granuloreticulopodia in Foraminifera: Morphogenetic and Evolutionary Implications from Novel Fluorescent and Polarised Light Observations.

Authors:

Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max

Journal:

Protist (2022): 125886

Fractional CO2 laser ablation leads to enhanced permeation of a fluorescent dye in healthy and mycotic nails-An imaging investigation of laser-tissue effects and their impact on ungual drug delivery.

Authors:

Ortner, Vinzent Kevin and Nguyen, Nhi and Brewer, Jonathan R and Solovyeva, Vita and Haedersdal, Merete and Philipsen, Peter Alshede

Journal:

Lasers in surgery and medicine (2022): 861-874

Physical properties

Molecular weight	1008.35
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.26
Correction factor (280 nm)	0.51
Extinction coefficient (cm ^-1 M ^-1)	120000
Excitation (nm)	602
Emission (nm)	621
Quantum yield	0.85

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 28, 2026