AATOM™ 610 Tetrazine

AATOM™ 610 tetrazine is a TCO-reactive, red fluorescent dye with an excitation/emission of 615/632 nm that is used for FACS and FISH applications.

Rapid Conjugation: Ideal for labeling TCO-modified biomolecules under copper-free conditions, providing fast reaction kinetics
High Quantum Yield & Stability: Offers bright, stable fluorescence with excellent photostability and thermal stability for sensitive imaging applications
Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

The chemical structure of ATTO 610 tetrazine

Datasheet

SDS

Catalog	Size	Price	Quantity
70257	1 mg	Price

Overview

AATOM™ 610 is a carbopyronin-based fluorescent dye known for its strong absorption, high fluorescence quantum yield, and exceptional photostability and thermal stability. It is moderately hydrophilic and optimally excited at wavelengths between 595 and 625 nm. Upon coupling to a substrate, AATOM™ 610 becomes cationic, carrying a net electrical charge of +1. The dye remains stable under physiological pH conditions and in buffers with a pH of up to 8, though it gradually degrades at higher pH levels. AATOM™ 610 is ideal for advanced applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays.

AATOM™ 610 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 610 Tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	145.397 µL	726.987 µL	1.454 mL	7.27 mL	14.54 mL
5 mM	29.079 µL	145.397 µL	290.795 µL	1.454 mL	2.908 mL
10 mM	14.54 µL	72.699 µL	145.397 µL	726.987 µL	1.454 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	687.77 g/mol	=	mL	x	mM	=	mmol

References

View all 4 references: Citation Explorer

Two-Photon-Excited Single-Molecule Fluorescence Enhanced by Gold Nanorod Dimers.

Authors:

Lu, Xuxing and Punj, Deep and Orrit, Michel

Journal:

Nano letters (2022): 4215-4222

Ultrabright Terbium Nanoparticles for FRET Biosensing and in Situ Imaging of Epidermal Growth Factor Receptors*.

Authors:

Charpentier, Cyrille and Cifliku, Vjona and Goetz, Joan and Nonat, Aline and Cheignon, Clémence and Cardoso Dos Santos, Marcelina and Francés-Soriano, Laura and Wong, Ka-Leung and Charbonnière, Loïc J and Hildebrandt, Niko

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 14602-14611

Oxazine dye-conjugated dna oligonucleotides: Förster resonance energy transfer in view of molecular dye-DNA interactions.

Authors:

Kupstat, Annette and Ritschel, Thomas and Kumke, Michael U

Journal:

Bioconjugate chemistry (2011): 2546-57

Novel wavelength-resolved fluorescence detection for a high-throughput capillary electrophoresis system under a diascopic configuration.

Authors:

Lin, Shi-Wei and Chang, Guan-Liang and Lin, Che-Hsin

Journal:

Journal of chromatography. A (2008): 198-201

Physical properties

Molecular weight	687.77
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.06
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	615
Emission (nm)	632
Quantum yield	0.70

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on May 1, 2026