AATOM™ 680 is a far-red fluorescent dye characterized by its strong absorption, high photo and thermal stability, and good aqueous solubility. It is optimally excited within the 645-695 nm wavelength range, which aligns with the 670 nm line of diode laser and the 676 nm line of Krypton-Ion laser. As a zwitterionic compound, AATOM™ 680 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties make AATOM™ 680 ideal for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, AATOM™ 680 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.

NHS esters react selectively and efficiently with primary amines (such as the side chains of lysine residues or aminosilane-coated surfaces) at pH 7-9, forming stable covalent amide bonds. This property makes AATOM™ 680 NHS ester sutiable for labeling peptides and amine-modified oligonucleotides. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.