AATOM™ 594 is a bright, red fluorescent dye characterized by a strong absorption, high fluorescence quantum yield, and exceptional thermal and photostability. The dye exhibits superior water solubility and hydrophilicity, facilitating its use in various aqueous environments. AATOM™ 594 is optimally excited within the 560-615 nm range, making it compatible with both 561 nm and 594 nm laser lines commonly used in advanced fluorescence imaging systems. Upon conjugation to biomolecules, AATOM™ 594 becomes anionic, carrying a net charge of -1, which may influence its binding characteristics and performance in assays. Its photostability and brightness make it particularly suited for high-resolution techniques like single-molecule detection and super-resolution microscopy, including PALM, dSTORM, and STED. Additionally, AATOM™ 594 is highly compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other fluorescence-based assays, supporting its broad utility in complex biological studies.

The maleimide derivative of AATOM™ 594 is widely used for labeling biomolecules with free thiol (SH) groups, including peptides and oligonucleotides. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.