AATOM™ 655 is a far-red fluorescent dye characterized by its strong absorption, high photo and thermal stability, excellent ozone resistance, and excellent water solubility. The dye exhibits moderate hydrophilicity and is optimally excited within the 640-660 nm wavelength range, which aligns with the 647 nm line of Krypton-Ion lasers and the 650 nm line of diode lasers. As a zwitterionic compound, AATOM™ 655 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties render AATOM™ 655 highly suitable for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, AATOM™ 655 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.

The maleimide derivative of AATOM™ 655 is widely used for labeling biomolecules with free thiol (SH) groups, including peptides and oligonucleotides. Maleimides react readily with sulfhydryl groups, forming stable thio-ether bonds between the dye and the biomolecule, facilitating robust and reliable labeling for diverse experimental applications. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.