AATOM™ 620 is a bright-red fluorescent dye, characterized by its strong absorption, exceptional photo and thermal stability, and temperature-dependent fluorescence. It demonstrates moderate hydrophilicity and optimal excitation within the 600–630 nm wavelength range. The dye maintains stable fluorescence over a wide pH range (2–11), allowing for its use in diverse experimental conditions. Upon conjugation to a substrate, AATOM™ 620 becomes cationic, carrying a net positive charge of +1. These properties make AATOM™ 620 particularly suitable for high-precision applications, including single-molecule detection and super-resolution microscopy techniques such as PALM, dSTORM, and STED. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

AATOM™ 620 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.