AATOM™ 610 azide

AATOM™ 610 azide is an alkyl-reactive, red fluorescent dye with an excitation/emission of 615/632 nm that is used for FACS and FISH applications.

Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
High Quantum Yield & Stability: Offers bright, stable fluorescence with excellent photostability and thermal stability for sensitive imaging applications
Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

The chemical structure of AATOM™ 610 azide.

Datasheet

SDS

Catalog	Size	Price	Quantity
70253	1 mg	Price

Overview

AATOM™ 610 is a carbopyronin-based fluorescent dye known for its strong absorption, high fluorescence quantum yield, and exceptional photostability and thermal stability. It is moderately hydrophilic and optimally excited at wavelengths between 595 and 625 nm. Upon coupling to a substrate, AATOM™ 610 becomes cationic, carrying a net electrical charge of +1. The dye remains stable under physiological pH conditions and in buffers with a pH of up to 8, though it gradually degrades at higher pH levels. AATOM™ 610 is ideal for advanced applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays.

The azide derivative of AATOM™ 610 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 610 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	141.886 µL	709.431 µL	1.419 mL	7.094 mL	14.189 mL
5 mM	28.377 µL	141.886 µL	283.772 µL	1.419 mL	2.838 mL
10 mM	14.189 µL	70.943 µL	141.886 µL	709.431 µL	1.419 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	704.79 g/mol	=	mL	x	mM	=	mmol

References

View all 4 references: Citation Explorer

Two-Photon-Excited Single-Molecule Fluorescence Enhanced by Gold Nanorod Dimers.

Authors:

Lu, Xuxing and Punj, Deep and Orrit, Michel

Journal:

Nano letters (2022): 4215-4222

Ultrabright Terbium Nanoparticles for FRET Biosensing and in Situ Imaging of Epidermal Growth Factor Receptors*.

Authors:

Charpentier, Cyrille and Cifliku, Vjona and Goetz, Joan and Nonat, Aline and Cheignon, Clémence and Cardoso Dos Santos, Marcelina and Francés-Soriano, Laura and Wong, Ka-Leung and Charbonnière, Loïc J and Hildebrandt, Niko

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 14602-14611

Oxazine dye-conjugated dna oligonucleotides: Förster resonance energy transfer in view of molecular dye-DNA interactions.

Authors:

Kupstat, Annette and Ritschel, Thomas and Kumke, Michael U

Journal:

Bioconjugate chemistry (2011): 2546-57

Novel wavelength-resolved fluorescence detection for a high-throughput capillary electrophoresis system under a diascopic configuration.

Authors:

Lin, Shi-Wei and Chang, Guan-Liang and Lin, Che-Hsin

Journal:

Journal of chromatography. A (2008): 198-201

Physical properties

Molecular weight	704.79
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.06
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	615
Emission (nm)	632
Quantum yield	0.70

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on May 3, 2026