AATOM™ 655 is a far-red fluorescent dye characterized by its strong absorption, high photo and thermal stability, excellent ozone resistance and excellent water solubility. The dye exhibits moderate hydrophilicity and is optimally excited within the 640–660 nm wavelength range, which aligns with the 647 nm line of Krypton-Ion lasers and the 650 nm line of diode lasers. As a zwitterionic compound, AATOM™ 655 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties render AATOM™ 655 highly suitable for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, AATOM™ 655 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.

AATOM™ 655 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing AATOM™ 655 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.